ENSG00000158161

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000373871 ENSG00000158161 HEK293_OSMI2_2hA HEK293_TMG_2hB EYA3 protein_coding protein_coding 23.84267 20.21627 26.61846 1.372367 1.005193 0.3967386 4.682322 3.9023959 5.295829 0.8019178 0.2161163 0.4395258 0.20102917 0.18940000 0.19920000 0.00980000 0.92240729 0.0004891561 FALSE  
ENST00000495923 ENSG00000158161 HEK293_OSMI2_2hA HEK293_TMG_2hB EYA3 protein_coding processed_transcript 23.84267 20.21627 26.61846 1.372367 1.005193 0.3967386 1.486612 0.6096823 2.693735 0.3826753 0.9092820 2.1253530 0.05774583 0.02996667 0.09910000 0.06913333 0.25862291 0.0004891561 FALSE  
ENST00000540618 ENSG00000158161 HEK293_OSMI2_2hA HEK293_TMG_2hB EYA3 protein_coding protein_coding 23.84267 20.21627 26.61846 1.372367 1.005193 0.3967386 14.635212 13.6317122 15.440280 0.7687137 0.1369322 0.1796082 0.61471667 0.67653333 0.58186667 -0.09466667 0.19004603 0.0004891561 FALSE  
MSTRG.674.8 ENSG00000158161 HEK293_OSMI2_2hA HEK293_TMG_2hB EYA3 protein_coding   23.84267 20.21627 26.61846 1.372367 1.005193 0.3967386 1.399266 0.3342976 1.870047 0.1189525 0.2679620 2.4490405 0.05447500 0.01680000 0.07113333 0.05433333 0.01564576 0.0004891561 TRUE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000158161 E001 1.8089511 0.0430613858 4.399156e-04 1.927799e-03 1 27970344 27970346 3 - 0.000 0.662 11.849
ENSG00000158161 E002 119.7250325 0.0234416679 1.516919e-06 1.211669e-05 1 27970347 27970435 89 - 1.805 2.254 1.506
ENSG00000158161 E003 520.3564314 0.0125940903 7.810788e-14 2.256719e-12 1 27970436 27970787 352 - 2.440 2.887 1.489
ENSG00000158161 E004 1792.3341858 0.0003926100 1.206898e-46 6.113457e-44 1 27970788 27973467 2680 - 3.159 3.335 0.588
ENSG00000158161 E005 484.5376203 0.0030181781 1.713282e-03 6.323814e-03 1 27973468 27974546 1079 - 2.710 2.661 -0.164
ENSG00000158161 E006 3.1460637 0.0052064620 1.146717e-01 2.123538e-01 1 27977273 27977389 117 - 0.710 0.499 -0.937
ENSG00000158161 E007 110.4319964 0.0004203130 4.028098e-06 2.927723e-05 1 27978374 27978474 101 - 2.100 1.985 -0.386
ENSG00000158161 E008 0.6664265 0.0165733714 9.230069e-01 9.554456e-01 1 27988473 27988478 6 - 0.201 0.235 0.286
ENSG00000158161 E009 5.0840908 0.1253312179 3.521217e-01 4.963719e-01 1 27988479 27988534 56 - 0.656 0.900 0.978
ENSG00000158161 E010 136.1177458 0.0037378616 7.940158e-03 2.362927e-02 1 27988535 27988656 122 - 2.171 2.101 -0.235
ENSG00000158161 E011 147.9650968 0.0075156327 2.479966e-02 6.146527e-02 1 27989697 27989811 115 - 2.209 2.136 -0.244
ENSG00000158161 E012 173.5848600 0.0002833741 1.673244e-08 1.978350e-07 1 27993400 27993560 161 - 2.294 2.183 -0.371
ENSG00000158161 E013 130.7897454 0.0042400021 1.750047e-06 1.378893e-05 1 27997320 27997378 59 - 2.195 2.024 -0.575
ENSG00000158161 E014 11.0279121 0.0098909317 3.941772e-01 5.380635e-01 1 27998284 27998351 68 - 1.107 1.057 -0.182
ENSG00000158161 E015 146.1601659 0.0002730780 4.096393e-12 9.084037e-11 1 27999960 28000049 90 - 2.242 2.079 -0.546
ENSG00000158161 E016 136.7914258 0.0002702881 6.359350e-15 2.157671e-13 1 28004336 28004419 84 - 2.226 2.028 -0.661
ENSG00000158161 E017 0.5922303 0.0179945086 3.599344e-01 5.042446e-01 1 28010796 28010946 151 - 0.275 0.133 -1.301
ENSG00000158161 E018 142.0644585 0.0057536500 1.862356e-06 1.459130e-05 1 28010947 28011083 137 - 2.236 2.052 -0.618
ENSG00000158161 E019 56.9624370 0.0121150259 5.401555e-04 2.310473e-03 1 28011084 28011086 3 - 1.853 1.649 -0.689
ENSG00000158161 E020 139.7256977 0.0061567308 1.695220e-04 8.330206e-04 1 28013111 28013294 184 - 2.212 2.075 -0.460
ENSG00000158161 E021 102.5794634 0.0070551794 1.352087e-04 6.821625e-04 1 28017154 28017239 86 - 2.089 1.931 -0.531
ENSG00000158161 E022 49.2595991 0.0034707712 3.629989e-05 2.113370e-04 1 28027789 28027926 138 - 1.790 1.599 -0.649
ENSG00000158161 E023 159.3130338 0.0078126198 1.737388e-07 1.685838e-06 1 28035544 28035680 137 - 2.303 2.082 -0.737
ENSG00000158161 E024 112.9199103 0.0080205509 4.067246e-06 2.952589e-05 1 28038839 28038905 67 - 2.151 1.942 -0.699
ENSG00000158161 E025 110.4313688 0.0085399853 3.444283e-07 3.147808e-06 1 28042571 28042650 80 - 2.153 1.908 -0.823
ENSG00000158161 E026 97.5145953 0.0085939655 1.971959e-04 9.518004e-04 1 28048383 28048426 44 - 2.072 1.896 -0.589
ENSG00000158161 E027 115.5628922 0.0186500879 6.322709e-03 1.946375e-02 1 28057994 28058094 101 - 2.139 1.978 -0.539
ENSG00000158161 E028 8.9616095 0.0393981543 3.127105e-02 7.442939e-02 1 28080332 28080451 120 - 1.107 0.834 -1.019
ENSG00000158161 E029 80.1393046 0.0180857819 2.703225e-03 9.383917e-03 1 28088524 28088688 165 - 1.991 1.805 -0.624