ENSG00000158435

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000289382 ENSG00000158435 HEK293_OSMI2_2hA HEK293_TMG_2hB CNOT11 protein_coding protein_coding 55.01405 51.90196 56.44222 6.167708 0.7902728 0.1209635 29.172846 31.1877708 33.13649 0.6181592 0.4681229 0.08741302 0.53829583 0.614133333 0.5871667 -0.02696667 0.87112959 0.01491765 FALSE TRUE
ENST00000420107 ENSG00000158435 HEK293_OSMI2_2hA HEK293_TMG_2hB CNOT11 protein_coding protein_coding 55.01405 51.90196 56.44222 6.167708 0.7902728 0.1209635 13.568310 17.2846746 8.83526 4.8054720 1.6231074 -0.96735145 0.24780000 0.321300000 0.1566333 -0.16466667 0.05426751 0.01491765 FALSE TRUE
ENST00000462489 ENSG00000158435 HEK293_OSMI2_2hA HEK293_TMG_2hB CNOT11 protein_coding retained_intron 55.01405 51.90196 56.44222 6.167708 0.7902728 0.1209635 8.967302 0.6324478 10.65646 0.6324478 0.9271200 4.05335779 0.15106667 0.009866667 0.1888333 0.17896667 0.01491765 0.01491765 FALSE FALSE
MSTRG.18948.1 ENSG00000158435 HEK293_OSMI2_2hA HEK293_TMG_2hB CNOT11 protein_coding   55.01405 51.90196 56.44222 6.167708 0.7902728 0.1209635 3.223033 2.7487737 3.78614 0.3757092 0.5516447 0.46050616 0.06138333 0.053766667 0.0669000 0.01313333 0.68859927 0.01491765 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000158435 E001 12.446713 0.0015295922 3.253319e-01 4.687680e-01 2 101252795 101252885 91 + 1.154 1.107 -0.167
ENSG00000158435 E002 21.008304 0.0128765144 5.486332e-02 1.177609e-01 2 101252886 101252901 16 + 1.408 1.273 -0.469
ENSG00000158435 E003 328.799152 0.0200302948 2.304960e-02 5.785134e-02 2 101252902 101253478 577 + 2.576 2.459 -0.393
ENSG00000158435 E004 119.686640 0.0105980554 6.019606e-02 1.269427e-01 2 101257791 101257837 47 + 2.116 2.044 -0.240
ENSG00000158435 E005 273.051556 0.0033096458 8.235533e-04 3.343614e-03 2 101257838 101257955 118 + 2.474 2.402 -0.237
ENSG00000158435 E006 1.946739 0.0093306695 8.651583e-01 9.171273e-01 2 101262431 101262538 108 + 0.468 0.472 0.020
ENSG00000158435 E007 434.800824 0.0018364345 2.631987e-06 1.995271e-05 2 101262539 101262691 153 + 2.677 2.603 -0.248
ENSG00000158435 E008 475.665544 0.0006453649 2.058458e-07 1.968246e-06 2 101264845 101264974 130 + 2.706 2.655 -0.171
ENSG00000158435 E009 371.955570 0.0004804762 7.553917e-04 3.098956e-03 2 101264975 101265047 73 + 2.584 2.566 -0.060
ENSG00000158435 E010 567.962688 0.0001715530 1.347072e-01 2.406945e-01 2 101266677 101266879 203 + 2.742 2.768 0.088
ENSG00000158435 E011 352.494073 0.0036170395 3.365808e-02 7.907467e-02 2 101269040 101269136 97 + 2.482 2.596 0.382
ENSG00000158435 E012 51.646580 0.0285780441 8.362959e-01 8.976264e-01 2 101269137 101269215 79 + 1.707 1.730 0.080
ENSG00000158435 E013 1621.205442 0.0052656648 1.032546e-07 1.046002e-06 2 101269216 101270316 1101 + 3.101 3.282 0.602
ENSG00000158435 E014 10.844303 0.0976856273 3.331880e-02 7.842442e-02 2 101273500 101288876 15377 + 0.811 1.206 1.461