ENSG00000157654

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000374525 ENSG00000157654 HEK293_OSMI2_2hA HEK293_TMG_2hB PALM2AKAP2 protein_coding protein_coding 6.674974 4.818026 8.887592 0.3791428 0.3414699 0.8819813 4.2160180 3.4983016 4.8802714 0.17602230 0.3267144 0.4791418 0.65097083 0.72940000 0.5482000 -0.18120000 0.003100803 0.003100803 FALSE TRUE
ENST00000482335 ENSG00000157654 HEK293_OSMI2_2hA HEK293_TMG_2hB PALM2AKAP2 protein_coding processed_transcript 6.674974 4.818026 8.887592 0.3791428 0.3414699 0.8819813 1.3911065 0.7797964 2.0675480 0.20780680 0.1699325 1.3953291 0.19982083 0.15740000 0.2318333 0.07443333 0.248583062 0.003100803 FALSE FALSE
MSTRG.33214.6 ENSG00000157654 HEK293_OSMI2_2hA HEK293_TMG_2hB PALM2AKAP2 protein_coding   6.674974 4.818026 8.887592 0.3791428 0.3414699 0.8819813 0.3881936 0.3081079 0.7590809 0.01463392 0.3839947 1.2736194 0.05887083 0.06473333 0.0887000 0.02396667 0.973519836 0.003100803 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000157654 E001 0.9889485 0.0196073677 3.067324e-02 7.327934e-02 9 109498325 109498700 376 + 0.094 0.503 3.178
ENSG00000157654 E002 0.0000000       9 109514516 109514611 96 +      
ENSG00000157654 E003 0.0000000       9 109640788 109640866 79 +      
ENSG00000157654 E004 2.6488611 0.0070218890 4.786858e-05 2.709702e-04 9 109780198 109780308 111 + 0.728 0.000 -13.085
ENSG00000157654 E005 2.5078410 0.0059043303 7.592593e-05 4.087508e-04 9 109780309 109780402 94 + 0.708 0.000 -13.024
ENSG00000157654 E006 3.0354376 0.0048938042 3.331601e-03 1.123944e-02 9 109780403 109780438 36 + 0.728 0.273 -2.311
ENSG00000157654 E007 2.7430158 0.0054623533 7.790320e-03 2.325166e-02 9 109780439 109780455 17 + 0.687 0.273 -2.141
ENSG00000157654 E008 3.4073515 0.0746284993 2.144636e-01 3.438281e-01 9 109780456 109780487 32 + 0.687 0.509 -0.798
ENSG00000157654 E009 4.4308335 0.0065475592 4.701271e-02 1.038492e-01 9 109780488 109780533 46 + 0.800 0.560 -1.014
ENSG00000157654 E010 0.0000000       9 109867031 109867074 44 +      
ENSG00000157654 E011 0.0000000       9 109867271 109867490 220 +      
ENSG00000157654 E012 4.5924801 0.0291396810 1.618997e-02 4.314327e-02 9 109867491 109867571 81 + 0.847 0.504 -1.460
ENSG00000157654 E013 6.7379775 0.0028811815 2.622384e-02 6.440358e-02 9 109880551 109880681 131 + 0.953 0.731 -0.863
ENSG00000157654 E014 7.5633089 0.0022132401 4.688422e-03 1.506466e-02 9 109923735 109923849 115 + 1.018 0.731 -1.105
ENSG00000157654 E015 4.5404326 0.0035531067 7.225408e-03 2.180918e-02 9 109925061 109925082 22 + 0.848 0.504 -1.464
ENSG00000157654 E016 5.7604058 0.0027388707 4.069074e-02 9.230696e-02 9 109931927 109932028 102 + 0.904 0.694 -0.830
ENSG00000157654 E017 20.5133816 0.0009720151 2.110716e-05 1.301741e-04 9 109942686 109943381 696 + 1.413 1.137 -0.970
ENSG00000157654 E018 19.7315584 0.0169314723 6.926696e-02 1.421487e-01 9 109943382 109951476 8095 + 1.343 1.229 -0.398
ENSG00000157654 E019 8.0446195 0.0021474225 5.935125e-02 1.255294e-01 9 110015954 110016039 86 + 1.008 0.853 -0.582
ENSG00000157654 E020 1.4132927 0.0227072530 7.765014e-01 8.560729e-01 9 110048598 110048695 98 + 0.344 0.439 0.533
ENSG00000157654 E021 1.4132927 0.0227072530 7.765014e-01 8.560729e-01 9 110048696 110048697 2 + 0.344 0.439 0.533
ENSG00000157654 E022 10.1493044 0.0018922252 1.059304e-01 1.995148e-01 9 110048698 110048855 158 + 1.075 0.970 -0.385
ENSG00000157654 E023 0.0000000       9 110090197 110090416 220 +      
ENSG00000157654 E024 0.0000000       9 110125501 110125596 96 +      
ENSG00000157654 E025 1.0339774 0.0738354371 1.705911e-01 2.889351e-01 9 110127646 110128012 367 + 0.389 0.157 -1.732
ENSG00000157654 E026 86.8064450 0.0036138618 3.067576e-10 4.984269e-09 9 110136127 110136865 739 + 2.014 1.785 -0.769
ENSG00000157654 E027 165.7473693 0.0004404922 3.485248e-12 7.826810e-11 9 110136866 110138170 1305 + 2.257 2.135 -0.406
ENSG00000157654 E028 68.6334943 0.0004264625 3.429798e-06 2.535272e-05 9 110138171 110138539 369 + 1.880 1.750 -0.440
ENSG00000157654 E029 0.0000000       9 110148528 110148703 176 +      
ENSG00000157654 E030 26.9887237 0.0014745463 2.157094e-04 1.031306e-03 9 110156319 110156497 179 + 1.507 1.316 -0.661
ENSG00000157654 E031 0.0000000       9 110162094 110162132 39 +      
ENSG00000157654 E032 439.7497208 0.0048803235 1.961590e-01 3.214238e-01 9 110168399 110171119 2721 + 2.591 2.696 0.350
ENSG00000157654 E033 277.5528510 0.0043322442 3.530346e-27 4.631672e-25 9 110171120 110172512 1393 + 2.225 2.636 1.371