ENSG00000157778

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000288607 ENSG00000157778 HEK293_OSMI2_2hA HEK293_TMG_2hB PSMG3 protein_coding protein_coding 199.3122 362.8855 114.0869 11.94761 2.08923 -1.669294 29.86457 46.77523 22.07017 1.556144 0.8091583 -1.083302 0.1616333 0.1289000 0.1933667 0.06446667 7.171053e-11 7.171053e-11 FALSE  
ENST00000404674 ENSG00000157778 HEK293_OSMI2_2hA HEK293_TMG_2hB PSMG3 protein_coding protein_coding 199.3122 362.8855 114.0869 11.94761 2.08923 -1.669294 160.20286 298.19623 86.14160 8.620750 1.2663125 -1.791361 0.7947500 0.8219667 0.7553000 -0.06666667 1.041497e-03 7.171053e-11 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000157778 E001 21.037621 1.064925e-03 3.123481e-01 4.548914e-01 7 1567332 1567337 6 - 1.170 1.270 0.354
ENSG00000157778 E002 60.166186 5.189696e-04 1.636515e-02 4.353171e-02 7 1567338 1567347 10 - 1.568 1.716 0.502
ENSG00000157778 E003 83.471843 8.099405e-04 4.386636e-01 5.805344e-01 7 1567348 1567350 3 - 1.794 1.838 0.150
ENSG00000157778 E004 108.208713 6.551108e-04 1.775657e-01 2.980845e-01 7 1567351 1567371 21 - 1.888 1.953 0.219
ENSG00000157778 E005 2139.450162 1.547571e-03 2.400135e-02 5.981458e-02 7 1567372 1567612 241 - 3.192 3.239 0.155
ENSG00000157778 E006 2942.981660 5.432297e-05 1.208107e-02 3.373010e-02 7 1567613 1567850 238 - 3.348 3.371 0.079
ENSG00000157778 E007 2201.693523 3.094055e-04 5.952086e-01 7.173287e-01 7 1569124 1569360 237 - 3.231 3.243 0.041
ENSG00000157778 E008 655.428789 1.496895e-04 4.108159e-24 4.120055e-22 7 1569361 1569847 487 - 2.836 2.679 -0.522
ENSG00000157778 E009 177.870252 7.775511e-03 2.386647e-07 2.253954e-06 7 1569848 1569998 151 - 2.343 2.086 -0.862
ENSG00000157778 E010 12.783108 1.467576e-03 3.433534e-04 1.553553e-03 7 1569999 1570008 10 - 1.292 0.943 -1.259
ENSG00000157778 E011 357.476338 2.441975e-03 1.699566e-02 4.492441e-02 7 1570009 1570045 37 - 2.380 2.467 0.291
ENSG00000157778 E012 41.245969 1.261846e-03 8.847410e-02 1.727900e-01 7 1570474 1570933 460 - 1.602 1.497 -0.359
ENSG00000157778 E013 10.511465 1.813864e-03 6.950507e-02 1.425366e-01 7 1570934 1570965 32 - 1.114 0.916 -0.729
ENSG00000157778 E014 9.001163 2.210934e-03 1.380468e-02 3.778343e-02 7 1570966 1573499 2534 - 1.114 0.834 -1.042