ENSG00000158966

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000290039 ENSG00000158966 HEK293_OSMI2_2hA HEK293_TMG_2hB CACHD1 protein_coding protein_coding 12.52368 7.486875 13.25446 0.447912 0.06056606 0.8232042 9.711018 6.226688 8.679347 0.2976459 0.2461229 0.4784675 0.7769042 0.8346667 0.6550333 -0.1796333 0.01934695 0.01934695 FALSE TRUE
ENST00000651257 ENSG00000158966 HEK293_OSMI2_2hA HEK293_TMG_2hB CACHD1 protein_coding protein_coding 12.52368 7.486875 13.25446 0.447912 0.06056606 0.8232042 2.677100 1.245221 4.475277 0.3135136 0.2932449 1.8372554 0.2137042 0.1633667 0.3374333 0.1740667 0.02071743 0.01934695 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000158966 E001 47.1306358 0.0059303217 1.141733e-04 5.870001e-04 1 64470129 64470792 664 + 1.727 1.530 -0.670
ENSG00000158966 E002 32.6020415 0.0006014757 4.033857e-04 1.787565e-03 1 64470793 64470942 150 + 1.568 1.395 -0.595
ENSG00000158966 E003 0.0000000       1 64505901 64505914 14 +      
ENSG00000158966 E004 0.0000000       1 64505915 64506487 573 +      
ENSG00000158966 E005 36.3600211 0.0006043926 1.239188e-05 8.057681e-05 1 64550594 64550656 63 + 1.628 1.411 -0.743
ENSG00000158966 E006 0.0000000       1 64563677 64563844 168 +      
ENSG00000158966 E007 58.1674444 0.0041333092 9.849551e-07 8.182634e-06 1 64582156 64582304 149 + 1.830 1.600 -0.779
ENSG00000158966 E008 56.4898564 0.0072052913 1.199359e-05 7.824743e-05 1 64602806 64602912 107 + 1.813 1.586 -0.770
ENSG00000158966 E009 0.1451727 0.0430958054 8.435681e-01   1 64613488 64613518 31 + 0.089 0.000 -8.406
ENSG00000158966 E010 65.0829239 0.0085401059 9.058237e-05 4.777438e-04 1 64629355 64629481 127 + 1.866 1.672 -0.655
ENSG00000158966 E011 70.4955284 0.0005061643 2.726203e-07 2.543816e-06 1 64632599 64632743 145 + 1.892 1.716 -0.595
ENSG00000158966 E012 95.2596140 0.0030751141 9.784242e-04 3.886107e-03 1 64634044 64634260 217 + 1.994 1.904 -0.303
ENSG00000158966 E013 83.3171792 0.0064622130 2.214181e-01 3.521229e-01 1 64641820 64641969 150 + 1.908 1.903 -0.016
ENSG00000158966 E014 88.9658463 0.0028191132 7.772734e-05 4.173340e-04 1 64647801 64648034 234 + 1.976 1.852 -0.418
ENSG00000158966 E015 67.2632634 0.0004468600 1.850864e-04 8.995609e-04 1 64652161 64652310 150 + 1.851 1.739 -0.379
ENSG00000158966 E016 62.6582135 0.0006485287 7.005401e-03 2.123749e-02 1 64653758 64653881 124 + 1.811 1.742 -0.230
ENSG00000158966 E017 63.6307453 0.0006383124 2.084806e-03 7.486690e-03 1 64654686 64654803 118 + 1.824 1.739 -0.289
ENSG00000158966 E018 70.2987921 0.0087650637 6.354551e-03 1.954366e-02 1 64658705 64658873 169 + 1.874 1.762 -0.378
ENSG00000158966 E019 75.9617128 0.0032154319 1.561779e-01 2.700421e-01 1 64663695 64663837 143 + 1.872 1.862 -0.033
ENSG00000158966 E020 0.0000000       1 64664200 64664497 298 +      
ENSG00000158966 E021 93.9039917 0.0005550365 2.007088e-01 3.270825e-01 1 64664498 64664679 182 + 1.952 1.963 0.036
ENSG00000158966 E022 74.0299442 0.0003621375 1.089786e-01 2.040267e-01 1 64666057 64666167 111 + 1.858 1.849 -0.029
ENSG00000158966 E023 75.7006817 0.0004052742 1.589376e-01 2.737139e-01 1 64671564 64671686 123 + 1.863 1.864 0.001
ENSG00000158966 E024 64.5586575 0.0092261322 8.899452e-01 9.336648e-01 1 64673158 64673257 100 + 1.775 1.842 0.224
ENSG00000158966 E025 68.1559841 0.0009812345 6.794470e-01 7.836271e-01 1 64673348 64673464 117 + 1.806 1.847 0.138
ENSG00000158966 E026 81.8937861 0.0003587413 5.326024e-01 6.649351e-01 1 64675401 64675561 161 + 1.888 1.923 0.117
ENSG00000158966 E027 69.7087766 0.0003571486 9.436786e-01 9.686717e-01 1 64675897 64675983 87 + 1.809 1.872 0.211
ENSG00000158966 E028 65.8524857 0.0004051315 1.356932e-01 2.420818e-01 1 64676895 64677011 117 + 1.757 1.882 0.423
ENSG00000158966 E029 77.1293384 0.0003287067 2.313417e-01 3.640841e-01 1 64678159 64678310 152 + 1.831 1.940 0.365
ENSG00000158966 E030 92.6827131 0.0020043539 2.948812e-01 4.359872e-01 1 64679595 64679756 162 + 1.916 2.020 0.351
ENSG00000158966 E031 79.7612494 0.0059050998 8.512195e-01 9.077506e-01 1 64681258 64681335 78 + 1.865 1.936 0.239
ENSG00000158966 E032 83.3067304 0.0004982941 4.416443e-01 5.832852e-01 1 64682005 64682106 102 + 1.874 1.965 0.304
ENSG00000158966 E033 614.2185072 0.0059999357 2.619828e-17 1.210050e-15 1 64691323 64693058 1736 + 2.620 2.952 1.104