ENSG00000159840

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000322764 ENSG00000159840 HEK293_OSMI2_2hA HEK293_TMG_2hB ZYX protein_coding protein_coding 65.90093 130.4918 46.93884 6.572359 0.4926138 -1.474908 40.123990 87.16802 25.623233 4.498311 1.2764835 -1.7659491 0.61025000 0.6680000 0.54616667 -0.1218333 0.014148119 0.0003795938 FALSE TRUE
ENST00000354434 ENSG00000159840 HEK293_OSMI2_2hA HEK293_TMG_2hB ZYX protein_coding protein_coding 65.90093 130.4918 46.93884 6.572359 0.4926138 -1.474908 7.138013 21.71204 3.109672 2.393498 0.3773445 -2.7996933 0.08796667 0.1688667 0.06616667 -0.1027000 0.001888072 0.0003795938 FALSE TRUE
MSTRG.30855.2 ENSG00000159840 HEK293_OSMI2_2hA HEK293_TMG_2hB ZYX protein_coding   65.90093 130.4918 46.93884 6.572359 0.4926138 -1.474908 15.400683 18.79902 15.333589 4.954447 0.9734391 -0.2937887 0.22845417 0.1411000 0.32656667 0.1854667 0.017352102 0.0003795938 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000159840 E001 3.737299 0.1825848242 8.134725e-02 1.616624e-01 7 143379609 143380987 1379 + 0.894 0.484 -1.736
ENSG00000159840 E002 5.523114 0.0587104322 8.465026e-01 9.046198e-01 7 143381225 143381294 70 + 0.743 0.719 -0.095
ENSG00000159840 E003 31.292831 0.0788373341 5.567759e-05 3.100324e-04 7 143381295 143381344 50 + 1.794 1.222 -1.966
ENSG00000159840 E004 39.968679 0.1140247725 6.528002e-05 3.572759e-04 7 143381345 143381359 15 + 1.935 1.285 -2.219
ENSG00000159840 E005 47.597800 0.1430572470 1.575297e-04 7.806924e-04 7 143381360 143381360 1 + 2.018 1.349 -2.275
ENSG00000159840 E006 124.735981 0.0353061475 2.792483e-05 1.672708e-04 7 143381361 143381409 49 + 2.285 1.895 -1.306
ENSG00000159840 E007 7.500364 0.0071236654 1.541258e-02 4.142831e-02 7 143381410 143381556 147 + 1.070 0.768 -1.145
ENSG00000159840 E008 60.010592 0.0216006284 8.302692e-07 7.016639e-06 7 143381557 143381559 3 + 1.983 1.571 -1.393
ENSG00000159840 E009 134.200365 0.0193397980 2.113603e-06 1.635484e-05 7 143381560 143381574 15 + 2.290 1.943 -1.162
ENSG00000159840 E010 411.520500 0.0071061491 1.196969e-03 4.630068e-03 7 143381575 143381779 205 + 2.634 2.494 -0.465
ENSG00000159840 E011 2.266725 0.0116692378 1.275243e-05 8.266869e-05 7 143381907 143382027 121 + 0.892 0.193 -3.600
ENSG00000159840 E012 2.113031 0.0192732409 2.769060e-03 9.580329e-03 7 143382028 143382091 64 + 0.785 0.286 -2.452
ENSG00000159840 E013 529.712780 0.0011836699 8.233300e-02 1.631918e-01 7 143382248 143382447 200 + 2.667 2.627 -0.135
ENSG00000159840 E014 9.277719 0.0018738152 1.609373e-03 5.991175e-03 7 143382448 143382592 145 + 1.181 0.832 -1.287
ENSG00000159840 E015 476.671341 0.0007547346 5.484178e-05 3.059097e-04 7 143382593 143382685 93 + 2.658 2.568 -0.299
ENSG00000159840 E016 467.604626 0.0005485990 1.962884e-02 5.069354e-02 7 143382801 143382849 49 + 2.621 2.570 -0.171
ENSG00000159840 E017 835.750354 0.0007115057 3.815056e-02 8.763910e-02 7 143382850 143382999 150 + 2.863 2.826 -0.126
ENSG00000159840 E018 1385.036153 0.0007664464 7.912632e-01 8.665132e-01 7 143383000 143383322 323 + 3.050 3.055 0.017
ENSG00000159840 E019 3.626546 0.0317814410 5.293062e-05 2.964450e-04 7 143384090 143384245 156 + 1.003 0.364 -2.791
ENSG00000159840 E020 1.255549 0.0205331388 3.767680e-03 1.248973e-02 7 143387762 143387787 26 + 0.643 0.138 -3.182
ENSG00000159840 E021 7.009298 0.0024418282 1.253410e-01 2.276303e-01 7 143387889 143388218 330 + 0.977 0.782 -0.750
ENSG00000159840 E022 789.991620 0.0001443997 1.611919e-03 5.999004e-03 7 143388219 143388339 121 + 2.771 2.819 0.158
ENSG00000159840 E023 853.412770 0.0001634896 3.437752e-08 3.826980e-07 7 143388489 143388658 170 + 2.776 2.859 0.278
ENSG00000159840 E024 1101.208899 0.0017796532 2.865639e-03 9.869591e-03 7 143388767 143388945 179 + 2.900 2.970 0.231
ENSG00000159840 E025 1126.972816 0.0021184250 7.789418e-03 2.325043e-02 7 143389857 143389977 121 + 2.914 2.980 0.218
ENSG00000159840 E026 1800.561352 0.0038784655 7.186433e-06 4.934571e-05 7 143390578 143391111 534 + 3.067 3.194 0.424