ENSG00000157869

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000288723 ENSG00000157869 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB28 protein_coding protein_coding 9.293579 6.420416 14.3558 1.390746 0.3882343 1.159655 1.6245114 0.9943853 3.1018949 0.3659808 0.27447770 1.6314805 0.15421667 0.1446667 0.21540000 0.07073333 0.29642846 0.02043133 FALSE TRUE
ENST00000330852 ENSG00000157869 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB28 protein_coding protein_coding 9.293579 6.420416 14.3558 1.390746 0.3882343 1.159655 5.1705959 2.6007370 8.6642018 0.9158789 0.34949107 1.7322739 0.51138750 0.3847667 0.60416667 0.21940000 0.02043133 0.02043133 FALSE TRUE
ENST00000508274 ENSG00000157869 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB28 protein_coding nonsense_mediated_decay 9.293579 6.420416 14.3558 1.390746 0.3882343 1.159655 0.6473730 0.8040964 1.0153288 0.4798298 0.09211615 0.3328150 0.08660417 0.1515667 0.07056667 -0.08100000 1.00000000 0.02043133 TRUE FALSE
MSTRG.24631.1 ENSG00000157869 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB28 protein_coding   9.293579 6.420416 14.3558 1.390746 0.3882343 1.159655 0.5778685 0.6627022 0.5704839 0.1446184 0.19255215 -0.2127117 0.09527500 0.1030667 0.04023333 -0.06283333 0.11739830 0.02043133 FALSE TRUE
MSTRG.24631.2 ENSG00000157869 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB28 protein_coding   9.293579 6.420416 14.3558 1.390746 0.3882343 1.159655 0.6623321 0.7910287 0.4943940 0.1871790 0.23372952 -0.6673029 0.08621667 0.1272667 0.03406667 -0.09320000 0.06833511 0.02043133 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000157869 E001 4.4146692 0.0038751587 0.0001014608 5.286263e-04 4 12972932 12973049 118 - 0.463 1.000 2.237
ENSG00000157869 E002 0.2987644 0.0275567523 0.6138188932   4 13337164 13337503 340 - 0.153 0.000 -9.986
ENSG00000157869 E003 0.4804688 0.0220650371 0.9090720021 9.462454e-01 4 13342239 13342312 74 - 0.153 0.176 0.238
ENSG00000157869 E004 0.4772466 0.0209511444 0.1907055034 3.146377e-01 4 13361354 13361633 280 - 0.083 0.301 2.237
ENSG00000157869 E005 0.8480291 0.0147116557 0.0010643645 4.181941e-03 4 13367724 13367724 1 - 0.000 0.544 12.827
ENSG00000157869 E006 175.0568333 0.0002988406 0.0000128777 8.338942e-05 4 13367725 13368500 776 - 2.190 2.299 0.363
ENSG00000157869 E007 94.9585715 0.0003643395 0.9620721846 9.800860e-01 4 13368501 13368650 150 - 1.964 1.968 0.012
ENSG00000157869 E008 17.9237039 0.0019787511 0.7853259905 8.623916e-01 4 13369871 13369965 95 - 1.254 1.278 0.084
ENSG00000157869 E009 3.1074702 0.0053212786 0.2579466720 3.951421e-01 4 13371795 13371867 73 - 0.663 0.477 -0.851
ENSG00000157869 E010 64.6465947 0.0005910781 0.2490630839 3.848069e-01 4 13376545 13376622 78 - 1.817 1.767 -0.171
ENSG00000157869 E011 78.3862579 0.0003369254 0.0030290366 1.035162e-02 4 13381491 13381594 104 - 1.920 1.799 -0.409
ENSG00000157869 E012 0.6234634 0.0172705333 0.0665532945 1.376439e-01 4 13459863 13459920 58 - 0.083 0.397 2.821
ENSG00000157869 E013 72.4172976 0.0055985331 0.3342026810 4.779619e-01 4 13460699 13460828 130 - 1.867 1.819 -0.162
ENSG00000157869 E014 65.4397486 0.0003823822 0.1875624899 3.106778e-01 4 13474318 13474406 89 - 1.824 1.767 -0.193
ENSG00000157869 E015 61.7149121 0.0006922267 0.0145419784 3.945936e-02 4 13479430 13479526 97 - 1.814 1.698 -0.392
ENSG00000157869 E016 62.0758943 0.0021328959 0.5036668298 6.396391e-01 4 13484076 13484374 299 - 1.773 1.810 0.126