ENSG00000160991

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000488996 ENSG00000160991 HEK293_OSMI2_2hA HEK293_TMG_2hB ORAI2 protein_coding processed_transcript 9.786931 17.77563 8.396169 1.281686 0.2668006 -1.081192 2.806104 4.146721 3.590840 0.8495597 0.3834391 -0.2071123 0.3017000 0.2287000 0.4257667 0.1970667 0.013260418 0.0001608865 FALSE TRUE
MSTRG.30400.5 ENSG00000160991 HEK293_OSMI2_2hA HEK293_TMG_2hB ORAI2 protein_coding   9.786931 17.77563 8.396169 1.281686 0.2668006 -1.081192 5.915908 11.915635 3.857848 0.4594910 0.1559762 -1.6244632 0.5862917 0.6740667 0.4614333 -0.2126333 0.001932612 0.0001608865 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000160991 E001 0.3268771 0.0296687459 4.594314e-01   7 102433106 102433355 250 + 0.186 0.078 -1.435
ENSG00000160991 E002 0.6244956 0.0463605203 3.324074e-01 4.760627e-01 7 102433530 102433538 9 + 0.316 0.144 -1.440
ENSG00000160991 E003 1.8757761 0.0078000540 4.831071e-01 6.211827e-01 7 102433539 102433548 10 + 0.497 0.377 -0.629
ENSG00000160991 E004 3.6710132 0.0052817605 1.525363e-01 2.651297e-01 7 102433549 102433561 13 + 0.765 0.553 -0.906
ENSG00000160991 E005 3.8225287 0.0042921646 8.546737e-02 1.681591e-01 7 102433562 102433562 1 + 0.803 0.553 -1.058
ENSG00000160991 E006 5.5907965 0.0029153499 1.147444e-01 2.124593e-01 7 102433563 102433569 7 + 0.901 0.695 -0.815
ENSG00000160991 E007 9.3833215 0.0062231422 1.113697e-01 2.075568e-01 7 102433570 102433574 5 + 1.069 0.889 -0.666
ENSG00000160991 E008 9.3833215 0.0062231422 1.113697e-01 2.075568e-01 7 102433575 102433575 1 + 1.069 0.889 -0.666
ENSG00000160991 E009 50.3080546 0.0017018809 4.158198e-01 5.591577e-01 7 102433576 102433661 86 + 1.663 1.624 -0.133
ENSG00000160991 E010 1.7200193 0.0148591530 1.995886e-01 3.257137e-01 7 102436160 102436201 42 + 0.186 0.444 1.732
ENSG00000160991 E011 3.2959868 0.0161097433 5.899208e-01 7.129107e-01 7 102436202 102436224 23 + 0.497 0.599 0.472
ENSG00000160991 E012 40.2983261 0.0120483491 4.992941e-01 6.356148e-01 7 102436225 102436229 5 + 1.573 1.529 -0.150
ENSG00000160991 E013 73.7629591 0.0003976624 4.554984e-01 5.960189e-01 7 102436230 102436298 69 + 1.818 1.792 -0.089
ENSG00000160991 E014 63.5659941 0.0004011572 4.453835e-01 5.869266e-01 7 102436299 102436333 35 + 1.762 1.732 -0.099
ENSG00000160991 E015 148.3808048 0.0002469998 7.860523e-01 8.629867e-01 7 102438944 102439181 238 + 2.101 2.101 -0.001
ENSG00000160991 E016 271.2286258 0.0004361059 3.437362e-02 8.047328e-02 7 102446513 102447043 531 + 2.392 2.349 -0.144
ENSG00000160991 E017 709.2422881 0.0005314316 7.343775e-03 2.211256e-02 7 102447044 102448608 1565 + 2.801 2.770 -0.103
ENSG00000160991 E018 102.5928110 0.0002959988 4.773444e-01 6.159007e-01 7 102448609 102448779 171 + 1.956 1.937 -0.066
ENSG00000160991 E019 277.1326514 0.0032158913 5.725381e-02 1.219044e-01 7 102448780 102449332 553 + 2.314 2.390 0.253
ENSG00000160991 E020 193.1556289 0.0002914332 5.870217e-12 1.267778e-10 7 102449333 102449782 450 + 2.030 2.258 0.765
ENSG00000160991 E021 331.3434665 0.0052899019 1.527544e-02 4.111127e-02 7 102449783 102451419 1637 + 2.369 2.472 0.344
ENSG00000160991 E022 103.3144124 0.0005302787 1.456728e-05 9.319625e-05 7 102451420 102456825 5406 + 2.063 1.908 -0.521