ENSG00000162086

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000498240 ENSG00000162086 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF75A protein_coding nonsense_mediated_decay 8.07752 4.055262 9.088643 0.2698364 0.2648215 1.162303 0.6965891 0.1932201 0.5506250 0.10585365 0.2869869 1.4639933 0.09513333 0.04973333 0.05943333 0.00970000 1.00000000 0.02221532 TRUE TRUE
ENST00000571101 ENSG00000162086 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF75A protein_coding processed_transcript 8.07752 4.055262 9.088643 0.2698364 0.2648215 1.162303 0.2500161 0.6401373 0.2919190 0.32677431 0.2919190 -1.1065832 0.03687917 0.15876667 0.03306667 -0.12570000 0.44092655 0.02221532 FALSE TRUE
ENST00000572999 ENSG00000162086 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF75A protein_coding retained_intron 8.07752 4.055262 9.088643 0.2698364 0.2648215 1.162303 1.0642600 0.4504195 1.0174539 0.12616797 0.1866033 1.1580529 0.13261250 0.11130000 0.11330000 0.00200000 1.00000000 0.02221532   FALSE
ENST00000573828 ENSG00000162086 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF75A protein_coding processed_transcript 8.07752 4.055262 9.088643 0.2698364 0.2648215 1.162303 0.7805989 0.0000000 1.1781751 0.00000000 0.6530603 6.8926036 0.07823333 0.00000000 0.12653333 0.12653333 0.28021495 0.02221532   FALSE
ENST00000575253 ENSG00000162086 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF75A protein_coding retained_intron 8.07752 4.055262 9.088643 0.2698364 0.2648215 1.162303 0.6572325 0.3087671 0.4760901 0.07958140 0.1076716 0.6087210 0.08233333 0.07493333 0.05190000 -0.02303333 0.65664998 0.02221532 FALSE TRUE
ENST00000575812 ENSG00000162086 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF75A protein_coding processed_transcript 8.07752 4.055262 9.088643 0.2698364 0.2648215 1.162303 0.6327864 0.3681716 0.4802581 0.07838801 0.1562029 0.3745005 0.08430417 0.09166667 0.05390000 -0.03776667 0.55430029 0.02221532 FALSE TRUE
ENST00000612186 ENSG00000162086 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF75A protein_coding protein_coding 8.07752 4.055262 9.088643 0.2698364 0.2648215 1.162303 1.6944458 0.7775142 1.9430182 0.10053905 0.2214226 1.3103275 0.20068750 0.19163333 0.21330000 0.02166667 0.85444684 0.02221532 FALSE TRUE
ENST00000669516 ENSG00000162086 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF75A protein_coding protein_coding 8.07752 4.055262 9.088643 0.2698364 0.2648215 1.162303 0.8609578 0.1060863 1.9727562 0.07919458 0.3711004 4.0942381 0.09038333 0.02863333 0.21963333 0.19100000 0.02221532 0.02221532 FALSE TRUE
MSTRG.11931.8 ENSG00000162086 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF75A protein_coding   8.07752 4.055262 9.088643 0.2698364 0.2648215 1.162303 1.1367751 1.1331677 0.5697596 0.24394195 0.3218183 -0.9795104 0.16429167 0.27406667 0.06453333 -0.20953333 0.24743935 0.02221532 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000162086 E001 0.2966881 0.029553430 4.722809e-01   16 3305406 3305471 66 + 0.162 0.000 -9.710
ENSG00000162086 E002 0.2966881 0.029553430 4.722809e-01   16 3305472 3305483 12 + 0.162 0.000 -11.795
ENSG00000162086 E003 0.5858876 0.020899502 7.389028e-01 8.286691e-01 16 3305484 3305496 13 + 0.225 0.166 -0.540
ENSG00000162086 E004 1.4758382 0.009276955 9.155386e-02 1.775601e-01 16 3305497 3305507 11 + 0.483 0.167 -2.124
ENSG00000162086 E005 12.7827423 0.001347776 7.618000e-04 3.121190e-03 16 3305508 3305643 136 + 1.227 0.878 -1.276
ENSG00000162086 E006 8.0497992 0.002170394 4.507060e-01 5.917712e-01 16 3305644 3308312 2669 + 0.904 0.995 0.341
ENSG00000162086 E007 6.2826813 0.002623168 1.806519e-02 4.727067e-02 16 3308313 3308331 19 + 0.951 0.631 -1.276
ENSG00000162086 E008 29.5531362 0.007374373 4.253138e-05 2.436628e-04 16 3308332 3308737 406 + 1.566 1.239 -1.129
ENSG00000162086 E009 16.3399072 0.001191802 1.964222e-02 5.072120e-02 16 3308738 3308836 99 + 1.291 1.087 -0.726
ENSG00000162086 E010 28.7742217 0.002304927 9.446410e-01 9.692500e-01 16 3308837 3309472 636 + 1.453 1.464 0.036
ENSG00000162086 E011 17.0380242 0.032488453 4.833353e-01 6.213456e-01 16 3310611 3310946 336 + 1.203 1.289 0.306
ENSG00000162086 E012 6.7774128 0.008858464 7.012435e-01 8.004897e-01 16 3311620 3311752 133 + 0.852 0.905 0.201
ENSG00000162086 E013 13.1679143 0.001363946 4.340646e-01 5.764501e-01 16 3311753 3311789 37 + 1.157 1.087 -0.250
ENSG00000162086 E014 24.4246887 0.000925013 9.958868e-01 1.000000e+00 16 3311790 3311948 159 + 1.390 1.395 0.018
ENSG00000162086 E015 6.2115964 0.002783731 1.330011e-01 2.382889e-01 16 3311949 3312365 417 + 0.760 0.952 0.742
ENSG00000162086 E016 21.4177982 0.001234507 3.457929e-01 4.899257e-01 16 3312677 3312768 92 + 1.361 1.295 -0.232
ENSG00000162086 E017 15.5304113 0.010907390 3.082108e-02 7.356692e-02 16 3313049 3313175 127 + 1.271 1.053 -0.781
ENSG00000162086 E018 9.0030849 0.019407569 6.762829e-01 7.813021e-01 16 3315991 3316911 921 + 0.961 1.017 0.206
ENSG00000162086 E019 26.5359315 0.016921063 7.374008e-01 8.275921e-01 16 3316912 3317022 111 + 1.432 1.411 -0.074
ENSG00000162086 E020 158.1258155 0.001703013 8.389831e-08 8.646186e-07 16 3317190 3318852 1663 + 2.125 2.267 0.477