ENSG00000160229

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000344519 ENSG00000160229 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF66 protein_coding protein_coding 0.568474 0.5075775 0.7375754 0.08758633 0.09475517 0.5304444 0.34154112 0.09279537 0.5104040 0.032501093 0.04507498 2.3398567 0.60352083 0.18233333 0.7149333 0.53260000 0.009999184 0.009999184 FALSE TRUE
ENST00000360204 ENSG00000160229 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF66 protein_coding protein_coding 0.568474 0.5075775 0.7375754 0.08758633 0.09475517 0.5304444 0.03018042 0.03799428 0.0000000 0.004646723 0.00000000 -2.2628626 0.05159583 0.07903333 0.0000000 -0.07903333 0.193806843 0.009999184   FALSE
ENST00000594534 ENSG00000160229 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF66 protein_coding protein_coding 0.568474 0.5075775 0.7375754 0.08758633 0.09475517 0.5304444 0.19675243 0.37678780 0.2271714 0.072854554 0.10447755 -0.7056119 0.34490000 0.73866667 0.2850667 -0.45360000 0.026840792 0.009999184 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000160229 E001 0.3666179 0.028999279 5.494415e-01 6.791685e-01 19 20776304 20776309 6 + 0.082 0.180 1.310
ENSG00000160229 E002 3.9279474 0.017456269 1.593209e-02 4.258003e-02 19 20776310 20776450 141 + 0.515 0.884 1.549
ENSG00000160229 E003 0.4782907 0.060895742 1.613655e-02 4.303482e-02 19 20776451 20776481 31 + 0.000 0.403 13.121
ENSG00000160229 E004 3.9364682 0.004557949 1.031272e-01 1.952386e-01 19 20792512 20792638 127 + 0.567 0.822 1.067
ENSG00000160229 E005 2.9099649 0.008701840 7.625934e-03 2.283860e-02 19 20793783 20793878 96 + 0.351 0.788 2.049
ENSG00000160229 E006 0.4428904 0.252703572 8.722554e-02 1.709349e-01 19 20793879 20794597 719 + 0.000 0.313 12.461
ENSG00000160229 E007 3.1364075 0.038408154 4.858663e-03 1.553885e-02 19 20799001 20799393 393 + 0.389 0.853 2.080
ENSG00000160229 E008 20.8327036 0.001721379 7.999884e-13 1.995726e-11 19 20805827 20809995 4169 + 1.405 0.987 -1.487