ENSG00000161011

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000389805 ENSG00000161011 HEK293_OSMI2_2hA HEK293_TMG_2hB SQSTM1 protein_coding protein_coding 123.1771 212.078 80.00153 13.92654 1.438573 -1.406383 47.246443 86.00908 27.581519 4.668203 0.6116898 -1.6404318 0.37113750 0.4061667 0.34476667 -0.061400000 3.386984e-04 4.893728e-05 FALSE  
ENST00000466342 ENSG00000161011 HEK293_OSMI2_2hA HEK293_TMG_2hB SQSTM1 protein_coding retained_intron 123.1771 212.078 80.00153 13.92654 1.438573 -1.406383 47.492459 84.10990 28.749889 6.546065 1.1426807 -1.5483889 0.38477917 0.3959000 0.35906667 -0.036833333 1.658445e-01 4.893728e-05 FALSE  
ENST00000514093 ENSG00000161011 HEK293_OSMI2_2hA HEK293_TMG_2hB SQSTM1 protein_coding protein_coding 123.1771 212.078 80.00153 13.92654 1.438573 -1.406383 8.880094 10.25602 8.581742 1.377594 0.7344229 -0.2568542 0.08025833 0.0479000 0.10766667 0.059766667 4.893728e-05 4.893728e-05 FALSE  
MSTRG.27457.3 ENSG00000161011 HEK293_OSMI2_2hA HEK293_TMG_2hB SQSTM1 protein_coding   123.1771 212.078 80.00153 13.92654 1.438573 -1.406383 6.693488 12.71561 5.276006 0.867289 0.4115394 -1.2674848 0.05444167 0.0611000 0.06586667 0.004766667 8.680771e-01 4.893728e-05 TRUE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000161011 E001 4.0839302 1.409429e-02 1.014292e-02 2.911564e-02 5 179806398 179806407 10 + 0.894 0.511 -1.609
ENSG00000161011 E002 46.7691182 4.759862e-04 1.192342e-05 7.784060e-05 5 179806408 179806591 184 + 1.767 1.530 -0.806
ENSG00000161011 E003 70.8113268 3.420803e-04 1.349131e-04 6.808050e-04 5 179806962 179807123 162 + 1.898 1.726 -0.579
ENSG00000161011 E004 5.3509149 3.740366e-03 1.733064e-01 2.924972e-01 5 179807324 179808427 1104 + 0.861 0.674 -0.752
ENSG00000161011 E005 142.5665383 3.405907e-04 1.644729e-06 1.302956e-05 5 179811574 179811614 41 + 2.187 2.031 -0.521
ENSG00000161011 E006 178.9585377 2.315878e-04 5.231846e-09 6.802728e-08 5 179811615 179811682 68 + 2.294 2.126 -0.562
ENSG00000161011 E007 8.8033842 2.001828e-03 6.433264e-01 7.559156e-01 5 179811988 179812406 419 + 0.861 0.926 0.247
ENSG00000161011 E008 133.8190099 1.341519e-03 1.111470e-04 5.730431e-04 5 179812407 179813641 1235 + 2.151 2.011 -0.470
ENSG00000161011 E009 0.1515154 4.330409e-02 1.486425e-01   5 179818878 179818928 51 + 0.196 0.000 -10.675
ENSG00000161011 E010 0.9995446 1.276245e-02 6.565211e-01 7.660552e-01 5 179818929 179818966 38 + 0.196 0.285 0.705
ENSG00000161011 E011 2.4711243 6.330874e-03 3.571881e-01 5.015842e-01 5 179818967 179819037 71 + 0.330 0.509 0.970
ENSG00000161011 E012 3.1519495 1.027800e-02 9.915322e-02 1.891879e-01 5 179820104 179820387 284 + 0.745 0.484 -1.155
ENSG00000161011 E013 1.3609511 1.069283e-01 5.443999e-01 6.748774e-01 5 179820607 179820706 100 + 0.196 0.330 1.002
ENSG00000161011 E014 1.5468096 5.247181e-02 5.680099e-01 6.947278e-01 5 179820878 179820884 7 + 0.433 0.326 -0.612
ENSG00000161011 E015 3.1217580 4.915757e-03 7.427182e-01 8.315499e-01 5 179820885 179820888 4 + 0.585 0.534 -0.236
ENSG00000161011 E016 12.1338491 1.103762e-01 6.535171e-01 7.639569e-01 5 179820889 179820904 16 + 0.952 1.052 0.371
ENSG00000161011 E017 162.3294730 1.119275e-02 1.063039e-01 2.000889e-01 5 179820905 179820920 16 + 2.188 2.104 -0.281
ENSG00000161011 E018 613.4126339 1.136844e-02 4.180615e-01 5.613711e-01 5 179820921 179821141 221 + 2.722 2.695 -0.093
ENSG00000161011 E019 11.7073365 6.775110e-03 3.766079e-08 4.159946e-07 5 179821551 179821707 157 + 1.387 0.831 -2.016
ENSG00000161011 E020 40.6353062 3.244179e-02 3.955805e-05 2.282645e-04 5 179822485 179822888 404 + 1.827 1.411 -1.420
ENSG00000161011 E021 15.6563580 1.254728e-02 1.122136e-03 4.377515e-03 5 179822889 179822957 69 + 1.377 1.055 -1.139
ENSG00000161011 E022 612.4788956 3.882572e-03 6.919826e-03 2.101674e-02 5 179822958 179823025 68 + 2.763 2.681 -0.272
ENSG00000161011 E023 450.8466430 1.167664e-03 8.765738e-07 7.367536e-06 5 179823026 179823053 28 + 2.652 2.538 -0.381
ENSG00000161011 E024 15.2774005 8.735810e-03 4.492862e-03 1.452242e-02 5 179823565 179823857 293 + 0.826 1.189 1.345
ENSG00000161011 E025 1413.9907505 1.670625e-03 8.868077e-03 2.595163e-02 5 179823858 179824087 230 + 3.099 3.052 -0.155
ENSG00000161011 E026 1612.2759897 6.420789e-05 5.691307e-01 6.957528e-01 5 179824182 179824323 142 + 3.108 3.121 0.041
ENSG00000161011 E027 1273.0149493 8.521643e-05 5.598941e-03 1.755057e-02 5 179825146 179825226 81 + 2.984 3.024 0.134
ENSG00000161011 E028 11.3635592 3.060757e-03 3.155221e-01 4.582861e-01 5 179826068 179826291 224 + 1.093 0.988 -0.383
ENSG00000161011 E029 1985.6765451 8.988606e-05 2.533859e-03 8.865019e-03 5 179833032 179833227 196 + 3.180 3.216 0.122
ENSG00000161011 E030 913.8319667 8.268303e-05 7.421792e-01 8.311528e-01 5 179833228 179833246 19 + 2.863 2.874 0.036
ENSG00000161011 E031 53.0713888 4.373064e-04 2.890218e-01 4.296951e-01 5 179833247 179833386 140 + 1.593 1.661 0.232
ENSG00000161011 E032 2040.8020586 3.254848e-04 8.072663e-01 8.775703e-01 5 179833587 179833782 196 + 3.214 3.224 0.034
ENSG00000161011 E033 1052.5013906 5.231817e-04 6.234652e-01 7.400416e-01 5 179836436 179836470 35 + 2.923 2.938 0.052
ENSG00000161011 E034 4139.8612555 1.581041e-03 6.791070e-05 3.700805e-04 5 179836471 179839037 2567 + 3.470 3.545 0.247
ENSG00000161011 E035 3.4804414 1.072292e-02 1.648150e-04 8.127787e-04 5 179839038 179839857 820 + 0.953 0.396 -2.424
ENSG00000161011 E036 0.3332198 2.912910e-02 4.087868e-01   5 179839915 179839950 36 + 0.196 0.074 -1.615
ENSG00000161011 E037 1.2565790 4.794204e-02 3.091002e-01 4.513744e-01 5 179840270 179840416 147 + 0.433 0.243 -1.190