ENSG00000158321

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000418686 ENSG00000158321 HEK293_OSMI2_2hA HEK293_TMG_2hB AUTS2 protein_coding processed_transcript 14.57974 11.78059 15.38232 0.6798958 0.8218993 0.3845745 1.3943930 4.1363473 0.05349329 0.30547024 0.05349329 -6.029093 0.11140000 0.35580000 0.00360000 -0.35220000 2.124007e-07 2.124007e-07 FALSE TRUE
ENST00000464768 ENSG00000158321 HEK293_OSMI2_2hA HEK293_TMG_2hB AUTS2 protein_coding retained_intron 14.57974 11.78059 15.38232 0.6798958 0.8218993 0.3845745 0.8461332 0.2600392 1.21312076 0.08984444 0.03819442 2.179326 0.05480833 0.02226667 0.07903333 0.05676667 7.256596e-04 2.124007e-07 FALSE FALSE
ENST00000465899 ENSG00000158321 HEK293_OSMI2_2hA HEK293_TMG_2hB AUTS2 protein_coding retained_intron 14.57974 11.78059 15.38232 0.6798958 0.8218993 0.3845745 0.8022098 0.3413245 0.88030280 0.08893595 0.24862469 1.341492 0.05239167 0.02940000 0.05760000 0.02820000 4.365077e-01 2.124007e-07   FALSE
ENST00000644359 ENSG00000158321 HEK293_OSMI2_2hA HEK293_TMG_2hB AUTS2 protein_coding protein_coding 14.57974 11.78059 15.38232 0.6798958 0.8218993 0.3845745 1.5579835 0.8496096 2.36308848 0.29318705 0.30216254 1.465012 0.10739167 0.07093333 0.15263333 0.08170000 1.129839e-01 2.124007e-07 FALSE TRUE
ENST00000644949 ENSG00000158321 HEK293_OSMI2_2hA HEK293_TMG_2hB AUTS2 protein_coding protein_coding 14.57974 11.78059 15.38232 0.6798958 0.8218993 0.3845745 0.4609433 0.0000000 1.06873278 0.00000000 0.75902371 6.753194 0.02722917 0.00000000 0.06476667 0.06476667 2.364547e-01 2.124007e-07 FALSE FALSE
MSTRG.29991.17 ENSG00000158321 HEK293_OSMI2_2hA HEK293_TMG_2hB AUTS2 protein_coding   14.57974 11.78059 15.38232 0.6798958 0.8218993 0.3845745 1.7436417 0.4178750 1.61925335 0.41787502 0.20433933 1.928950 0.11256250 0.03966667 0.10603333 0.06636667 1.985973e-01 2.124007e-07 FALSE TRUE
MSTRG.29991.18 ENSG00000158321 HEK293_OSMI2_2hA HEK293_TMG_2hB AUTS2 protein_coding   14.57974 11.78059 15.38232 0.6798958 0.8218993 0.3845745 2.3076556 2.3223620 2.58371993 0.68173785 0.33785378 0.153231 0.15281250 0.19170000 0.17113333 -0.02056667 9.394636e-01 2.124007e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000158321 E001 0.5975289 0.0239560532 5.144070e-02 1.117143e-01 7 69598296 69598474 179 + 0.316 0.000 -16.874
ENSG00000158321 E002 13.1999074 0.0013402828 7.707966e-01 8.520396e-01 7 69598475 69598918 444 + 1.088 1.216 0.458
ENSG00000158321 E003 34.0975348 0.0046277279 9.753621e-01 9.886434e-01 7 69598919 69599610 692 + 1.483 1.589 0.363
ENSG00000158321 E004 15.2706930 0.0077829254 4.256378e-01 5.685505e-01 7 69599611 69599874 264 + 1.189 1.217 0.099
ENSG00000158321 E005 11.9599731 0.0078669888 2.541332e-03 8.886571e-03 7 69599875 69599962 88 + 1.189 0.970 -0.795
ENSG00000158321 E006 0.0000000       7 69643303 69643367 65 +      
ENSG00000158321 E007 0.0000000       7 69663166 69663264 99 +      
ENSG00000158321 E008 0.0000000       7 69824571 69824687 117 +      
ENSG00000158321 E009 0.2214452 0.0369247138 4.277847e-01   7 69825780 69825875 96 + 0.000 0.145 17.336
ENSG00000158321 E010 28.6232461 0.0008683023 8.903317e-01 9.339409e-01 7 69899286 69899498 213 + 1.418 1.514 0.333
ENSG00000158321 E011 0.0000000       7 69967464 69967804 341 +      
ENSG00000158321 E012 0.1472490 0.0426383884 5.530246e-01   7 70093232 70093303 72 + 0.103 0.000 -17.293
ENSG00000158321 E013 23.4988455 0.0008307103 8.034701e-01 8.749094e-01 7 70118132 70118233 102 + 1.340 1.427 0.302
ENSG00000158321 E014 4.6403557 0.0037057969 1.379641e-01 2.452418e-01 7 70118234 70120370 2137 + 0.596 0.888 1.193
ENSG00000158321 E015 14.9084915 0.0020416295 8.725066e-01 9.220055e-01 7 70134536 70134555 20 + 1.158 1.246 0.314
ENSG00000158321 E016 14.6494955 0.0200231388 8.014260e-01 8.734870e-01 7 70134556 70134571 16 + 1.158 1.236 0.277
ENSG00000158321 E017 0.2955422 0.0293948735 9.191743e-01   7 70145306 70145444 139 + 0.103 0.145 0.562
ENSG00000158321 E018 0.0000000       7 70194669 70194820 152 +      
ENSG00000158321 E019 20.0230073 0.0364131857 6.876356e-02 1.413173e-01 7 70290368 70294239 3872 + 1.150 1.474 1.134
ENSG00000158321 E020 0.1472490 0.0426383884 5.530246e-01   7 70316916 70317046 131 + 0.103 0.000 -17.293
ENSG00000158321 E021 6.2463465 0.0026651789 2.830738e-03 9.766079e-03 7 70435752 70435781 30 + 0.956 0.659 -1.171
ENSG00000158321 E022 0.0000000       7 70435782 70437858 2077 +      
ENSG00000158321 E023 5.9690718 0.0027517037 7.976836e-01 8.709212e-01 7 70596085 70596799 715 + 0.784 0.910 0.486
ENSG00000158321 E024 25.5513862 0.0302214373 9.610438e-04 3.825255e-03 7 70694058 70694786 729 + 1.509 1.265 -0.846
ENSG00000158321 E025 18.2537191 0.0364488607 5.405773e-02 1.163560e-01 7 70694787 70694852 66 + 1.334 1.208 -0.444
ENSG00000158321 E026 17.9417957 0.0083055290 3.957316e-04 1.758088e-03 7 70694853 70694872 20 + 1.360 1.136 -0.790
ENSG00000158321 E027 56.1056314 0.0006892810 1.516668e-07 1.487668e-06 7 70694873 70695045 173 + 1.811 1.664 -0.496
ENSG00000158321 E028 0.1472490 0.0426383884 5.530246e-01   7 70698567 70698568 2 + 0.103 0.000 -17.293
ENSG00000158321 E029 49.3423331 0.0006170569 2.479993e-02 6.146527e-02 7 70698569 70698620 52 + 1.699 1.693 -0.020
ENSG00000158321 E030 0.4782907 0.0239405729 6.491978e-02 1.349003e-01 7 70698621 70699490 870 + 0.000 0.339 18.997
ENSG00000158321 E031 0.0000000       7 70729139 70729221 83 +      
ENSG00000158321 E032 0.5891098 0.0184215816 5.065026e-02 1.103139e-01 7 70738590 70738716 127 + 0.316 0.000 -19.485
ENSG00000158321 E033 111.1689185 0.0029266619 2.061311e-03 7.415510e-03 7 70762870 70763283 414 + 2.049 2.033 -0.056
ENSG00000158321 E034 25.7891399 0.0082858523 3.747383e-01 5.191675e-01 7 70763284 70763341 58 + 1.400 1.437 0.128
ENSG00000158321 E035 4.4938914 0.1265684379 7.215480e-01 8.158661e-01 7 70764752 70764754 3 + 0.724 0.735 0.046
ENSG00000158321 E036 27.0661212 0.0105023917 2.768962e-01 4.163894e-01 7 70764755 70764930 176 + 1.431 1.449 0.065
ENSG00000158321 E037 25.9270438 0.0040372432 2.453079e-02 6.091125e-02 7 70764931 70765005 75 + 1.447 1.388 -0.206
ENSG00000158321 E038 37.8234083 0.0039071655 1.725035e-02 4.550176e-02 7 70766114 70766233 120 + 1.600 1.556 -0.149
ENSG00000158321 E039 50.2601210 0.0049608024 2.295441e-03 8.136063e-03 7 70766234 70766334 101 + 1.732 1.660 -0.246
ENSG00000158321 E040 13.9594477 0.0018230489 1.070664e-01 2.012082e-01 7 70766335 70766344 10 + 1.181 1.136 -0.161
ENSG00000158321 E041 15.0094320 0.0082153528 7.540396e-02 1.521662e-01 7 70766345 70766355 11 + 1.218 1.151 -0.238
ENSG00000158321 E042 31.1960628 0.0007312770 6.578000e-02 1.363349e-01 7 70768024 70768068 45 + 1.502 1.493 -0.032
ENSG00000158321 E043 0.4427912 0.1485789765 6.837052e-01 7.868452e-01 7 70771216 70771446 231 + 0.186 0.144 -0.447
ENSG00000158321 E044 1.2072627 0.0293858434 2.277325e-01 3.597009e-01 7 70771447 70771548 102 + 0.416 0.253 -1.021
ENSG00000158321 E045 63.9803914 0.0004530627 2.148954e-03 7.687032e-03 7 70771549 70771644 96 + 1.818 1.790 -0.096
ENSG00000158321 E046 48.4440997 0.0057998469 9.363620e-03 2.719136e-02 7 70774028 70774099 72 + 1.712 1.661 -0.173
ENSG00000158321 E047 6.5876066 0.0167656672 7.933562e-03 2.361186e-02 7 70774100 70774376 277 + 0.968 0.698 -1.057
ENSG00000158321 E048 7.5256324 0.0021972779 1.076317e-05 7.099262e-05 7 70774787 70775143 357 + 1.078 0.620 -1.792
ENSG00000158321 E049 6.9979447 0.0024153089 2.630614e-03 9.161572e-03 7 70775144 70775356 213 + 1.004 0.729 -1.063
ENSG00000158321 E050 36.5208266 0.0098873276 2.124619e-02 5.410433e-02 7 70775357 70775361 5 + 1.594 1.533 -0.210
ENSG00000158321 E051 40.8636561 0.0086121806 1.091253e-02 3.095291e-02 7 70775362 70775386 25 + 1.644 1.576 -0.232
ENSG00000158321 E052 8.5764955 0.0061126340 1.185391e-02 3.318195e-02 7 70776674 70777102 429 + 1.058 0.865 -0.722
ENSG00000158321 E053 65.3287008 0.0013679522 1.940917e-03 7.043267e-03 7 70777103 70777174 72 + 1.828 1.793 -0.119
ENSG00000158321 E054 0.7374030 0.0157241610 2.218966e-01 3.526590e-01 7 70777175 70777217 43 + 0.316 0.145 -1.436
ENSG00000158321 E055 0.7353267 0.1189545915 2.637988e-01 4.017194e-01 7 70781470 70781614 145 + 0.316 0.144 -1.443
ENSG00000158321 E056 36.3770760 0.0006195667 3.900544e-04 1.736246e-03 7 70781615 70781620 6 + 1.606 1.504 -0.348
ENSG00000158321 E057 84.6916721 0.0003426754 5.667771e-05 3.149913e-04 7 70781621 70781756 136 + 1.947 1.900 -0.158
ENSG00000158321 E058 2.8400366 0.0058622837 9.640678e-01 9.813380e-01 7 70781757 70784742 2986 + 0.533 0.620 0.396
ENSG00000158321 E059 69.4322820 0.0004067587 5.245225e-03 1.659805e-02 7 70784942 70785019 78 + 1.845 1.835 -0.036
ENSG00000158321 E060 0.5202097 0.0202164455 6.508642e-01 7.618513e-01 7 70785409 70785472 64 + 0.186 0.145 -0.433
ENSG00000158321 E061 73.0344863 0.0005003841 4.842653e-03 1.549326e-02 7 70785955 70786038 84 + 1.866 1.857 -0.032
ENSG00000158321 E062 2.5956625 0.0137352213 8.283395e-01 8.921242e-01 7 70786921 70787085 165 + 0.533 0.576 0.202
ENSG00000158321 E063 2.4784887 0.1038451923 6.378273e-01 7.516218e-01 7 70787086 70787137 52 + 0.459 0.620 0.756
ENSG00000158321 E064 2.8096322 0.0056543416 6.009457e-01 7.221251e-01 7 70787138 70787208 71 + 0.497 0.659 0.735
ENSG00000158321 E065 106.9359168 0.0045853786 1.585509e-01 2.731902e-01 7 70787209 70787431 223 + 2.003 2.051 0.160
ENSG00000158321 E066 1035.5527155 0.0082320618 2.352600e-11 4.614756e-10 7 70789748 70793506 3759 + 2.853 3.152 0.994