ENSG00000159263

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000290399 ENSG00000159263 HEK293_OSMI2_2hA HEK293_TMG_2hB SIM2 protein_coding protein_coding 6.16534 8.003547 5.128017 0.5866734 0.06738072 -0.6412293 2.8974031 3.3374589 2.9520950 0.26533028 0.09941294 -0.1764484 0.47921667 0.41690000 0.57633333 1.594333e-01 1.018209e-02 2.584919e-07 FALSE TRUE
ENST00000431229 ENSG00000159263 HEK293_OSMI2_2hA HEK293_TMG_2hB SIM2 protein_coding protein_coding 6.16534 8.003547 5.128017 0.5866734 0.06738072 -0.6412293 0.3439335 0.1699791 0.5714370 0.16997912 0.10107036 1.6917934 0.05649167 0.02150000 0.11093333 8.943333e-02 1.197242e-01 2.584919e-07 FALSE TRUE
ENST00000460783 ENSG00000159263 HEK293_OSMI2_2hA HEK293_TMG_2hB SIM2 protein_coding processed_transcript 6.16534 8.003547 5.128017 0.5866734 0.06738072 -0.6412293 0.5748293 0.7708497 0.2957488 0.03632821 0.05248281 -1.3526982 0.09345833 0.09670000 0.05743333 -3.926667e-02 1.918648e-01 2.584919e-07 FALSE TRUE
ENST00000481185 ENSG00000159263 HEK293_OSMI2_2hA HEK293_TMG_2hB SIM2 protein_coding retained_intron 6.16534 8.003547 5.128017 0.5866734 0.06738072 -0.6412293 0.3054167 0.2719702 0.3330168 0.10109292 0.11897715 0.2827365 0.05386667 0.03303333 0.06463333 3.160000e-02 5.459186e-01 2.584919e-07 FALSE TRUE
ENST00000483178 ENSG00000159263 HEK293_OSMI2_2hA HEK293_TMG_2hB SIM2 protein_coding protein_coding 6.16534 8.003547 5.128017 0.5866734 0.06738072 -0.6412293 1.6031052 2.8631597 0.5949665 0.30595733 0.07217149 -2.2477111 0.24543333 0.35733333 0.11620000 -2.411333e-01 2.584919e-07 2.584919e-07   FALSE
MSTRG.21318.4 ENSG00000159263 HEK293_OSMI2_2hA HEK293_TMG_2hB SIM2 protein_coding   6.16534 8.003547 5.128017 0.5866734 0.06738072 -0.6412293 0.4406524 0.5901295 0.3807531 0.04651061 0.04738873 -0.6190164 0.07153333 0.07450000 0.07446667 -3.333333e-05 1.000000e+00 2.584919e-07 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000159263 E001 3.278649 0.0632458195 2.514006e-01 3.876439e-01 21 36699115 36699132 18 + 0.713 0.517 -0.867
ENSG00000159263 E002 3.278649 0.0632458195 2.514006e-01 3.876439e-01 21 36699133 36699133 1 + 0.713 0.517 -0.867
ENSG00000159263 E003 24.165348 0.0408611032 2.831536e-02 6.863529e-02 21 36699134 36699420 287 + 1.521 1.272 -0.859
ENSG00000159263 E004 62.447163 0.0121931693 2.203090e-01 3.507949e-01 21 36699421 36699921 501 + 1.835 1.756 -0.268
ENSG00000159263 E005 23.832406 0.0046430506 7.184221e-01 8.135261e-01 21 36709168 36709180 13 + 1.361 1.389 0.097
ENSG00000159263 E006 36.634214 0.0174505513 6.917826e-01 7.931790e-01 21 36709181 36709250 70 + 1.541 1.571 0.103
ENSG00000159263 E007 25.442998 0.0007944698 9.161475e-10 1.368133e-08 21 36709251 36710613 1363 + 1.050 1.535 1.701
ENSG00000159263 E008 28.004025 0.0029347999 6.918218e-01 7.932025e-01 21 36712533 36712565 33 + 1.463 1.436 -0.093
ENSG00000159263 E009 34.889206 0.0005965871 8.537277e-01 9.094936e-01 21 36712566 36712622 57 + 1.545 1.535 -0.036
ENSG00000159263 E010 47.837081 0.0004529910 8.433181e-01 9.024282e-01 21 36719821 36719929 109 + 1.678 1.668 -0.033
ENSG00000159263 E011 33.103999 0.0041211872 9.826213e-13 2.415397e-11 21 36719930 36720587 658 + 1.092 1.653 1.953
ENSG00000159263 E012 29.863409 0.0007182292 1.786616e-04 8.721406e-04 21 36723045 36723130 86 + 1.607 1.374 -0.800
ENSG00000159263 E013 47.950689 0.0004912868 1.849612e-04 8.990468e-04 21 36726119 36726318 200 + 1.782 1.597 -0.626
ENSG00000159263 E014 39.061185 0.0005928320 7.903388e-02 1.579685e-01 21 36731045 36731151 107 + 1.645 1.549 -0.330
ENSG00000159263 E015 37.345837 0.0005729730 3.486096e-01 4.928476e-01 21 36741717 36741864 148 + 1.598 1.545 -0.181
ENSG00000159263 E016 47.982922 0.0042760179 7.668612e-01 8.492355e-01 21 36743387 36743555 169 + 1.681 1.665 -0.055
ENSG00000159263 E017 88.567560 0.0003893981 9.533623e-01 9.748353e-01 21 36744728 36745136 409 + 1.932 1.935 0.009
ENSG00000159263 E018 7.156260 0.0879430259 5.319153e-01 6.643094e-01 21 36745137 36745794 658 + 0.954 0.846 -0.410
ENSG00000159263 E019 6.053840 0.0028245611 4.029817e-02 9.158530e-02 21 36745795 36746324 530 + 0.970 0.716 -0.991
ENSG00000159263 E020 314.460021 0.0031141431 2.856199e-01 4.259812e-01 21 36747665 36750640 2976 + 2.465 2.496 0.101
ENSG00000159263 E021 49.700160 0.0025009975 1.543277e-02 4.147493e-02 21 36750641 36754420 3780 + 1.602 1.738 0.459