ENSG00000160888

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000292433 ENSG00000160888 HEK293_OSMI2_2hA HEK293_TMG_2hB IER2 protein_coding protein_coding 23.71415 39.03023 12.65483 2.371925 0.2905327 -1.624134 15.901941 26.819134 9.837138 2.853893 0.34863644 -1.4460241 0.68777083 0.6884000 0.7768667 0.08846667 5.089328e-01 3.270195e-09 FALSE TRUE
ENST00000587885 ENSG00000160888 HEK293_OSMI2_2hA HEK293_TMG_2hB IER2 protein_coding protein_coding 23.71415 39.03023 12.65483 2.371925 0.2905327 -1.624134 3.159488 6.828804 0.000000 2.671142 0.00000000 -9.4176002 0.09137083 0.1738667 0.0000000 -0.17386667 3.270195e-09 3.270195e-09 FALSE TRUE
ENST00000588173 ENSG00000160888 HEK293_OSMI2_2hA HEK293_TMG_2hB IER2 protein_coding protein_coding 23.71415 39.03023 12.65483 2.371925 0.2905327 -1.624134 4.652722 5.382293 2.817689 0.395672 0.07048412 -0.9312754 0.22085000 0.1377000 0.2231333 0.08543333 1.571454e-04 3.270195e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000160888 E001 1.984417 0.0075011089 4.892952e-02 1.072567e-01 19 13150411 13150414 4 + 0.651 0.322 -1.662
ENSG00000160888 E002 20.542554 0.0022300428 9.381541e-05 4.928880e-04 19 13150415 13150552 138 + 1.459 1.152 -1.075
ENSG00000160888 E003 104.599349 0.0002742445 3.742094e-06 2.742229e-05 19 13151975 13152943 969 + 2.052 1.890 -0.543
ENSG00000160888 E004 129.852517 0.0022315081 1.493251e-02 4.033296e-02 19 13152944 13153096 153 + 2.086 2.004 -0.277
ENSG00000160888 E005 1454.344880 0.0007499024 1.170969e-09 1.713421e-08 19 13153097 13154911 1815 + 3.032 3.079 0.157