ENSG00000159335

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000309083 ENSG00000159335 HEK293_OSMI2_2hA HEK293_TMG_2hB PTMS protein_coding protein_coding 198.3768 430.0657 95.60581 8.935625 1.399992 -2.169269 131.52800 222.0739 87.185287 23.13136 1.944205 -1.348783 0.7034000 0.5164333 0.91196667 0.3955333 4.149062e-09 4.149062e-09 FALSE FALSE
ENST00000540667 ENSG00000159335 HEK293_OSMI2_2hA HEK293_TMG_2hB PTMS protein_coding processed_transcript 198.3768 430.0657 95.60581 8.935625 1.399992 -2.169269 58.13745 190.9257 2.649389 24.30964 1.327632 -6.165849 0.2496417 0.4439000 0.02746667 -0.4164333 6.022144e-02 4.149062e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000159335 E001 0.000000       12 6765516 6765638 123 +      
ENSG00000159335 E002 31.546424 0.0158684866 1.726332e-03 6.365907e-03 12 6766363 6766372 10 + 1.597 1.313 -0.980
ENSG00000159335 E003 35.090563 0.0287558358 2.486566e-03 8.723994e-03 12 6766373 6766374 2 + 1.661 1.352 -1.060
ENSG00000159335 E004 73.473249 0.0472548912 4.785926e-03 1.533444e-02 12 6766375 6766378 4 + 1.977 1.667 -1.046
ENSG00000159335 E005 97.108181 0.0479157087 6.285181e-03 1.936049e-02 12 6766379 6766397 19 + 2.085 1.791 -0.990
ENSG00000159335 E006 124.535092 0.0467945859 1.069268e-02 3.043642e-02 12 6766398 6766430 33 + 2.170 1.906 -0.886
ENSG00000159335 E007 1271.418042 0.0049683715 2.051065e-08 2.386195e-07 12 6766431 6766750 320 + 3.118 2.930 -0.626
ENSG00000159335 E008 4.626176 0.0037736064 3.501760e-05 2.047272e-04 12 6769053 6769177 125 + 1.054 0.477 -2.367
ENSG00000159335 E009 1914.730727 0.0002535194 2.198291e-09 3.056809e-08 12 6769603 6769674 72 + 3.199 3.129 -0.234
ENSG00000159335 E010 78.782298 0.0026692502 5.622148e-03 1.761035e-02 12 6769844 6769920 77 + 1.881 1.732 -0.503
ENSG00000159335 E011 1264.679789 0.0001094817 1.872367e-04 9.090578e-04 12 6769921 6769923 3 + 3.002 2.953 -0.161
ENSG00000159335 E012 2985.354691 0.0006453678 4.523348e-02 1.006205e-01 12 6769924 6769999 76 + 3.309 3.340 0.102
ENSG00000159335 E013 1969.685368 0.0003888511 3.431363e-01 4.871965e-01 12 6770157 6770168 12 + 3.142 3.157 0.049
ENSG00000159335 E014 1806.694044 0.0006131598 1.127894e-01 2.096117e-01 12 6770169 6770171 3 + 3.093 3.120 0.091
ENSG00000159335 E015 2651.909793 0.0001866565 2.894213e-07 2.687745e-06 12 6770172 6770218 47 + 3.235 3.292 0.188
ENSG00000159335 E016 5107.146517 0.0013537611 9.776904e-07 8.127271e-06 12 6770392 6770952 561 + 3.502 3.580 0.259