ENSG00000162073

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000293978 ENSG00000162073 HEK293_OSMI2_2hA HEK293_TMG_2hB PAQR4 protein_coding protein_coding 39.45702 61.11702 27.06983 2.719177 1.020543 -1.174592 2.100008 2.430895 2.594934 0.3744463 0.9056578 0.09383669 0.0543375 0.03936667 0.0945 0.05513333 3.402303e-01 4.896769e-17 FALSE TRUE
ENST00000318782 ENSG00000162073 HEK293_OSMI2_2hA HEK293_TMG_2hB PAQR4 protein_coding protein_coding 39.45702 61.11702 27.06983 2.719177 1.020543 -1.174592 15.025745 20.384377 13.293955 3.3421939 0.2046308 -0.61631626 0.3887417 0.33250000 0.4921 0.15960000 8.587383e-02 4.896769e-17 FALSE TRUE
ENST00000574988 ENSG00000162073 HEK293_OSMI2_2hA HEK293_TMG_2hB PAQR4 protein_coding protein_coding 39.45702 61.11702 27.06983 2.719177 1.020543 -1.174592 15.645408 16.602617 10.404074 3.8517801 0.6524675 -0.67374471 0.4200833 0.26716667 0.3837 0.11653333 2.789781e-01 4.896769e-17 FALSE TRUE
ENST00000576565 ENSG00000162073 HEK293_OSMI2_2hA HEK293_TMG_2hB PAQR4 protein_coding protein_coding 39.45702 61.11702 27.06983 2.719177 1.020543 -1.174592 5.183534 17.975694 0.000000 3.9901468 0.0000000 -10.81263410 0.1016625 0.29893333 0.0000 -0.29893333 4.896769e-17 4.896769e-17 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000162073 E001 4.848252 0.1184279149 1.189060e-03 4.604457e-03 16 2969270 2969340 71 + 1.058 0.453 -2.506
ENSG00000162073 E002 4.666548 0.1538821971 1.498255e-03 5.630339e-03 16 2969341 2969347 7 + 1.058 0.418 -2.689
ENSG00000162073 E003 24.840275 0.0321585690 9.483395e-15 3.127509e-13 16 2969348 2969443 96 + 1.771 0.953 -2.860
ENSG00000162073 E004 45.046698 0.0139007728 3.124193e-15 1.108369e-13 16 2969444 2969557 114 + 1.928 1.378 -1.872
ENSG00000162073 E005 215.275307 0.0080857317 1.721834e-03 6.351172e-03 16 2969558 2969840 283 + 2.354 2.258 -0.322
ENSG00000162073 E006 42.274837 0.0005382628 1.010876e-02 2.902948e-02 16 2970978 2971156 179 + 1.651 1.553 -0.333
ENSG00000162073 E007 214.172285 0.0013189745 8.971115e-01 9.382941e-01 16 2971157 2971273 117 + 2.248 2.296 0.159
ENSG00000162073 E008 260.893645 0.0002520490 4.860831e-01 6.239040e-01 16 2971274 2971378 105 + 2.317 2.385 0.228
ENSG00000162073 E009 2675.616584 0.0043612521 5.350372e-06 3.782416e-05 16 2971515 2973484 1970 + 3.309 3.405 0.321