ENSG00000158828

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000321556 ENSG00000158828 HEK293_OSMI2_2hA HEK293_TMG_2hB PINK1 protein_coding protein_coding 59.87743 110.3831 33.76597 1.367814 0.6977916 -1.70858 45.32003 76.96525 30.176587 4.601720 1.442185 -1.350487 0.7665917 0.6964667 0.89316667 0.1967000 0.02407994 0.02407994 FALSE TRUE
ENST00000400490 ENSG00000158828 HEK293_OSMI2_2hA HEK293_TMG_2hB PINK1 protein_coding processed_transcript 59.87743 110.3831 33.76597 1.367814 0.6977916 -1.70858 14.06917 33.06579 3.270064 3.316958 1.065330 -3.333980 0.2214333 0.3003333 0.09736667 -0.2029667 0.02614500 0.02407994 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000158828 E001 74.9031127 0.0424434346 4.472163e-08 4.870023e-07 1 20633458 20633678 221 + 2.168 1.590 -1.951
ENSG00000158828 E002 370.4974900 0.0050339571 1.678685e-06 1.327636e-05 1 20633679 20633935 257 + 2.610 2.423 -0.624
ENSG00000158828 E003 821.6452882 0.0014694313 1.074500e-01 2.017791e-01 1 20637842 20638128 287 + 2.842 2.804 -0.124
ENSG00000158828 E004 289.9795584 0.0011212702 9.231794e-01 9.555563e-01 1 20638129 20638129 1 + 2.363 2.359 -0.015
ENSG00000158828 E005 6.5539217 0.0026813993 1.059877e-01 1.995949e-01 1 20638130 20639891 1762 + 0.948 0.731 -0.844
ENSG00000158828 E006 563.5264643 0.0001895039 5.368453e-01 6.685370e-01 1 20639892 20639992 101 + 2.659 2.646 -0.044
ENSG00000158828 E007 720.1913146 0.0001316038 9.984322e-01 1.000000e+00 1 20644490 20644672 183 + 2.755 2.753 -0.006
ENSG00000158828 E008 0.4031496 0.0251045829 8.517106e-01 9.080522e-01 1 20645508 20645559 52 + 0.000 0.131 8.626
ENSG00000158828 E009 742.3030314 0.0006776120 5.713104e-01 6.976206e-01 1 20645560 20645723 164 + 2.759 2.769 0.032
ENSG00000158828 E010 4.5629598 0.0037373422 6.158433e-01 7.340070e-01 1 20646567 20648504 1938 + 0.605 0.686 0.348
ENSG00000158828 E011 754.5315572 0.0001770426 1.908836e-01 3.148465e-01 1 20648505 20648632 128 + 2.757 2.777 0.065
ENSG00000158828 E012 15.0734846 0.0012301654 4.663135e-03 1.499350e-02 1 20648633 20648994 362 + 1.309 1.041 -0.955
ENSG00000158828 E013 1177.1266069 0.0002805747 2.154315e-02 5.473167e-02 1 20648995 20649231 237 + 2.943 2.974 0.102
ENSG00000158828 E014 427.4445371 0.0010101325 6.716975e-01 7.778754e-01 1 20650434 20650435 2 + 2.522 2.532 0.034
ENSG00000158828 E015 2345.0595992 0.0020472099 6.114919e-03 1.890920e-02 1 20650436 20651511 1076 + 3.227 3.277 0.168