ENSG00000162104

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000294016 ENSG00000162104 HEK293_OSMI2_2hA HEK293_TMG_2hB ADCY9 protein_coding protein_coding 6.847691 6.399035 6.52302 0.7338437 0.1551238 0.02764308 5.7548784 5.77980146 5.1970291 0.62092740 0.05095246 -0.1530534 0.83783750 0.905033333 0.7972667 -0.10776667 0.002551983 0.00101619 FALSE TRUE
ENST00000571467 ENSG00000162104 HEK293_OSMI2_2hA HEK293_TMG_2hB ADCY9 protein_coding nonsense_mediated_decay 6.847691 6.399035 6.52302 0.7338437 0.1551238 0.02764308 0.2092926 0.00000000 0.3340540 0.00000000 0.12193127 5.1045633 0.03132083 0.000000000 0.0504000 0.05040000 0.001016190 0.00101619 TRUE TRUE
ENST00000572288 ENSG00000162104 HEK293_OSMI2_2hA HEK293_TMG_2hB ADCY9 protein_coding protein_coding 6.847691 6.399035 6.52302 0.7338437 0.1551238 0.02764308 0.1321760 0.06234707 0.4479779 0.06234707 0.24231832 2.6622716 0.02029167 0.008733333 0.0702000 0.06146667 0.506851574 0.00101619 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000162104 E001 1.5489877 0.0120991133 5.786465e-01 7.036960e-01 16 3953387 3953516 130 - 0.428 0.355 -0.408
ENSG00000162104 E002 754.3453258 0.0046660367 6.278383e-07 5.445817e-06 16 3962653 3966526 3874 - 2.781 2.934 0.512
ENSG00000162104 E003 55.0835676 0.0004725583 1.945513e-01 3.194416e-01 16 3966527 3966631 105 - 1.748 1.728 -0.070
ENSG00000162104 E004 91.6857442 0.0003588266 8.744224e-02 1.712621e-01 16 3966632 3966966 335 - 1.970 1.948 -0.072
ENSG00000162104 E005 26.1029699 0.0007495099 7.973273e-01 8.706688e-01 16 3974669 3974710 42 - 1.412 1.435 0.078
ENSG00000162104 E006 40.5238118 0.0005185439 9.023684e-01 9.418164e-01 16 3977482 3977630 149 - 1.590 1.624 0.114
ENSG00000162104 E007 39.9767357 0.0005354637 7.175554e-01 8.129069e-01 16 3979116 3979275 160 - 1.590 1.611 0.070
ENSG00000162104 E008 6.0625556 0.0430178981 1.009896e-01 1.919756e-01 16 3981773 3983231 1459 - 0.671 0.960 1.137
ENSG00000162104 E009 45.2834373 0.0004909917 3.292323e-01 4.727413e-01 16 3983232 3983391 160 - 1.601 1.693 0.312
ENSG00000162104 E010 32.2489058 0.0006649958 2.459949e-01 3.812586e-01 16 3983392 3983440 49 - 1.445 1.557 0.385
ENSG00000162104 E011 0.1482932 0.0428664526 5.606531e-01   16 3983441 3984150 710 - 0.000 0.119 9.380
ENSG00000162104 E012 40.1403794 0.0005084608 7.331937e-01 8.244528e-01 16 3988994 3989083 90 - 1.571 1.630 0.202
ENSG00000162104 E013 20.0367600 0.0009182060 9.032507e-01 9.424310e-01 16 3989084 3989088 5 - 1.296 1.327 0.105
ENSG00000162104 E014 21.0645334 0.0008667750 7.991422e-01 8.718661e-01 16 3989089 3989096 8 - 1.325 1.345 0.071
ENSG00000162104 E015 38.5119499 0.0065937968 7.258076e-01 8.190009e-01 16 3992146 3992221 76 - 1.575 1.597 0.074
ENSG00000162104 E016 29.9724743 0.0163028694 8.520664e-01 9.083009e-01 16 3992222 3992270 49 - 1.460 1.498 0.129
ENSG00000162104 E017 40.6899576 0.0061977668 7.162607e-02 1.460451e-01 16 3992271 3992363 93 - 1.649 1.575 -0.252
ENSG00000162104 E018 0.9879930 0.0137935971 3.175754e-01 4.604742e-01 16 3992364 3992367 4 - 0.370 0.213 -1.087
ENSG00000162104 E019 6.0580297 0.0027955573 7.590952e-01 8.435350e-01 16 3993349 3993405 57 - 0.848 0.845 -0.011
ENSG00000162104 E020 44.1607879 0.0031200817 1.557254e-03 5.820486e-03 16 3993406 3993510 105 - 1.719 1.577 -0.481
ENSG00000162104 E021 53.0450985 0.0069679141 1.987815e-02 5.123126e-02 16 4007368 4007558 191 - 1.783 1.680 -0.348
ENSG00000162104 E022 0.0000000       16 4015562 4015682 121 -      
ENSG00000162104 E023 0.0000000       16 4054035 4054190 156 -      
ENSG00000162104 E024 249.8128891 0.0020341135 1.454580e-09 2.090953e-08 16 4113750 4115485 1736 - 2.459 2.335 -0.412
ENSG00000162104 E025 21.1549423 0.0009186179 1.378685e-05 8.867050e-05 16 4115690 4116442 753 - 1.475 1.191 -0.991