ENSG00000158773

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000368021 ENSG00000158773 HEK293_OSMI2_2hA HEK293_TMG_2hB USF1 protein_coding protein_coding 65.16117 98.97112 41.95699 2.420679 1.09958 -1.237898 37.446342 67.763168 19.843291 1.2934964 0.3494397 -1.7713360 0.54396667 0.68483333 0.47380000 -0.21103333 4.437987e-13 4.437987e-13 FALSE TRUE
ENST00000472217 ENSG00000158773 HEK293_OSMI2_2hA HEK293_TMG_2hB USF1 protein_coding retained_intron 65.16117 98.97112 41.95699 2.420679 1.09958 -1.237898 12.158474 13.944446 8.187951 0.9918324 0.9795948 -0.7673896 0.19652083 0.14073333 0.19433333 0.05360000 1.392402e-01 4.437987e-13 TRUE FALSE
ENST00000473969 ENSG00000158773 HEK293_OSMI2_2hA HEK293_TMG_2hB USF1 protein_coding nonsense_mediated_decay 65.16117 98.97112 41.95699 2.420679 1.09958 -1.237898 10.837751 13.449795 8.146879 0.8024216 1.0913955 -0.7225673 0.17409167 0.13586667 0.19323333 0.05736667 1.835179e-01 4.437987e-13 FALSE TRUE
ENST00000496363 ENSG00000158773 HEK293_OSMI2_2hA HEK293_TMG_2hB USF1 protein_coding retained_intron 65.16117 98.97112 41.95699 2.420679 1.09958 -1.237898 2.601692 1.200114 3.039713 0.0376981 0.3784615 1.3335301 0.04896667 0.01216667 0.07293333 0.06076667 4.624143e-12 4.437987e-13 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000158773 E001 34.424042 0.0071146926 9.961570e-07 8.267357e-06 1 161039251 161039294 44 - 1.064 1.545 1.685
ENSG00000158773 E002 719.142774 0.0034066850 6.481759e-05 3.549911e-04 1 161039295 161039635 341 - 2.668 2.803 0.451
ENSG00000158773 E003 782.175016 0.0006532468 1.929163e-11 3.833780e-10 1 161039636 161039854 219 - 2.703 2.841 0.460
ENSG00000158773 E004 505.365487 0.0011694198 1.202466e-05 7.842315e-05 1 161039855 161039919 65 - 2.530 2.648 0.395
ENSG00000158773 E005 357.465218 0.0004039995 1.004365e-04 5.238964e-04 1 161039920 161039939 20 - 2.392 2.497 0.348
ENSG00000158773 E006 466.207185 0.0002668426 4.918307e-05 2.776148e-04 1 161039940 161040009 70 - 2.514 2.609 0.314
ENSG00000158773 E007 281.287095 0.0001732750 2.407950e-01 3.751124e-01 1 161040202 161040208 7 - 2.340 2.379 0.131
ENSG00000158773 E008 423.252126 0.0006481937 1.207149e-01 2.209850e-01 1 161040209 161040288 80 - 2.511 2.557 0.151
ENSG00000158773 E009 261.583181 0.0006066340 4.280546e-01 5.708650e-01 1 161040289 161040323 35 - 2.314 2.345 0.104
ENSG00000158773 E010 189.323045 0.0002002473 3.759617e-01 5.203411e-01 1 161040324 161040330 7 - 2.172 2.209 0.123
ENSG00000158773 E011 67.997100 0.0012741505 2.469271e-15 8.874899e-14 1 161040331 161040439 109 - 2.010 1.649 -1.220
ENSG00000158773 E012 64.752922 0.0016124869 9.317785e-14 2.661680e-12 1 161040440 161040575 136 - 1.986 1.633 -1.191
ENSG00000158773 E013 394.318982 0.0003259367 1.256413e-01 2.280640e-01 1 161040576 161040670 95 - 2.482 2.525 0.144
ENSG00000158773 E014 350.727040 0.0002307672 7.803561e-02 1.563196e-01 1 161040814 161040872 59 - 2.489 2.459 -0.102
ENSG00000158773 E015 233.974577 0.0003835373 5.884183e-02 1.246454e-01 1 161041324 161041325 2 - 2.325 2.283 -0.143
ENSG00000158773 E016 311.299565 0.0002587902 9.971290e-01 1.000000e+00 1 161041326 161041389 64 - 2.407 2.417 0.031
ENSG00000158773 E017 203.143148 0.0001909970 6.403413e-01 7.535956e-01 1 161041390 161041411 22 - 2.233 2.228 -0.014
ENSG00000158773 E018 5.938858 0.0028410363 1.276036e-05 8.271442e-05 1 161041412 161041606 195 - 1.122 0.586 -2.103
ENSG00000158773 E019 3.892472 0.0550634848 2.104559e-01 3.390730e-01 1 161041607 161041650 44 - 0.779 0.566 -0.904
ENSG00000158773 E020 288.658408 0.0002156892 1.386096e-01 2.461744e-01 1 161041651 161041734 84 - 2.402 2.375 -0.091
ENSG00000158773 E021 380.835052 0.0001946995 4.902868e-07 4.342373e-06 1 161041735 161041833 99 - 2.574 2.478 -0.320
ENSG00000158773 E022 260.901360 0.0002805845 9.927810e-08 1.008469e-06 1 161041834 161041846 13 - 2.431 2.305 -0.418
ENSG00000158773 E023 435.716697 0.0002534702 9.155464e-12 1.920154e-10 1 161042116 161042217 102 - 2.654 2.529 -0.419
ENSG00000158773 E024 220.871348 0.0018312202 5.842311e-06 4.096104e-05 1 161042555 161042585 31 - 2.372 2.230 -0.473
ENSG00000158773 E025 434.772790 0.0012767928 1.833817e-03 6.707606e-03 1 161042586 161042670 85 - 2.613 2.542 -0.236
ENSG00000158773 E026 397.101840 0.0004025721 1.999377e-02 5.147462e-02 1 161042833 161042882 50 - 2.552 2.509 -0.142
ENSG00000158773 E027 9.576515 0.0043332549 7.719874e-01 8.528519e-01 1 161043253 161043267 15 - 0.971 0.944 -0.100
ENSG00000158773 E028 435.329699 0.0005942012 2.109554e-01 3.396640e-01 1 161043268 161043360 93 - 2.574 2.555 -0.065
ENSG00000158773 E029 1.033047 0.0120236175 2.409826e-05 1.467437e-04 1 161044912 161044941 30 - 0.690 0.000 -13.499
ENSG00000158773 E030 310.095412 0.0009883355 9.791933e-01 9.910506e-01 1 161045858 161045977 120 - 2.407 2.414 0.024