ENSG00000160460

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000392023 ENSG00000160460 HEK293_OSMI2_2hA HEK293_TMG_2hB SPTBN4 protein_coding protein_coding 2.502977 4.357491 1.966938 0.1144845 0.1000117 -1.143537 0.64650360 1.24530167 0.49142062 0.105014638 0.06653947 -1.3239409 0.25103750 0.28606667 0.25433333 -0.03173333 0.862241423 0.00549548 FALSE TRUE
ENST00000593816 ENSG00000160460 HEK293_OSMI2_2hA HEK293_TMG_2hB SPTBN4 protein_coding processed_transcript 2.502977 4.357491 1.966938 0.1144845 0.1000117 -1.143537 0.10262562 0.13507360 0.04914541 0.046115805 0.02457531 -1.2944469 0.06645833 0.03073333 0.02633333 -0.00440000 0.938788751 0.00549548   FALSE
ENST00000595690 ENSG00000160460 HEK293_OSMI2_2hA HEK293_TMG_2hB SPTBN4 protein_coding retained_intron 2.502977 4.357491 1.966938 0.1144845 0.1000117 -1.143537 0.09502192 0.05935586 0.24471825 0.034818870 0.05855788 1.8768127 0.05062500 0.01386667 0.12720000 0.11333333 0.005495480 0.00549548   FALSE
ENST00000596900 ENSG00000160460 HEK293_OSMI2_2hA HEK293_TMG_2hB SPTBN4 protein_coding retained_intron 2.502977 4.357491 1.966938 0.1144845 0.1000117 -1.143537 0.15655488 0.12748180 0.14620701 0.050438737 0.05690883 0.1842185 0.07237917 0.02960000 0.07303333 0.04343333 0.541181347 0.00549548 FALSE TRUE
ENST00000597389 ENSG00000160460 HEK293_OSMI2_2hA HEK293_TMG_2hB SPTBN4 protein_coding nonsense_mediated_decay 2.502977 4.357491 1.966938 0.1144845 0.1000117 -1.143537 0.14201213 0.15800914 0.24534826 0.006171431 0.01711389 0.6039265 0.06462500 0.03636667 0.12603333 0.08966667 0.008789307 0.00549548 FALSE TRUE
ENST00000599926 ENSG00000160460 HEK293_OSMI2_2hA HEK293_TMG_2hB SPTBN4 protein_coding processed_transcript 2.502977 4.357491 1.966938 0.1144845 0.1000117 -1.143537 0.75093324 1.37485115 0.33267363 0.151499776 0.17648477 -2.0148239 0.27372500 0.31490000 0.16223333 -0.15266667 0.532790864 0.00549548   FALSE
MSTRG.17165.6 ENSG00000160460 HEK293_OSMI2_2hA HEK293_TMG_2hB SPTBN4 protein_coding   2.502977 4.357491 1.966938 0.1144845 0.1000117 -1.143537 0.49835910 1.19144586 0.43445198 0.079166450 0.12654324 -1.4346722 0.17322917 0.27336667 0.21920000 -0.05416667 0.705881220 0.00549548 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000160460 E001 0.2214452 0.036982196 9.399763e-01   19 40466241 40466311 71 + 0.000 0.093 9.879
ENSG00000160460 E002 0.0000000       19 40467001 40467305 305 +      
ENSG00000160460 E003 0.0000000       19 40472607 40472790 184 +      
ENSG00000160460 E004 0.0000000       19 40487697 40487848 152 +      
ENSG00000160460 E005 0.1482932 0.044135179 9.405830e-01   19 40490075 40490248 174 + 0.000 0.092 12.113
ENSG00000160460 E006 0.2214452 0.036982196 9.399763e-01   19 40492963 40493054 92 + 0.000 0.093 12.127
ENSG00000160460 E007 0.2214452 0.036982196 9.399763e-01   19 40494897 40494977 81 + 0.000 0.093 12.127
ENSG00000160460 E008 0.0000000       19 40497429 40497488 60 +      
ENSG00000160460 E009 0.2214452 0.036982196 9.399763e-01   19 40497489 40497604 116 + 0.000 0.093 12.127
ENSG00000160460 E010 0.2214452 0.036982196 9.399763e-01   19 40501921 40502033 113 + 0.000 0.093 12.127
ENSG00000160460 E011 0.1817044 0.040687716 9.401330e-01   19 40502128 40502315 188 + 0.000 0.093 12.120
ENSG00000160460 E012 0.1817044 0.040687716 9.401330e-01   19 40502390 40502507 118 + 0.000 0.093 12.120
ENSG00000160460 E013 0.0000000       19 40502775 40502933 159 +      
ENSG00000160460 E014 0.3729606 0.025646026 6.296292e-01 7.449637e-01 19 40503830 40504132 303 + 0.160 0.093 -0.909
ENSG00000160460 E015 0.3729606 0.025646026 6.296292e-01 7.449637e-01 19 40506236 40506386 151 + 0.160 0.093 -0.909
ENSG00000160460 E016 0.0000000       19 40512606 40512613 8 +      
ENSG00000160460 E017 0.7761102 0.018178633 7.015004e-01 8.006415e-01 19 40512614 40513554 941 + 0.160 0.234 0.677
ENSG00000160460 E018 0.0000000       19 40515311 40515448 138 +      
ENSG00000160460 E019 1.3306604 0.144594564 5.403695e-02 1.163284e-01 19 40519401 40520057 657 + 0.000 0.429 14.396
ENSG00000160460 E020 1.4789536 0.143373298 3.883336e-02 8.887139e-02 19 40520058 40520151 94 + 0.000 0.464 14.538
ENSG00000160460 E021 8.2721039 0.013508716 4.647324e-01 6.044536e-01 19 40523437 40523639 203 + 0.860 0.968 0.407
ENSG00000160460 E022 0.2214452 0.036982196 9.399763e-01   19 40524544 40524629 86 + 0.000 0.093 12.127
ENSG00000160460 E023 9.5637917 0.003156029 5.610935e-01 6.889769e-01 19 40529041 40529131 91 + 0.934 1.011 0.287
ENSG00000160460 E024 1.8037554 0.013236238 7.853374e-01 8.623917e-01 19 40530488 40530525 38 + 0.369 0.426 0.314
ENSG00000160460 E025 2.3970299 0.006382475 8.961606e-01 9.376463e-01 19 40530526 40530590 65 + 0.509 0.496 -0.063
ENSG00000160460 E026 0.1472490 0.041531990 2.553061e-01   19 40530766 40530856 91 + 0.160 0.000 -14.246
ENSG00000160460 E027 21.8139738 0.033251937 1.108788e-01 2.068402e-01 19 40532625 40532771 147 + 1.184 1.371 0.656
ENSG00000160460 E028 22.2382330 0.001072101 4.177918e-01 5.611085e-01 19 40534080 40534343 264 + 1.275 1.350 0.259
ENSG00000160460 E029 0.0000000       19 40534344 40534856 513 +      
ENSG00000160460 E030 1.9155300 0.034598238 8.838808e-01 9.297524e-01 19 40538613 40538735 123 + 0.445 0.426 -0.099
ENSG00000160460 E031 12.0289808 0.001747233 7.702028e-01 8.516148e-01 19 40549189 40549413 225 + 1.052 1.093 0.150
ENSG00000160460 E032 5.3730399 0.034876708 3.202511e-01 4.633538e-01 19 40550238 40550327 90 + 0.861 0.723 -0.548
ENSG00000160460 E033 12.4476645 0.001707016 1.191932e-01 2.187863e-01 19 40554147 40554425 279 + 1.184 1.049 -0.485
ENSG00000160460 E034 11.9133923 0.004344302 7.747636e-01 8.548336e-01 19 40554516 40554646 131 + 1.052 1.093 0.149
ENSG00000160460 E035 3.8832737 0.015460476 4.495810e-01 5.907817e-01 19 40554647 40555302 656 + 0.738 0.635 -0.431
ENSG00000160460 E036 18.6824775 0.010328765 8.176355e-01 8.847001e-01 19 40556084 40556288 205 + 1.232 1.265 0.116
ENSG00000160460 E037 26.7717576 0.002089078 4.211535e-01 5.642793e-01 19 40557023 40557403 381 + 1.359 1.429 0.242
ENSG00000160460 E038 17.6721622 0.019139689 5.993431e-01 7.207617e-01 19 40560159 40560403 245 + 1.184 1.257 0.258
ENSG00000160460 E039 4.1307527 0.003965951 1.582458e-01 2.727786e-01 19 40560404 40560752 349 + 0.509 0.741 1.014
ENSG00000160460 E040 7.5466395 0.002681765 4.965146e-03 1.583316e-02 19 40565423 40565561 139 + 1.085 0.777 -1.163
ENSG00000160460 E041 5.1149769 0.041412727 6.009942e-02 1.267735e-01 19 40565661 40565745 85 + 0.935 0.656 -1.105
ENSG00000160460 E042 7.0051360 0.002482290 4.629000e-02 1.025601e-01 19 40566163 40566359 197 + 1.016 0.794 -0.845
ENSG00000160460 E043 5.5941140 0.003074803 3.385641e-06 2.505663e-05 19 40567663 40568282 620 + 1.101 0.528 -2.290
ENSG00000160460 E044 1.4747028 0.010627438 5.232118e-01 6.569389e-01 19 40569657 40569726 70 + 0.445 0.340 -0.590
ENSG00000160460 E045 2.5681277 0.024369869 8.689183e-02 1.704202e-01 19 40570436 40570728 293 + 0.700 0.425 -1.273
ENSG00000160460 E046 0.4815130 0.022078365 1.000000e+00 1.000000e+00 19 40571039 40571045 7 + 0.160 0.169 0.087
ENSG00000160460 E047 4.4137228 0.004409663 2.780440e-01 4.176714e-01 19 40571046 40571093 48 + 0.566 0.741 0.753
ENSG00000160460 E048 0.1472490 0.041531990 2.553061e-01   19 40571544 40571639 96 + 0.160 0.000 -14.246
ENSG00000160460 E049 1.9563045 0.007427181 8.912883e-02 1.737982e-01 19 40571640 40572018 379 + 0.615 0.340 -1.397
ENSG00000160460 E050 9.5941587 0.057477356 5.333559e-01 6.655601e-01 19 40572019 40572192 174 + 1.034 0.957 -0.283
ENSG00000160460 E051 0.2214452 0.036982196 9.399763e-01   19 40572193 40572337 145 + 0.000 0.093 12.127
ENSG00000160460 E052 7.6451540 0.003097176 7.526349e-01 8.387677e-01 19 40572338 40572380 43 + 0.860 0.910 0.190
ENSG00000160460 E053 1.5770116 0.009132097 4.413857e-01 5.830168e-01 19 40572381 40572632 252 + 0.277 0.425 0.897
ENSG00000160460 E054 41.3655826 0.010345925 1.941581e-01 3.189892e-01 19 40575411 40576464 1054 + 1.520 1.624 0.353