ENSG00000160221

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000291577 ENSG00000160221 HEK293_OSMI2_2hA HEK293_TMG_2hB GATD3 protein_coding protein_coding 68.91966 122.5388 42.68359 2.478262 2.20822 -1.521265 21.976430 42.275212 10.578339 4.3561202 1.5806098 -1.9976770 0.30199583 0.34596667 0.24603333 -0.09993333 2.567625e-01 1.196779e-15 FALSE TRUE
ENST00000348499 ENSG00000160221 HEK293_OSMI2_2hA HEK293_TMG_2hB GATD3 protein_coding protein_coding 68.91966 122.5388 42.68359 2.478262 2.20822 -1.521265 9.214269 19.407918 4.800415 0.7653607 0.8254238 -2.0131552 0.11858750 0.15830000 0.11490000 -0.04340000 4.220583e-01 1.196779e-15 FALSE TRUE
ENST00000427803 ENSG00000160221 HEK293_OSMI2_2hA HEK293_TMG_2hB GATD3 protein_coding protein_coding 68.91966 122.5388 42.68359 2.478262 2.20822 -1.521265 9.552859 17.095170 5.404687 2.1384023 0.6263847 -1.6594827 0.13505000 0.13946667 0.12626667 -0.01320000 8.643218e-01 1.196779e-15 FALSE TRUE
ENST00000488392 ENSG00000160221 HEK293_OSMI2_2hA HEK293_TMG_2hB GATD3 protein_coding retained_intron 68.91966 122.5388 42.68359 2.478262 2.20822 -1.521265 3.338832 3.472731 2.306834 0.5692621 0.4481850 -0.5880647 0.05632500 0.02816667 0.05346667 0.02530000 7.371746e-02 1.196779e-15 FALSE TRUE
ENST00000493883 ENSG00000160221 HEK293_OSMI2_2hA HEK293_TMG_2hB GATD3 protein_coding processed_transcript 68.91966 122.5388 42.68359 2.478262 2.20822 -1.521265 1.977738 6.619778 0.000000 1.7128096 0.0000000 -9.3728167 0.02182500 0.05350000 0.00000000 -0.05350000 1.196779e-15 1.196779e-15 FALSE TRUE
ENST00000495007 ENSG00000160221 HEK293_OSMI2_2hA HEK293_TMG_2hB GATD3 protein_coding retained_intron 68.91966 122.5388 42.68359 2.478262 2.20822 -1.521265 4.191941 6.011838 3.384596 0.8594117 0.2674720 -0.8269639 0.06667083 0.04893333 0.07920000 0.03026667 7.014995e-02 1.196779e-15 FALSE TRUE
MSTRG.21426.5 ENSG00000160221 HEK293_OSMI2_2hA HEK293_TMG_2hB GATD3 protein_coding   68.91966 122.5388 42.68359 2.478262 2.20822 -1.521265 9.950504 16.642212 7.936721 1.3561892 1.5258869 -1.0672822 0.15587500 0.13563333 0.18563333 0.05000000 5.424952e-01 1.196779e-15 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000160221 E001 0.1515154 0.0428592299 0.10910614   21 44133053 44133295 243 + 0.214 0.000 -11.861
ENSG00000160221 E002 0.9974562 0.0130506316 0.10829152 0.20300522 21 44133610 44133615 6 + 0.464 0.176 -1.935
ENSG00000160221 E003 0.9974562 0.0130506316 0.10829152 0.20300522 21 44133616 44133616 1 + 0.464 0.176 -1.935
ENSG00000160221 E004 3.4267383 0.0069344514 0.13084826 0.23532862 21 44133617 44133617 1 + 0.737 0.500 -1.042
ENSG00000160221 E005 12.1674819 0.0066363566 0.05289821 0.11430699 21 44133618 44133638 21 + 1.176 0.976 -0.727
ENSG00000160221 E006 16.3490857 0.0029487238 0.54559190 0.67590061 21 44133639 44133641 3 + 1.176 1.129 -0.169
ENSG00000160221 E007 51.1677228 0.0013272898 0.86335272 0.91585380 21 44133642 44133673 32 + 1.579 1.604 0.085
ENSG00000160221 E008 57.1023221 0.0005782843 0.42763655 0.57050027 21 44133674 44133679 6 + 1.671 1.640 -0.105
ENSG00000160221 E009 67.8441638 0.0025491422 0.45642851 0.59685866 21 44133680 44133682 3 + 1.741 1.714 -0.092
ENSG00000160221 E010 147.4124609 0.0021398816 0.20235304 0.32916185 21 44133683 44133778 96 + 2.079 2.046 -0.113
ENSG00000160221 E011 93.9908072 0.0018737902 0.78667796 0.86338335 21 44133779 44133838 60 + 1.836 1.863 0.093
ENSG00000160221 E012 1.7671220 0.0082694640 0.98680595 0.99581017 21 44133839 44134101 263 + 0.357 0.368 0.065
ENSG00000160221 E013 66.8632961 0.0008217012 0.78946259 0.86525593 21 44134102 44134154 53 + 1.715 1.715 0.000
ENSG00000160221 E014 32.4006533 0.0022815708 0.01401978 0.03827041 21 44136060 44136074 15 + 1.533 1.368 -0.566
ENSG00000160221 E015 37.0903333 0.0068703312 0.16524255 0.28198881 21 44136075 44136106 32 + 1.533 1.442 -0.312
ENSG00000160221 E016 20.7719404 0.0178954366 0.23990701 0.37402820 21 44136107 44136118 12 + 1.303 1.196 -0.376
ENSG00000160221 E017 21.0288731 0.0199513702 0.26867568 0.40715607 21 44136119 44136150 32 + 1.303 1.202 -0.356
ENSG00000160221 E018 15.6059188 0.0012123432 0.30159173 0.44341385 21 44136151 44136173 23 + 1.176 1.085 -0.328
ENSG00000160221 E019 0.0000000       21 44136874 44136982 109 +      
ENSG00000160221 E020 0.0000000       21 44136983 44137027 45 +      
ENSG00000160221 E021 14.9762260 0.0217930442 0.37592128 0.52030884 21 44137177 44137296 120 + 1.158 1.067 -0.327
ENSG00000160221 E022 0.3289534 0.0281200196 0.31785634   21 44137297 44137575 279 + 0.214 0.067 -1.935
ENSG00000160221 E023 9.4710544 0.0021606699 0.80957300 0.87910936 21 44140250 44140342 93 + 0.867 0.912 0.170
ENSG00000160221 E024 4.0514599 0.0042046256 0.25024428 0.38622556 21 44140343 44140604 262 + 0.737 0.564 -0.742
ENSG00000160221 E025 15.7047847 0.0014870793 0.01383282 0.03784677 21 44140942 44141163 222 + 0.828 1.146 1.181
ENSG00000160221 E026 14.4546502 0.0015666938 0.01424783 0.03878841 21 44141164 44141417 254 + 0.785 1.114 1.236
ENSG00000160221 E027 10.1181725 0.0017143675 0.00565155 0.01768970 21 44143205 44143361 157 + 0.550 0.992 1.792
ENSG00000160221 E028 17.0426747 0.0019764722 0.13531174 0.24155142 21 44144821 44144916 96 + 0.996 1.175 0.649
ENSG00000160221 E029 124.9852449 0.0170472932 0.12980401 0.23388797 21 44144917 44145570 654 + 1.881 2.006 0.420
ENSG00000160221 E030 130.5854505 0.0143845890 0.07104958 0.14508672 21 44145571 44145723 153 + 1.888 2.027 0.465
ENSG00000160221 E031 0.1472490 0.0420257657 0.10956450   21 44162582 44162823 242 + 0.214 0.000 -14.335
ENSG00000160221 E032 0.3299976 0.0277225971 1.00000000   21 44193624 44194535 912 + 0.000 0.125 11.888
ENSG00000160221 E033 0.1451727 0.0430799312 0.10924708   21 44197171 44197892 722 + 0.214 0.000 -14.326
ENSG00000160221 E034 0.0000000       21 44202050 44203064 1015 +      
ENSG00000160221 E035 0.5149242 0.2246835478 0.58269913 0.70699004 21 44207913 44208219 307 + 0.214 0.125 -0.931
ENSG00000160221 E036 0.1515154 0.0428592299 0.10910614   21 44208469 44210114 1646 + 0.214 0.000 -14.329