ENSG00000159423

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000375341 ENSG00000159423 HEK293_OSMI2_2hA HEK293_TMG_2hB ALDH4A1 protein_coding protein_coding 19.70194 36.81802 10.36175 2.047412 0.2847123 -1.828145 14.306017 25.097464 8.137737 1.120924 0.1679613 -1.623645 0.7392583 0.6825667 0.7863 0.1037333 0.03400118 0.03400118 FALSE TRUE
ENST00000454547 ENSG00000159423 HEK293_OSMI2_2hA HEK293_TMG_2hB ALDH4A1 protein_coding processed_transcript 19.70194 36.81802 10.36175 2.047412 0.2847123 -1.828145 2.756591 7.002294 1.371170 1.043309 0.1843834 -2.343996 0.1307250 0.1893000 0.1317 -0.0576000 0.29796672 0.03400118 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000159423 E001 0.1482932 0.0415114772 1.000000e+00   1 18871430 18871431 2 - 0.001 0.068 6.644
ENSG00000159423 E002 235.7733452 0.0002136371 7.276436e-01 8.202725e-01 1 18871432 18871790 359 - 2.263 2.263 0.000
ENSG00000159423 E003 341.9367679 0.0031373313 1.213152e-01 2.218009e-01 1 18871791 18872006 216 - 2.372 2.438 0.222
ENSG00000159423 E004 623.9140668 0.0029899893 1.825966e-02 4.769785e-02 1 18872007 18872732 726 - 2.620 2.700 0.267
ENSG00000159423 E005 133.6425536 0.0011352769 8.788978e-01 9.261933e-01 1 18872733 18872803 71 - 2.011 2.016 0.015
ENSG00000159423 E006 181.7169942 0.0005657061 3.072131e-01 4.494112e-01 1 18872804 18872957 154 - 2.166 2.143 -0.079
ENSG00000159423 E007 155.3786425 0.0002081859 2.581144e-01 3.953223e-01 1 18874463 18874581 119 - 2.105 2.076 -0.098
ENSG00000159423 E008 149.3018810 0.0002324963 3.474807e-01 4.917090e-01 1 18875382 18875503 122 - 2.084 2.060 -0.078
ENSG00000159423 E009 138.9337206 0.0018050789 4.750977e-01 6.138589e-01 1 18876315 18876467 153 - 2.051 2.033 -0.062
ENSG00000159423 E010 87.3333168 0.0003979559 1.267722e-01 2.296569e-01 1 18877208 18877255 48 - 1.881 1.821 -0.203
ENSG00000159423 E011 138.5440979 0.0004354778 2.908634e-02 7.017101e-02 1 18877416 18877612 197 - 2.086 2.016 -0.235
ENSG00000159423 E012 99.8654736 0.0003144058 1.736239e-02 4.573982e-02 1 18879300 18879373 74 - 1.964 1.874 -0.303
ENSG00000159423 E013 136.3243878 0.0003853098 1.127211e-05 7.400425e-05 1 18881700 18881887 188 - 2.138 1.991 -0.491
ENSG00000159423 E014 2.2745721 0.0112077597 5.237471e-01 6.573800e-01 1 18882560 18882561 2 - 0.541 0.432 -0.539
ENSG00000159423 E015 29.4705573 0.0080565250 1.280414e-02 3.545524e-02 1 18882562 18882672 111 - 1.165 1.421 0.894
ENSG00000159423 E016 12.6802334 0.0048551436 5.635718e-02 1.203483e-01 1 18882673 18882675 3 - 0.818 1.076 0.970
ENSG00000159423 E017 20.9459075 0.0014929846 1.074826e-04 5.565435e-04 1 18883111 18883123 13 - 0.818 1.282 1.701
ENSG00000159423 E018 150.0136667 0.0014726217 8.317016e-01 8.945348e-01 1 18883124 18883198 75 - 2.049 2.069 0.066
ENSG00000159423 E019 215.4787291 0.0018177185 6.194787e-01 7.368665e-01 1 18883279 18883428 150 - 2.198 2.227 0.098
ENSG00000159423 E020 200.5881598 0.0008177767 3.223673e-01 4.656722e-01 1 18885473 18885628 156 - 2.208 2.187 -0.072
ENSG00000159423 E021 118.9217593 0.0003009656 2.637832e-01 4.017021e-01 1 18886464 18886511 48 - 1.918 1.974 0.188
ENSG00000159423 E022 157.3009437 0.0043213602 4.199012e-01 5.631618e-01 1 18889362 18889454 93 - 2.044 2.096 0.173
ENSG00000159423 E023 130.7665443 0.0053873448 5.103141e-01 6.455450e-01 1 18890012 18890105 94 - 2.024 2.005 -0.066
ENSG00000159423 E024 0.4772466 0.0220444176 6.100913e-01 7.294597e-01 1 18890694 18890891 198 - 0.209 0.128 -0.860
ENSG00000159423 E025 104.2605440 0.0088505619 6.785302e-01 7.829834e-01 1 18902462 18902731 270 - 1.918 1.909 -0.032