ENSG00000157916

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000378518 ENSG00000157916 HEK293_OSMI2_2hA HEK293_TMG_2hB RER1 protein_coding protein_coding 155.7879 203.6019 128.6789 6.710337 0.8450551 -0.6619342 30.06049 33.34549 28.146065 4.4560982 1.2103615 -0.2444787 0.19085417 0.16270000 0.21870000 0.05600000 0.166979885 0.0001325434 FALSE FALSE
ENST00000443438 ENSG00000157916 HEK293_OSMI2_2hA HEK293_TMG_2hB RER1 protein_coding protein_coding 155.7879 203.6019 128.6789 6.710337 0.8450551 -0.6619342 75.99936 109.82907 58.202405 2.5784637 1.4914502 -0.9159928 0.48739167 0.53983333 0.45236667 -0.08746667 0.001901174 0.0001325434 FALSE FALSE
ENST00000605895 ENSG00000157916 HEK293_OSMI2_2hA HEK293_TMG_2hB RER1 protein_coding protein_coding 155.7879 203.6019 128.6789 6.710337 0.8450551 -0.6619342 16.47680 27.13989 11.951342 1.9957206 0.5292754 -1.1825671 0.10429167 0.13390000 0.09283333 -0.04106667 0.067236398 0.0001325434 FALSE TRUE
MSTRG.144.3 ENSG00000157916 HEK293_OSMI2_2hA HEK293_TMG_2hB RER1 protein_coding   155.7879 203.6019 128.6789 6.710337 0.8450551 -0.6619342 8.93683 10.51982 8.111621 0.7588997 0.5003967 -0.3746406 0.05619583 0.05156667 0.06296667 0.01140000 0.264769160 0.0001325434 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000157916 E001 1.868488 2.039049e-01 6.914191e-01 7.929012e-01 1 2391775 2391786 12 + 0.357 0.451 0.519
ENSG00000157916 E002 3.162633 2.846564e-02 5.937407e-01 7.160698e-01 1 2391787 2391793 7 + 0.644 0.546 -0.438
ENSG00000157916 E003 45.061613 1.408439e-02 2.406797e-03 8.482354e-03 1 2391794 2391827 34 + 1.779 1.534 -0.834
ENSG00000157916 E004 86.706656 8.306433e-03 7.511734e-03 2.254413e-02 1 2391828 2391840 13 + 2.017 1.850 -0.561
ENSG00000157916 E005 89.991560 8.178902e-03 5.118455e-03 1.625181e-02 1 2391841 2391842 2 + 2.036 1.864 -0.579
ENSG00000157916 E006 138.637436 8.421598e-03 4.764611e-04 2.070088e-03 1 2391843 2391856 14 + 2.239 2.040 -0.665
ENSG00000157916 E007 299.391041 1.135499e-02 1.631985e-02 4.343193e-02 1 2391857 2391889 33 + 2.538 2.398 -0.470
ENSG00000157916 E008 498.296706 4.409415e-03 2.877850e-02 6.955774e-02 1 2391890 2391958 69 + 2.727 2.639 -0.293
ENSG00000157916 E009 8.269331 3.585207e-02 1.633933e-01 2.795861e-01 1 2392039 2392126 88 + 1.066 0.860 -0.769
ENSG00000157916 E010 27.641161 1.121332e-02 4.979449e-02 1.087952e-01 1 2392259 2392375 117 + 1.534 1.358 -0.606
ENSG00000157916 E011 44.836538 7.924576e-03 6.836643e-06 4.719877e-05 1 2392457 2392562 106 + 1.817 1.501 -1.073
ENSG00000157916 E012 66.449683 9.323052e-03 4.919326e-06 3.506345e-05 1 2392563 2392715 153 + 1.980 1.680 -1.012
ENSG00000157916 E013 72.757446 3.414177e-04 4.940519e-17 2.201756e-15 1 2392991 2393157 167 + 2.048 1.690 -1.204
ENSG00000157916 E014 34.959807 6.028462e-04 3.135319e-06 2.335549e-05 1 2393174 2393207 34 + 1.698 1.413 -0.975
ENSG00000157916 E015 56.214236 6.059677e-04 4.311671e-05 2.467029e-04 1 2393208 2393231 24 + 1.858 1.651 -0.698
ENSG00000157916 E016 60.544834 8.154724e-03 1.170568e-03 4.540927e-03 1 2393232 2393269 38 + 1.895 1.683 -0.716
ENSG00000157916 E017 188.603940 7.871614e-04 1.774180e-04 8.671871e-04 1 2393270 2393355 86 + 2.327 2.206 -0.403
ENSG00000157916 E018 82.107543 2.130086e-02 1.016899e-06 8.419947e-06 1 2394005 2394646 642 + 2.117 1.710 -1.368
ENSG00000157916 E019 27.629941 1.716902e-02 6.189204e-03 1.911223e-02 1 2394647 2394728 82 + 1.580 1.321 -0.895
ENSG00000157916 E020 107.094406 1.820821e-02 9.664943e-06 6.448526e-05 1 2394729 2395607 879 + 2.201 1.860 -1.144
ENSG00000157916 E021 19.523177 9.294112e-04 1.957671e-03 7.093805e-03 1 2395608 2395648 41 + 1.437 1.189 -0.870
ENSG00000157916 E022 29.016409 6.784499e-04 6.940219e-07 5.963911e-06 1 2395649 2395783 135 + 1.643 1.315 -1.128
ENSG00000157916 E023 887.844030 1.106060e-04 4.414794e-01 5.831214e-01 1 2395784 2395871 88 + 2.935 2.914 -0.070
ENSG00000157916 E024 8.549422 4.997325e-03 3.030068e-03 1.035440e-02 1 2395872 2396367 496 + 1.152 0.811 -1.269
ENSG00000157916 E025 1090.654954 7.553703e-05 3.239788e-06 2.406368e-05 1 2397116 2397220 105 + 2.981 3.023 0.140
ENSG00000157916 E026 1247.154044 1.163709e-04 3.187341e-10 5.167749e-09 1 2399415 2399514 100 + 3.027 3.086 0.195
ENSG00000157916 E027 1253.704247 8.285091e-05 1.183568e-10 2.064292e-09 1 2400857 2400935 79 + 3.030 3.088 0.194
ENSG00000157916 E028 13.810832 6.898487e-03 4.593178e-01 5.995109e-01 1 2401951 2402096 146 + 1.201 1.120 -0.291
ENSG00000157916 E029 35.206180 8.211642e-03 2.296306e-01 3.619987e-01 1 2402097 2402206 110 + 1.476 1.559 0.283
ENSG00000157916 E030 1620.730224 1.439646e-04 1.777487e-10 3.005530e-09 1 2402207 2402342 136 + 3.146 3.199 0.177
ENSG00000157916 E031 3201.178464 2.375039e-03 3.991561e-01 5.428868e-01 1 2403035 2405442 2408 + 3.474 3.479 0.017