ENSG00000158636

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000525919 ENSG00000158636 HEK293_OSMI2_2hA HEK293_TMG_2hB EMSY protein_coding protein_coding 6.862643 3.7826 9.367527 0.1775146 0.1405411 1.30602 0.0916509 0.29672987 0.00000000 0.29672987 0.00000000 -4.9388967 0.02297917 0.07810000 0.000000000 -0.07810000 6.301351e-01 3.153746e-17 FALSE TRUE
ENST00000531793 ENSG00000158636 HEK293_OSMI2_2hA HEK293_TMG_2hB EMSY protein_coding protein_coding 6.862643 3.7826 9.367527 0.1775146 0.1405411 1.30602 0.1500740 0.36759295 0.05684497 0.18701354 0.01677797 -2.4979408 0.03677917 0.09303333 0.006033333 -0.08700000 1.362728e-01 3.153746e-17 FALSE TRUE
ENST00000531998 ENSG00000158636 HEK293_OSMI2_2hA HEK293_TMG_2hB EMSY protein_coding protein_coding 6.862643 3.7826 9.367527 0.1775146 0.1405411 1.30602 0.2180017 0.32431952 0.21926683 0.09939829 0.03284261 -0.5442000 0.03893750 0.08400000 0.023300000 -0.06070000 2.846827e-02 3.153746e-17 FALSE FALSE
ENST00000533988 ENSG00000158636 HEK293_OSMI2_2hA HEK293_TMG_2hB EMSY protein_coding protein_coding 6.862643 3.7826 9.367527 0.1775146 0.1405411 1.30602 1.2329450 0.57152271 2.40436706 0.19756216 0.09876923 2.0537376 0.16109583 0.15280000 0.256700000 0.10390000 3.229075e-01 3.153746e-17   FALSE
ENST00000534573 ENSG00000158636 HEK293_OSMI2_2hA HEK293_TMG_2hB EMSY protein_coding retained_intron 6.862643 3.7826 9.367527 0.1775146 0.1405411 1.30602 0.8700579 0.23091891 1.12076861 0.12149014 0.17822052 2.2306842 0.12455833 0.06423333 0.119133333 0.05490000 5.660261e-01 3.153746e-17 FALSE FALSE
ENST00000695367 ENSG00000158636 HEK293_OSMI2_2hA HEK293_TMG_2hB EMSY protein_coding protein_coding 6.862643 3.7826 9.367527 0.1775146 0.1405411 1.30602 0.4955560 0.23638741 0.78766982 0.23638741 0.16684848 1.6948632 0.08607083 0.05790000 0.083700000 0.02580000 4.328128e-01 3.153746e-17 FALSE TRUE
MSTRG.6025.11 ENSG00000158636 HEK293_OSMI2_2hA HEK293_TMG_2hB EMSY protein_coding   6.862643 3.7826 9.367527 0.1775146 0.1405411 1.30602 1.0345057 0.42297517 1.48022038 0.42297517 0.09857680 1.7831695 0.14145833 0.10363333 0.158166667 0.05453333 3.226015e-01 3.153746e-17 FALSE TRUE
MSTRG.6025.12 ENSG00000158636 HEK293_OSMI2_2hA HEK293_TMG_2hB EMSY protein_coding   6.862643 3.7826 9.367527 0.1775146 0.1405411 1.30602 1.1900332 0.02254611 2.03060008 0.01211914 0.02037536 5.9703645 0.12304583 0.00630000 0.216800000 0.21050000 3.153746e-17 3.153746e-17 FALSE TRUE
MSTRG.6025.13 ENSG00000158636 HEK293_OSMI2_2hA HEK293_TMG_2hB EMSY protein_coding   6.862643 3.7826 9.367527 0.1775146 0.1405411 1.30602 0.1186679 0.33540524 0.00000000 0.16772830 0.00000000 -5.1102181 0.03005417 0.09246667 0.000000000 -0.09246667 5.869441e-02 3.153746e-17 FALSE TRUE
MSTRG.6025.8 ENSG00000158636 HEK293_OSMI2_2hA HEK293_TMG_2hB EMSY protein_coding   6.862643 3.7826 9.367527 0.1775146 0.1405411 1.30602 0.6877594 0.53827885 0.31498954 0.53827885 0.31498954 -0.7545166 0.11845000 0.15523333 0.034633333 -0.12060000 9.280080e-01 3.153746e-17 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000158636 E001 1.0351233 0.0119551681 4.035123e-02 9.166530e-02 11 76444923 76445017 95 + 0.400 0.000 -11.018
ENSG00000158636 E002 0.5975289 0.0292378055 1.666366e-01 2.838104e-01 11 76445018 76445021 4 + 0.270 0.000 -12.653
ENSG00000158636 E003 1.0330470 0.0118541788 4.038164e-02 9.171780e-02 11 76445022 76445024 3 + 0.400 0.000 -13.491
ENSG00000158636 E004 1.1782197 0.0108647759 2.602832e-02 6.400305e-02 11 76445025 76445026 2 + 0.435 0.000 -13.663
ENSG00000158636 E005 3.3851167 0.0050064773 5.007878e-02 1.093017e-01 11 76445027 76445041 15 + 0.707 0.393 -1.476
ENSG00000158636 E006 3.2336013 0.0098005533 6.936516e-02 1.423082e-01 11 76445042 76445043 2 + 0.689 0.393 -1.397
ENSG00000158636 E007 3.5970100 0.0516507530 3.426675e-01 4.866805e-01 11 76445044 76445046 3 + 0.688 0.540 -0.652
ENSG00000158636 E008 20.4077359 0.0242991743 7.987975e-03 2.374806e-02 11 76445047 76445128 82 + 1.388 1.108 -0.989
ENSG00000158636 E009 21.3510697 0.0125415916 3.018926e-03 1.032228e-02 11 76446900 76446938 39 + 1.408 1.138 -0.947
ENSG00000158636 E010 25.2605833 0.0075319083 2.060586e-02 5.276080e-02 11 76446939 76447008 70 + 1.453 1.282 -0.593
ENSG00000158636 E011 34.8209984 0.0020962826 6.219953e-04 2.613448e-03 11 76451858 76451957 100 + 1.595 1.390 -0.704
ENSG00000158636 E012 29.0209646 0.0128874919 4.972956e-02 1.086809e-01 11 76453314 76453388 75 + 1.503 1.362 -0.485
ENSG00000158636 E013 18.7689452 0.0728603718 3.135322e-01 4.561478e-01 11 76453389 76453931 543 + 1.315 1.209 -0.375
ENSG00000158636 E014 3.5784744 0.0043981963 2.836894e-01 4.238394e-01 11 76454749 76454790 42 + 0.689 0.538 -0.662
ENSG00000158636 E015 0.0000000       11 76458161 76458182 22 +      
ENSG00000158636 E016 26.7961120 0.0031640750 3.197849e-02 7.581860e-02 11 76458183 76458358 176 + 1.470 1.334 -0.468
ENSG00000158636 E017 0.0000000       11 76458359 76459932 1574 +      
ENSG00000158636 E018 6.2536607 0.0090876907 3.091197e-01 4.513816e-01 11 76459933 76459935 3 + 0.886 0.771 -0.450
ENSG00000158636 E019 18.2449753 0.0024305894 5.273109e-03 1.667515e-02 11 76459936 76460085 150 + 1.332 1.106 -0.800
ENSG00000158636 E020 0.3332198 0.0290940645 5.809320e-01   11 76460086 76461015 930 + 0.085 0.173 1.186
ENSG00000158636 E021 44.3373767 0.0037393463 9.152847e-06 6.137980e-05 11 76463821 76464080 260 + 1.710 1.447 -0.898
ENSG00000158636 E022 44.3338487 0.0005845134 7.784082e-02 1.560102e-01 11 76472564 76472840 277 + 1.660 1.589 -0.241
ENSG00000158636 E023 39.3828194 0.0005524363 1.286450e-02 3.559898e-02 11 76496215 76496469 255 + 1.625 1.504 -0.414
ENSG00000158636 E024 0.5996172 0.4188136515 5.724790e-01 6.985927e-01 11 76496470 76497231 762 + 0.156 0.289 1.123
ENSG00000158636 E025 12.9930171 0.0020355836 7.293362e-01 8.215306e-01 11 76513386 76513396 11 + 1.131 1.123 -0.028
ENSG00000158636 E026 31.7925146 0.0006879836 7.175557e-02 1.462698e-01 11 76513397 76513535 139 + 1.523 1.432 -0.315
ENSG00000158636 E027 35.0492516 0.0007812008 9.281508e-04 3.713289e-03 11 76516142 76516312 171 + 1.588 1.399 -0.650
ENSG00000158636 E028 4.2822576 0.0237829030 4.797084e-03 1.536655e-02 11 76516313 76516842 530 + 0.499 0.946 1.860
ENSG00000158636 E029 30.4406610 0.0026365383 1.043981e-01 1.971855e-01 11 76523155 76523291 137 + 1.503 1.416 -0.301
ENSG00000158636 E030 17.4477751 0.0014225794 8.212906e-01 8.873354e-01 11 76526462 76526478 17 + 1.241 1.248 0.026
ENSG00000158636 E031 38.4084053 0.0009766717 5.902042e-01 7.131121e-01 11 76526479 76526635 157 + 1.571 1.567 -0.015
ENSG00000158636 E032 40.0397443 0.0133099631 6.766682e-01 7.815822e-01 11 76528268 76528466 199 + 1.586 1.589 0.011
ENSG00000158636 E033 32.4611038 0.0006878631 2.031495e-01 3.301654e-01 11 76535895 76536059 165 + 1.518 1.462 -0.190
ENSG00000158636 E034 34.0614907 0.0006451990 5.516563e-02 1.182798e-01 11 76537795 76537950 156 + 1.550 1.455 -0.328
ENSG00000158636 E035 14.1712529 0.0029194497 8.048178e-02 1.603078e-01 11 76539599 76539640 42 + 1.201 1.054 -0.529
ENSG00000158636 E036 5.1629641 0.0031068532 3.376777e-01 4.815721e-01 11 76542051 76542167 117 + 0.806 0.693 -0.457
ENSG00000158636 E037 4.8587874 0.0032707226 7.311211e-01 8.228550e-01 11 76542168 76542215 48 + 0.759 0.734 -0.103
ENSG00000158636 E038 19.0996793 0.0010919314 5.675442e-01 6.943196e-01 11 76542216 76542238 23 + 1.291 1.271 -0.071
ENSG00000158636 E039 34.4015239 0.0006329105 5.339853e-01 6.660999e-01 11 76542239 76542367 129 + 1.529 1.517 -0.039
ENSG00000158636 E040 76.5888060 0.0011985527 7.621634e-01 8.458377e-01 11 76544259 76544822 564 + 1.849 1.891 0.139
ENSG00000158636 E041 3.5010283 0.0088276834 6.460828e-01 7.580917e-01 11 76544823 76544868 46 + 0.648 0.596 -0.227
ENSG00000158636 E042 34.7243914 0.0016087294 1.840569e-03 6.728226e-03 11 76545797 76545940 144 + 1.436 1.656 0.750
ENSG00000158636 E043 15.7134264 0.0014131249 9.920776e-01 9.991396e-01 11 76545941 76545947 7 + 1.183 1.210 0.097
ENSG00000158636 E044 64.1152957 0.0071301574 2.185769e-03 7.797522e-03 11 76545948 76546297 350 + 1.702 1.911 0.705
ENSG00000158636 E045 0.5911862 0.0184460500 1.644145e-01 2.809286e-01 11 76547028 76547100 73 + 0.270 0.000 -12.728
ENSG00000158636 E046 232.5650444 0.0034082546 8.466876e-25 9.103430e-23 11 76549952 76553031 3080 + 2.192 2.538 1.153