ENSG00000160953

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000586067 ENSG00000160953 HEK293_OSMI2_2hA HEK293_TMG_2hB PWWP3A protein_coding processed_transcript 19.28214 23.27353 15.34423 1.789693 0.342625 -0.6006739 0.573349 1.8744457 0.000000 0.94066262 0.0000000 -7.5579964 0.02423333 0.07896667 0.00000000 -0.07896667 1.846134e-01 5.598542e-72 FALSE TRUE
ENST00000590695 ENSG00000160953 HEK293_OSMI2_2hA HEK293_TMG_2hB PWWP3A protein_coding retained_intron 19.28214 23.27353 15.34423 1.789693 0.342625 -0.6006739 2.352030 1.3037585 2.310726 0.06528346 0.1714150 0.8208760 0.12981667 0.05623333 0.15026667 0.09403333 1.287959e-08 5.598542e-72 FALSE FALSE
ENST00000590866 ENSG00000160953 HEK293_OSMI2_2hA HEK293_TMG_2hB PWWP3A protein_coding processed_transcript 19.28214 23.27353 15.34423 1.789693 0.342625 -0.6006739 1.198028 1.1552127 1.219569 0.18561145 0.1118207 0.0775596 0.06561250 0.05130000 0.07923333 0.02793333 3.315114e-01 5.598542e-72   FALSE
ENST00000591337 ENSG00000160953 HEK293_OSMI2_2hA HEK293_TMG_2hB PWWP3A protein_coding protein_coding 19.28214 23.27353 15.34423 1.789693 0.342625 -0.6006739 2.647296 2.4970450 2.104034 0.61876880 0.5846378 -0.2459895 0.13919167 0.10653333 0.13570000 0.02916667 8.408627e-01 5.598542e-72 FALSE TRUE
ENST00000591433 ENSG00000160953 HEK293_OSMI2_2hA HEK293_TMG_2hB PWWP3A protein_coding nonsense_mediated_decay 19.28214 23.27353 15.34423 1.789693 0.342625 -0.6006739 1.094612 0.5997492 1.441623 0.07718590 0.1907568 1.2513789 0.06059167 0.02583333 0.09463333 0.06880000 6.132071e-05 5.598542e-72 FALSE TRUE
ENST00000652273 ENSG00000160953 HEK293_OSMI2_2hA HEK293_TMG_2hB PWWP3A protein_coding protein_coding 19.28214 23.27353 15.34423 1.789693 0.342625 -0.6006739 3.735776 8.8481654 0.000000 0.84050451 0.0000000 -9.7908641 0.17284167 0.37930000 0.00000000 -0.37930000 5.598542e-72 5.598542e-72 FALSE TRUE
MSTRG.16050.3 ENSG00000160953 HEK293_OSMI2_2hA HEK293_TMG_2hB PWWP3A protein_coding   19.28214 23.27353 15.34423 1.789693 0.342625 -0.6006739 3.838586 3.6400376 4.990093 0.80241418 0.3149924 0.4540435 0.20733333 0.16240000 0.32646667 0.16406667 9.309735e-02 5.598542e-72 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000160953 E001 0.3697384 0.0274424043 4.066580e-01 5.502906e-01 19 1354711 1354921 211 + 0.000 0.175 9.356
ENSG00000160953 E002 0.2987644 0.0290785164 8.595848e-02   19 1354922 1354953 32 + 0.268 0.000 -13.196
ENSG00000160953 E003 0.4470576 0.0227809547 2.862051e-01 4.266088e-01 19 1354954 1354958 5 + 0.268 0.096 -1.788
ENSG00000160953 E004 1.4100585 0.0805846166 9.776411e-02 1.870914e-01 19 1354959 1354971 13 + 0.552 0.241 -1.792
ENSG00000160953 E005 10.7619668 0.0143326254 6.272964e-04 2.633211e-03 19 1354972 1355020 49 + 1.258 0.886 -1.352
ENSG00000160953 E006 10.9511507 0.0474956185 5.207608e-01 6.547119e-01 19 1355021 1355135 115 + 1.099 1.014 -0.307
ENSG00000160953 E007 0.4355181 0.6899287493 1.655897e-01 2.824743e-01 19 1356290 1356313 24 + 0.356 0.000 -12.520
ENSG00000160953 E008 0.5117906 0.2496429435 3.588648e-01 5.032404e-01 19 1356314 1356316 3 + 0.268 0.098 -1.758
ENSG00000160953 E009 0.5117906 0.2496429435 3.588648e-01 5.032404e-01 19 1356317 1356322 6 + 0.268 0.098 -1.758
ENSG00000160953 E010 0.5117906 0.2496429435 3.588648e-01 5.032404e-01 19 1356323 1356323 1 + 0.268 0.098 -1.758
ENSG00000160953 E011 33.3494330 0.0038051174 7.306913e-04 3.009485e-03 19 1356324 1356363 40 + 1.632 1.421 -0.723
ENSG00000160953 E012 38.4256215 0.0006693405 1.026920e-04 5.344840e-04 19 1356364 1356389 26 + 1.689 1.481 -0.709
ENSG00000160953 E013 52.0809859 0.0005513972 9.446521e-05 4.960680e-04 19 1356390 1356449 60 + 1.802 1.623 -0.608
ENSG00000160953 E014 74.4821322 0.0075451898 3.712121e-03 1.233231e-02 19 1357009 1357094 86 + 1.941 1.790 -0.509
ENSG00000160953 E015 81.8897676 0.0003787012 3.601655e-22 2.915909e-20 19 1357095 1358393 1299 + 2.097 1.735 -1.217
ENSG00000160953 E016 74.5022554 0.0023250122 1.620678e-03 6.027102e-03 19 1358394 1358464 71 + 1.930 1.799 -0.444
ENSG00000160953 E017 28.6532122 0.0009108867 3.858786e-07 3.488597e-06 19 1358588 1358693 106 + 1.628 1.314 -1.082
ENSG00000160953 E018 12.7778314 0.0014259523 4.393445e-04 1.925856e-03 19 1358694 1358700 7 + 1.297 0.986 -1.116
ENSG00000160953 E019 40.4258042 0.0245087772 2.796153e-02 6.793931e-02 19 1358701 1359894 1194 + 1.708 1.526 -0.621
ENSG00000160953 E020 256.8628524 0.0002365270 2.473033e-09 3.408575e-08 19 1360136 1360631 496 + 2.456 2.337 -0.397
ENSG00000160953 E021 211.5315458 0.0012538980 5.160270e-02 1.120045e-01 19 1360632 1361032 401 + 2.322 2.281 -0.139
ENSG00000160953 E022 1.8144532 0.1736238167 4.461971e-01 5.876943e-01 19 1361896 1362249 354 + 0.497 0.360 -0.729
ENSG00000160953 E023 105.2208059 0.0003629621 5.356843e-01 6.674852e-01 19 1362250 1362351 102 + 1.966 2.003 0.125
ENSG00000160953 E024 97.6458426 0.0016175412 5.255258e-01 6.588749e-01 19 1364509 1364579 71 + 1.930 1.972 0.141
ENSG00000160953 E025 104.9158169 0.0026943517 8.674004e-01 9.187380e-01 19 1366305 1366381 77 + 1.978 2.001 0.078
ENSG00000160953 E026 82.9564364 0.0003801703 5.099748e-01 6.452365e-01 19 1367160 1367220 61 + 1.865 1.906 0.140
ENSG00000160953 E027 4.2093937 0.0426582817 6.038384e-01 7.245165e-01 19 1369104 1369264 161 + 0.722 0.655 -0.281
ENSG00000160953 E028 119.9747411 0.0003599765 8.893612e-01 9.332898e-01 19 1369265 1369340 76 + 2.040 2.059 0.065
ENSG00000160953 E029 103.8004495 0.0026913427 7.568504e-01 8.418763e-01 19 1369596 1369646 51 + 1.989 1.991 0.007
ENSG00000160953 E030 278.0267177 0.0001810383 1.123006e-03 4.380367e-03 19 1370642 1371078 437 + 2.353 2.441 0.293
ENSG00000160953 E031 7.9857556 0.0168446496 3.848387e-02 8.825356e-02 19 1371079 1371233 155 + 1.083 0.840 -0.906
ENSG00000160953 E032 5.4802736 0.0034778184 2.702358e-03 9.381423e-03 19 1371234 1371322 89 + 0.999 0.626 -1.477
ENSG00000160953 E033 4.9498325 0.0074782124 3.795565e-02 8.726648e-02 19 1371323 1371386 64 + 0.917 0.650 -1.068
ENSG00000160953 E034 3.3568231 0.0045793279 2.792597e-01 4.189891e-01 19 1371387 1371390 4 + 0.722 0.571 -0.650
ENSG00000160953 E035 7.3843100 0.0027532900 5.037455e-01 6.396973e-01 19 1371391 1371479 89 + 0.939 0.872 -0.255
ENSG00000160953 E036 23.7295254 0.0008026287 9.822450e-05 5.137019e-04 19 1372099 1373071 973 + 1.519 1.258 -0.906
ENSG00000160953 E037 134.8695011 0.0003431650 9.982150e-03 2.871987e-02 19 1373072 1373160 89 + 2.033 2.132 0.334
ENSG00000160953 E038 11.8224787 0.0017466582 8.981589e-02 1.748410e-01 19 1373507 1374049 543 + 1.179 1.028 -0.545
ENSG00000160953 E039 8.6108455 0.0069837165 5.356170e-03 1.690001e-02 19 1374050 1374371 322 + 1.141 0.841 -1.111
ENSG00000160953 E040 12.5206820 0.0014235237 1.457143e-01 2.558187e-01 19 1376145 1376518 374 + 0.980 1.143 0.593
ENSG00000160953 E041 748.8572175 0.0009419246 1.982045e-39 6.634141e-37 19 1376519 1378431 1913 + 2.665 2.910 0.817