ENSG00000160685

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000368426 ENSG00000160685 HEK293_OSMI2_2hA HEK293_TMG_2hB ZBTB7B protein_coding protein_coding 6.46441 9.760973 3.408978 0.6794995 0.1821829 -1.514937 1.9356442 3.258485 0.4475013 0.3293158 0.18717724 -2.8367743 0.28537917 0.3323333 0.1291667 -0.203166667 0.02874505 0.02874505 FALSE TRUE
ENST00000417934 ENSG00000160685 HEK293_OSMI2_2hA HEK293_TMG_2hB ZBTB7B protein_coding protein_coding 6.46441 9.760973 3.408978 0.6794995 0.1821829 -1.514937 0.5525628 1.032831 0.3777069 0.4312978 0.09010189 -1.4274666 0.08629583 0.1015667 0.1090000 0.007433333 0.91341121 0.02874505 FALSE TRUE
ENST00000535420 ENSG00000160685 HEK293_OSMI2_2hA HEK293_TMG_2hB ZBTB7B protein_coding protein_coding 6.46441 9.760973 3.408978 0.6794995 0.1821829 -1.514937 3.0845664 4.310964 2.3110544 0.1695581 0.29489519 -0.8965728 0.50260833 0.4487000 0.6811000 0.232400000 0.16083784 0.02874505 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000160685 E001 1.621006 0.2471567154 0.90846372 0.94586482 1 155002630 155002656 27 + 0.326 0.371 0.269
ENSG00000160685 E002 6.202755 0.0344518079 0.61979018 0.73710194 1 155002657 155002686 30 + 0.692 0.802 0.443
ENSG00000160685 E003 7.503416 0.0206253659 0.56175008 0.68948855 1 155002687 155002801 115 + 0.888 0.828 -0.231
ENSG00000160685 E004 18.731117 0.0010528925 0.08351118 0.16504733 1 155002802 155002842 41 + 1.302 1.176 -0.447
ENSG00000160685 E005 25.235770 0.0078344921 0.23283578 0.36579735 1 155002843 155002844 2 + 1.390 1.314 -0.266
ENSG00000160685 E006 42.198594 0.0047234594 0.03097994 0.07387573 1 155002845 155002943 99 + 1.634 1.518 -0.396
ENSG00000160685 E007 9.646720 0.0575626368 0.74125493 0.83048099 1 155010258 155010397 140 + 0.948 0.936 -0.042
ENSG00000160685 E008 3.334018 0.0562760360 0.58617376 0.70978147 1 155010916 155011015 100 + 0.640 0.559 -0.359
ENSG00000160685 E009 3.936389 0.0042330529 0.79021965 0.86580956 1 155014015 155014093 79 + 0.580 0.643 0.278
ENSG00000160685 E010 3.264764 0.0434067951 0.20026748 0.32655443 1 155014448 155014654 207 + 0.327 0.607 1.441
ENSG00000160685 E011 252.775726 0.0001992914 0.07714417 0.15491113 1 155014655 155015814 1160 + 2.326 2.313 -0.045
ENSG00000160685 E012 445.812314 0.0062010355 0.02851541 0.06904689 1 155016220 155018523 2304 + 2.500 2.583 0.277