ENSG00000157827

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000288670 ENSG00000157827 HEK293_OSMI2_2hA HEK293_TMG_2hB FMNL2 protein_coding protein_coding 7.451392 3.42847 10.31829 0.3838478 0.2243552 1.586762 0.9933164 0.1647421 0.9220290 0.05803976 0.4768306 2.415148 0.1145458 0.04876667 0.08856667 0.0398000 8.014702e-01 6.144779e-05 FALSE TRUE
ENST00000475377 ENSG00000157827 HEK293_OSMI2_2hA HEK293_TMG_2hB FMNL2 protein_coding protein_coding 7.451392 3.42847 10.31829 0.3838478 0.2243552 1.586762 1.1805824 1.8012434 0.4438529 0.39684026 0.2303809 -1.996684 0.2480500 0.52416667 0.04213333 -0.4820333 6.144779e-05 6.144779e-05 FALSE TRUE
MSTRG.19342.2 ENSG00000157827 HEK293_OSMI2_2hA HEK293_TMG_2hB FMNL2 protein_coding   7.451392 3.42847 10.31829 0.3838478 0.2243552 1.586762 2.9157359 1.1844911 5.0896438 0.25886491 0.2322112 2.094000 0.3835750 0.35303333 0.49426667 0.1412333 3.915690e-01 6.144779e-05 FALSE TRUE
MSTRG.19342.3 ENSG00000157827 HEK293_OSMI2_2hA HEK293_TMG_2hB FMNL2 protein_coding   7.451392 3.42847 10.31829 0.3838478 0.2243552 1.586762 2.3033511 0.2779937 3.8371504 0.14518404 0.1582363 3.739681 0.2408333 0.07396667 0.37263333 0.2986667 1.762639e-04 6.144779e-05 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000157827 E001 2.6737620 0.0840480713 6.036415e-01 7.243812e-01 2 152335174 152335190 17 + 0.478 0.621 0.664
ENSG00000157827 E002 26.5156363 0.0011093097 1.035068e-02 2.962852e-02 2 152335191 152335577 387 + 1.405 1.257 -0.516
ENSG00000157827 E003 24.1891097 0.0209096433 3.166801e-02 7.520129e-02 2 152335578 152335720 143 + 1.372 1.194 -0.626
ENSG00000157827 E004 25.8810016 0.0008077257 9.774113e-05 5.114563e-04 2 152521943 152522026 84 + 1.417 1.135 -0.988
ENSG00000157827 E005 31.9113548 0.0006250635 2.297513e-04 1.090815e-03 2 152542739 152542819 81 + 1.496 1.272 -0.774
ENSG00000157827 E006 30.3476039 0.0045879595 2.547183e-04 1.194890e-03 2 152549021 152549097 77 + 1.479 1.226 -0.879
ENSG00000157827 E007 28.1827370 0.0504341978 3.778607e-03 1.252179e-02 2 152558740 152558823 84 + 1.459 1.138 -1.122
ENSG00000157827 E008 31.5312760 0.0628010845 2.800394e-03 9.676191e-03 2 152560883 152561035 153 + 1.510 1.159 -1.224
ENSG00000157827 E009 32.8139407 0.0006429683 3.535857e-06 2.605435e-05 2 152575136 152575244 109 + 1.518 1.209 -1.074
ENSG00000157827 E010 0.2966881 0.0270187286 7.843659e-01   2 152578621 152578887 267 + 0.125 0.000 -9.392
ENSG00000157827 E011 34.3797549 0.0006931447 1.156829e-05 7.576232e-05 2 152578888 152578964 77 + 1.533 1.257 -0.956
ENSG00000157827 E012 40.3732096 0.0005162467 5.318684e-05 2.976951e-04 2 152580956 152581049 94 + 1.593 1.376 -0.744
ENSG00000157827 E013 38.6077019 0.0006012905 1.702363e-03 6.290744e-03 2 152607339 152607413 75 + 1.562 1.410 -0.524
ENSG00000157827 E014 0.1515154 0.0429616589 1.000000e+00   2 152607414 152607653 240 + 0.067 0.000 -8.393
ENSG00000157827 E015 41.3191805 0.0004949732 2.154970e-03 7.704874e-03 2 152611495 152611605 111 + 1.589 1.450 -0.475
ENSG00000157827 E016 50.2887022 0.0004812479 9.346193e-04 3.735235e-03 2 152614851 152615000 150 + 1.672 1.538 -0.457
ENSG00000157827 E017 50.6825574 0.0005901032 1.393953e-02 3.808649e-02 2 152617091 152617192 102 + 1.663 1.584 -0.270
ENSG00000157827 E018 58.9510927 0.0004836302 3.959636e-03 1.302985e-02 2 152618846 152619158 313 + 1.730 1.638 -0.314
ENSG00000157827 E019 7.9791345 0.0021233362 6.925829e-02 1.421444e-01 2 152619509 152619555 47 + 0.931 0.735 -0.766
ENSG00000157827 E020 1.8863878 0.0306824084 4.706903e-01 6.099197e-01 2 152619556 152619577 22 + 0.369 0.548 0.917
ENSG00000157827 E021 20.5196221 0.0009130715 3.054179e-01 4.475181e-01 2 152619578 152619718 141 + 1.269 1.242 -0.095
ENSG00000157827 E022 0.0000000       2 152622537 152622557 21 +      
ENSG00000157827 E023 0.0000000       2 152625148 152625437 290 +      
ENSG00000157827 E024 43.1354912 0.0006172476 1.791032e-01 3.000210e-01 2 152625438 152625562 125 + 1.580 1.562 -0.062
ENSG00000157827 E025 76.7804716 0.0070718362 5.048685e-02 1.100313e-01 2 152626525 152626727 203 + 1.836 1.783 -0.180
ENSG00000157827 E026 88.6548713 0.0003147119 1.950593e-02 5.042502e-02 2 152628299 152628533 235 + 1.892 1.857 -0.119
ENSG00000157827 E027 47.9001659 0.0114211720 2.656260e-01 4.036970e-01 2 152629656 152629724 69 + 1.626 1.607 -0.063
ENSG00000157827 E028 45.2901154 0.0041579091 8.769138e-01 9.249876e-01 2 152629825 152629905 81 + 1.574 1.652 0.265
ENSG00000157827 E029 41.6162477 0.0005610597 4.875119e-01 6.251877e-01 2 152632008 152632137 130 + 1.554 1.576 0.076
ENSG00000157827 E030 60.2772404 0.0034642180 8.006633e-01 8.729678e-01 2 152636427 152636590 164 + 1.702 1.755 0.179
ENSG00000157827 E031 51.0847366 0.0008589495 3.218600e-01 4.651238e-01 2 152637573 152637674 102 + 1.613 1.734 0.411
ENSG00000157827 E032 60.3695083 0.0004116962 1.513206e-01 2.634663e-01 2 152639958 152640056 99 + 1.679 1.817 0.467
ENSG00000157827 E033 63.5481733 0.0004641091 3.190705e-01 4.621078e-01 2 152640791 152640914 124 + 1.709 1.825 0.390
ENSG00000157827 E034 1.8143566 0.0087225271 1.693931e-01 2.874008e-01 2 152643419 152643544 126 + 0.454 0.213 -1.541
ENSG00000157827 E035 34.9192789 0.0006409938 8.231155e-01 8.885133e-01 2 152645434 152645480 47 + 1.472 1.522 0.174
ENSG00000157827 E036 18.5264909 0.0009889923 9.355383e-01 9.634280e-01 2 152645481 152645609 129 + 1.201 1.272 0.251
ENSG00000157827 E037 85.1236837 0.0044230576 1.932854e-01 3.179029e-01 2 152647796 152648142 347 + 1.829 1.964 0.455
ENSG00000157827 E038 185.1449343 0.0067018313 3.907206e-06 2.847722e-05 2 152648143 152649000 858 + 2.119 2.390 0.906
ENSG00000157827 E039 149.5481958 0.0252316997 1.243559e-12 3.005338e-11 2 152649001 152650346 1346 + 1.866 2.481 2.057