ENSG00000159069

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000325285 ENSG00000159069 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXW5 protein_coding protein_coding 61.36296 104.8059 34.26329 7.457611 1.026055 -1.6127 18.073726 36.536490 7.623078 2.1008835 0.7854331 -2.2593961 0.26888750 0.34980000 0.2240667 -0.12573333 0.028146195 0.001870511 FALSE TRUE
ENST00000480818 ENSG00000159069 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXW5 protein_coding retained_intron 61.36296 104.8059 34.26329 7.457611 1.026055 -1.6127 8.695497 10.478094 6.831476 1.8345422 0.7787596 -0.6163730 0.15873333 0.09846667 0.1987667 0.10030000 0.001870511 0.001870511 TRUE TRUE
ENST00000483559 ENSG00000159069 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXW5 protein_coding retained_intron 61.36296 104.8059 34.26329 7.457611 1.026055 -1.6127 3.817354 5.305854 3.127963 0.9142475 0.6290107 -0.7604731 0.06727917 0.04986667 0.0906000 0.04073333 0.092172152 0.001870511 FALSE TRUE
ENST00000487794 ENSG00000159069 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXW5 protein_coding nonsense_mediated_decay 61.36296 104.8059 34.26329 7.457611 1.026055 -1.6127 27.752050 47.688632 14.972760 3.5915746 1.0647111 -1.6706445 0.45439583 0.45550000 0.4359667 -0.01953333 0.833589168 0.001870511 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000159069 E001 1.9605709 7.387683e-03 6.873207e-03 2.089491e-02 9 136940351 136940434 84 - 0.726 0.269 -2.330
ENSG00000159069 E002 201.3634623 8.469037e-04 2.426435e-11 4.749260e-10 9 136940435 136940529 95 - 1.982 2.234 0.845
ENSG00000159069 E003 570.6524205 1.090004e-03 2.609678e-12 5.988320e-11 9 136940530 136940677 148 - 2.497 2.676 0.599
ENSG00000159069 E004 1653.7865116 7.973871e-04 1.325351e-07 1.315332e-06 9 136940678 136941111 434 - 3.037 3.125 0.293
ENSG00000159069 E005 585.5462911 1.358784e-04 8.088556e-04 3.290005e-03 9 136941112 136941171 60 - 2.604 2.668 0.213
ENSG00000159069 E006 926.0943831 3.048247e-04 4.396457e-02 9.831505e-02 9 136941251 136941410 160 - 2.827 2.861 0.114
ENSG00000159069 E007 536.1883709 2.133409e-04 9.294196e-01 9.595847e-01 9 136941411 136941463 53 - 2.615 2.617 0.008
ENSG00000159069 E008 859.0269401 1.914736e-04 3.933894e-01 5.372746e-01 9 136941537 136941684 148 - 2.832 2.820 -0.041
ENSG00000159069 E009 186.0845226 3.246660e-03 1.113729e-09 1.636997e-08 9 136941755 136942045 291 - 2.345 2.106 -0.799
ENSG00000159069 E010 490.0963952 1.319734e-04 4.272735e-02 9.604718e-02 9 136942046 136942099 54 - 2.611 2.571 -0.132
ENSG00000159069 E011 1161.8658707 8.167557e-05 9.573954e-01 9.772923e-01 9 136942100 136942466 367 - 2.952 2.953 0.004
ENSG00000159069 E012 40.9009638 4.822165e-03 9.077329e-04 3.640983e-03 9 136942467 136942546 80 - 1.686 1.457 -0.780
ENSG00000159069 E013 546.4578759 1.640968e-04 3.949491e-02 9.009847e-02 9 136942547 136942650 104 - 2.657 2.619 -0.129
ENSG00000159069 E014 404.1553544 7.338439e-04 7.326066e-02 1.487329e-01 9 136942651 136942695 45 - 2.530 2.488 -0.142
ENSG00000159069 E015 67.7950136 4.015231e-04 3.901730e-04 1.736693e-03 9 136942696 136942768 73 - 1.861 1.685 -0.593
ENSG00000159069 E016 601.8479907 8.256884e-04 1.551213e-02 4.165570e-02 9 136942769 136942905 137 - 2.710 2.660 -0.167
ENSG00000159069 E017 252.7684454 2.000938e-04 3.230738e-02 7.644745e-02 9 136942906 136942906 1 - 2.340 2.282 -0.195
ENSG00000159069 E018 333.6460147 1.502729e-04 8.933420e-03 2.611219e-02 9 136942907 136942943 37 - 2.462 2.400 -0.206
ENSG00000159069 E019 28.1541290 7.031029e-04 3.713847e-03 1.233675e-02 9 136942944 136942973 30 - 1.519 1.306 -0.737
ENSG00000159069 E020 24.1707231 8.748061e-04 7.997576e-03 2.377325e-02 9 136942974 136943003 30 - 1.449 1.239 -0.733
ENSG00000159069 E021 525.8867330 2.415855e-03 3.737289e-02 8.617343e-02 9 136943349 136943506 158 - 2.659 2.600 -0.195
ENSG00000159069 E022 396.6888123 6.867223e-03 2.059459e-01 3.336085e-01 9 136943891 136944106 216 - 2.532 2.480 -0.172
ENSG00000159069 E023 72.1191525 1.398967e-02 2.001467e-08 2.333366e-07 9 136944107 136944534 428 - 2.040 1.645 -1.331
ENSG00000159069 E024 97.3349531 2.045553e-02 9.175773e-01 9.519186e-01 9 136944594 136945485 892 - 1.867 1.887 0.066
ENSG00000159069 E025 0.9158852 1.464230e-02 1.338122e-01 2.394544e-01 9 136949762 136949971 210 - 0.455 0.180 -1.847