ENSG00000161547

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000359995 ENSG00000161547 HEK293_OSMI2_2hA HEK293_TMG_2hB SRSF2 protein_coding protein_coding 416.6683 533.4837 494.2854 34.46542 8.162519 -0.1100978 297.91869 465.059366 377.33321 31.722643 10.505763 -0.3015686 0.68687083 0.871433333 0.76313333 -0.10830000 3.466453e-08 6.024134e-35 FALSE TRUE
ENST00000392485 ENSG00000161547 HEK293_OSMI2_2hA HEK293_TMG_2hB SRSF2 protein_coding protein_coding 416.6683 533.4837 494.2854 34.46542 8.162519 -0.1100978 28.97752 14.297451 42.80180 1.614880 1.878233 1.5812419 0.07253750 0.026733333 0.08673333 0.06000000 6.466391e-16 6.024134e-35 FALSE TRUE
ENST00000585202 ENSG00000161547 HEK293_OSMI2_2hA HEK293_TMG_2hB SRSF2 protein_coding nonsense_mediated_decay 416.6683 533.4837 494.2854 34.46542 8.162519 -0.1100978 31.33446 24.236719 29.68348 3.152982 2.223303 0.2923568 0.08153750 0.046300000 0.06016667 0.01386667 5.294461e-01 6.024134e-35 TRUE TRUE
ENST00000586778 ENSG00000161547 HEK293_OSMI2_2hA HEK293_TMG_2hB SRSF2 protein_coding retained_intron 416.6683 533.4837 494.2854 34.46542 8.162519 -0.1100978 20.50112 14.172364 12.74611 3.753743 2.129185 -0.1529095 0.05615833 0.025933333 0.02573333 -0.00020000 1.000000e+00 6.024134e-35   FALSE
ENST00000592676 ENSG00000161547 HEK293_OSMI2_2hA HEK293_TMG_2hB SRSF2 protein_coding retained_intron 416.6683 533.4837 494.2854 34.46542 8.162519 -0.1100978 19.88814 1.660331 17.10642 1.660331 1.155865 3.3571758 0.05518333 0.003333333 0.03456667 0.03123333 4.502760e-02 6.024134e-35   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000161547 E001 32.23053 0.0382766742 4.305405e-06 3.108526e-05 17 76734115 76734122 8 - 1.070 1.692 2.164
ENSG00000161547 E002 105.81478 0.0484265573 8.443926e-05 4.490320e-04 17 76734123 76734146 24 - 1.650 2.179 1.780
ENSG00000161547 E003 158.59865 0.0324146715 1.905677e-06 1.489438e-05 17 76734147 76734162 16 - 1.834 2.351 1.731
ENSG00000161547 E004 1323.53409 0.0076375373 4.291950e-04 1.886886e-03 17 76734163 76734251 89 - 2.991 3.191 0.663
ENSG00000161547 E005 2092.43041 0.0083631645 2.002324e-02 5.153788e-02 17 76734252 76734302 51 - 3.223 3.373 0.496
ENSG00000161547 E006 5920.08033 0.0076597137 1.866443e-02 4.859437e-02 17 76734303 76734562 260 - 3.683 3.819 0.452
ENSG00000161547 E007 2046.61037 0.0032194536 8.077009e-03 2.397598e-02 17 76734563 76734576 14 - 3.236 3.351 0.384
ENSG00000161547 E008 7869.04776 0.0013493101 1.395528e-06 1.122674e-05 17 76734577 76735060 484 - 3.822 3.935 0.375
ENSG00000161547 E009 4774.66054 0.0001490996 6.831387e-08 7.165637e-07 17 76735061 76735136 76 - 3.630 3.706 0.255
ENSG00000161547 E010 3862.45029 0.0003970101 9.372059e-01 9.645256e-01 17 76735137 76735158 22 - 3.561 3.597 0.120
ENSG00000161547 E011 758.33457 0.0066717761 7.581945e-18 3.777950e-16 17 76735159 76735617 459 - 3.033 2.710 -1.076
ENSG00000161547 E012 321.05517 0.0028276765 1.797059e-47 9.617158e-45 17 76735618 76735771 154 - 2.706 2.260 -1.489
ENSG00000161547 E013 374.47371 0.0003374226 9.092720e-86 1.913987e-82 17 76735772 76735875 104 - 2.744 2.383 -1.203
ENSG00000161547 E014 519.84087 0.0003980238 9.753590e-158 1.154868e-153 17 76735876 76736153 278 - 2.921 2.460 -1.533
ENSG00000161547 E015 6461.82242 0.0009910839 2.171697e-02 5.508810e-02 17 76736154 76736379 226 - 3.805 3.805 -0.002
ENSG00000161547 E016 1898.79506 0.0048648524 3.093003e-02 7.377899e-02 17 76736380 76736404 25 - 3.298 3.254 -0.145
ENSG00000161547 E017 1790.97174 0.0090056210 1.314659e-02 3.624720e-02 17 76736405 76736443 39 - 3.295 3.210 -0.283
ENSG00000161547 E018 1124.81154 0.0130800253 5.020518e-02 1.095195e-01 17 76736444 76736449 6 - 3.091 3.010 -0.269
ENSG00000161547 E019 1201.11222 0.0137370412 4.437379e-02 9.906061e-02 17 76736450 76736464 15 - 3.122 3.036 -0.287
ENSG00000161547 E020 13.76933 0.0070296753 1.396339e-05 8.971146e-05 17 76736465 76736555 91 - 1.346 0.952 -1.413
ENSG00000161547 E021 1387.09648 0.0147187411 7.335199e-02 1.488864e-01 17 76736799 76736834 36 - 3.179 3.103 -0.254
ENSG00000161547 E022 5686.08272 0.0083141908 3.524697e-01 4.967256e-01 17 76736835 76737333 499 - 3.754 3.746 -0.025