ENSG00000157445

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000474759 ENSG00000157445 HEK293_OSMI2_2hA HEK293_TMG_2hB CACNA2D3 protein_coding protein_coding 2.857971 3.59043 3.028416 0.1640344 0.1909253 -0.2448496 0.05711103 0.0000000 0.1653410 0.0000000 0.05702757 4.13209162 0.02008750 0.00000000 0.05443333 0.05443333 0.004118796 0.004118796 FALSE  
ENST00000477024 ENSG00000157445 HEK293_OSMI2_2hA HEK293_TMG_2hB CACNA2D3 protein_coding nonsense_mediated_decay 2.857971 3.59043 3.028416 0.1640344 0.1909253 -0.2448496 0.16752341 0.0000000 0.0000000 0.0000000 0.00000000 0.00000000 0.05348333 0.00000000 0.00000000 0.00000000   0.004118796 TRUE  
MSTRG.23097.3 ENSG00000157445 HEK293_OSMI2_2hA HEK293_TMG_2hB CACNA2D3 protein_coding   2.857971 3.59043 3.028416 0.1640344 0.1909253 -0.2448496 0.22273961 0.1725751 0.4142336 0.1725751 0.07639396 1.21636856 0.07688333 0.04670000 0.14086667 0.09416667 0.271667172 0.004118796 TRUE  
MSTRG.23097.5 ENSG00000157445 HEK293_OSMI2_2hA HEK293_TMG_2hB CACNA2D3 protein_coding   2.857971 3.59043 3.028416 0.1640344 0.1909253 -0.2448496 0.57448853 0.2979857 0.2102926 0.2979857 0.10552553 -0.48344242 0.19249167 0.08066667 0.06833333 -0.01233333 0.741912965 0.004118796 TRUE  
MSTRG.23097.7 ENSG00000157445 HEK293_OSMI2_2hA HEK293_TMG_2hB CACNA2D3 protein_coding   2.857971 3.59043 3.028416 0.1640344 0.1909253 -0.2448496 0.33961110 1.0206085 0.5216592 0.5326582 0.30277197 -0.95492273 0.12886667 0.29393333 0.18500000 -0.10893333 0.926547446 0.004118796 TRUE  
MSTRG.23097.8 ENSG00000157445 HEK293_OSMI2_2hA HEK293_TMG_2hB CACNA2D3 protein_coding   2.857971 3.59043 3.028416 0.1640344 0.1909253 -0.2448496 1.01810261 1.4908109 1.4160395 0.2899505 0.38133257 -0.07372825 0.34524167 0.40983333 0.45823333 0.04840000 0.911973775 0.004118796 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000157445 E001 0.1515154 0.0429570445 0.389117664   3 54122547 54122551 5 + 0.137 0.000 -10.062
ENSG00000157445 E002 0.5911862 0.0184814702 0.020467952 0.05247577 3 54122552 54122580 29 + 0.394 0.000 -14.437
ENSG00000157445 E003 0.5911862 0.0184814702 0.020467952 0.05247577 3 54122581 54122584 4 + 0.394 0.000 -14.437
ENSG00000157445 E004 1.1834165 0.0108694396 0.010529736 0.03004438 3 54122585 54122629 45 + 0.554 0.109 -3.172
ENSG00000157445 E005 1.1834165 0.0108694396 0.010529736 0.03004438 3 54122630 54122665 36 + 0.554 0.109 -3.172
ENSG00000157445 E006 1.1908034 0.0145074463 0.076090769 0.15325666 3 54122666 54122708 43 + 0.507 0.197 -1.952
ENSG00000157445 E007 1.3359761 0.0098896289 0.039101113 0.08937620 3 54122709 54122723 15 + 0.554 0.197 -2.173
ENSG00000157445 E008 1.4811488 0.0088457564 0.020034717 0.05156207 3 54122724 54122726 3 + 0.597 0.197 -2.366
ENSG00000157445 E009 4.0139012 0.0039638817 0.107525148 0.20188619 3 54122727 54122800 74 + 0.815 0.588 -0.949
ENSG00000157445 E010 5.5803288 0.0311035572 0.978750261 0.99080009 3 54122801 54122835 35 + 0.815 0.809 -0.023
ENSG00000157445 E011 0.0000000       3 54123461 54123478 18 +      
ENSG00000157445 E012 0.1515154 0.0429570445 0.389117664   3 54123479 54123512 34 + 0.137 0.000 -12.558
ENSG00000157445 E013 11.9640897 0.0016876205 0.802168223 0.87397912 3 54123513 54123594 82 + 1.120 1.096 -0.087
ENSG00000157445 E014 0.0000000       3 54177796 54177898 103 +      
ENSG00000157445 E015 0.0000000       3 54197371 54197507 137 +      
ENSG00000157445 E016 0.0000000       3 54272774 54273168 395 +      
ENSG00000157445 E017 18.9205621 0.0010293468 0.379152504 0.52343420 3 54320442 54320558 117 + 1.328 1.260 -0.238
ENSG00000157445 E018 17.5261816 0.0011666558 0.945778903 0.96997211 3 54386715 54386774 60 + 1.255 1.260 0.019
ENSG00000157445 E019 31.9713233 0.0006879816 0.394433114 0.53826932 3 54503492 54503654 163 + 1.479 1.531 0.177
ENSG00000157445 E020 33.5863037 0.0122362386 0.398434707 0.54218750 3 54562800 54562931 132 + 1.495 1.559 0.220
ENSG00000157445 E021 30.8136196 0.0070879813 0.924690509 0.95657169 3 54569795 54569855 61 + 1.500 1.489 -0.037
ENSG00000157445 E022 39.7345082 0.0006205507 0.332725423 0.47639579 3 54569954 54570104 151 + 1.570 1.623 0.181
ENSG00000157445 E023 30.8013864 0.0006718130 0.479026678 0.61743222 3 54581803 54581877 75 + 1.469 1.512 0.150
ENSG00000157445 E024 32.1762362 0.0007237317 0.497555287 0.63410083 3 54627787 54627876 90 + 1.490 1.531 0.141
ENSG00000157445 E025 34.6694487 0.0009341583 0.592874788 0.71533972 3 54642128 54642241 114 + 1.525 1.556 0.107
ENSG00000157445 E026 26.9225059 0.0007442908 0.496172998 0.63289779 3 54752599 54752677 79 + 1.411 1.455 0.155
ENSG00000157445 E027 31.0201138 0.0125439693 0.862126071 0.91511888 3 54764218 54764351 134 + 1.485 1.496 0.037
ENSG00000157445 E028 0.0000000       3 54816853 54816870 18 +      
ENSG00000157445 E029 22.0733243 0.0022894648 0.758523908 0.84310252 3 54837159 54837230 72 + 1.336 1.358 0.078
ENSG00000157445 E030 28.5896719 0.0007253618 0.728635446 0.82106055 3 54838568 54838648 81 + 1.446 1.468 0.076
ENSG00000157445 E031 26.9282352 0.0007877606 0.716397180 0.81207181 3 54846393 54846467 75 + 1.452 1.428 -0.082
ENSG00000157445 E032 21.3048020 0.0144791083 0.242861240 0.37754346 3 54871539 54871622 84 + 1.273 1.385 0.393
ENSG00000157445 E033 17.1621159 0.0232644066 0.171106513 0.28961390 3 54879018 54879089 72 + 1.155 1.316 0.566
ENSG00000157445 E034 2.0326021 0.0084299268 0.468675609 0.60808681 3 54879350 54879411 62 + 0.394 0.518 0.636
ENSG00000157445 E035 0.1817044 0.0395140322 0.604147313   3 54879412 54879476 65 + 0.000 0.110 11.160
ENSG00000157445 E036 3.0353438 0.0066476609 0.952656502 0.97434141 3 54880796 54880800 5 + 0.597 0.588 -0.042
ENSG00000157445 E037 5.1387955 0.0155174031 0.878424314 0.92591865 3 54880801 54880863 63 + 0.790 0.769 -0.084
ENSG00000157445 E038 4.2102249 0.0283357008 0.988741478 0.99699757 3 54885281 54885326 46 + 0.704 0.701 -0.012
ENSG00000157445 E039 5.3905314 0.0064340819 0.733491263 0.82468957 3 54885489 54885586 98 + 0.815 0.770 -0.182
ENSG00000157445 E040 7.2910736 0.0575846881 0.783255095 0.86088353 3 54887959 54888052 94 + 0.942 0.883 -0.224
ENSG00000157445 E041 5.2585112 0.0102831876 0.391097570 0.53504330 3 54891355 54891450 96 + 0.862 0.747 -0.454
ENSG00000157445 E042 6.7344565 0.0025545285 0.633683181 0.74833021 3 54896749 54896870 122 + 0.839 0.897 0.222
ENSG00000157445 E043 5.2316516 0.0033850856 0.125375564 0.22767857 3 54899788 54899868 81 + 0.636 0.847 0.860
ENSG00000157445 E044 0.9190928 0.2554073447 0.829844519 0.89318150 3 54912294 54912387 94 + 0.240 0.276 0.269
ENSG00000157445 E045 2.8721896 0.0054156037 0.320565503 0.46371337 3 54968450 54968511 62 + 0.671 0.518 -0.686
ENSG00000157445 E046 2.5744573 0.0065039621 0.323182138 0.46650922 3 54969800 54969844 45 + 0.636 0.478 -0.726
ENSG00000157445 E047 3.7653730 0.0042787319 0.225283902 0.35675538 3 54984608 54984670 63 + 0.763 0.588 -0.742
ENSG00000157445 E048 0.1482932 0.0411267835 0.606752325   3 54987678 54987682 5 + 0.000 0.109 11.155
ENSG00000157445 E049 3.4193922 0.0672804650 0.743724775 0.83229258 3 54987683 54987753 71 + 0.671 0.618 -0.228
ENSG00000157445 E050 4.9965982 0.0064779551 0.005630915 0.01763298 3 55004763 55004838 76 + 0.960 0.587 -1.501
ENSG00000157445 E051 0.0000000       3 55007776 55007789 14 +      
ENSG00000157445 E052 4.8232973 0.0035104178 0.078076023 0.15638401 3 55007790 55007842 53 + 0.883 0.648 -0.949
ENSG00000157445 E053 5.8949617 0.0030810078 0.457306209 0.59764962 3 55009388 55009443 56 + 0.883 0.790 -0.364
ENSG00000157445 E054 6.8965746 0.0025322410 0.020115847 0.05173126 3 55018206 55018317 112 + 1.040 0.769 -1.032
ENSG00000157445 E055 5.8938038 0.0028623246 0.283031094 0.42310278 3 55073445 55073557 113 + 0.904 0.769 -0.524
ENSG00000157445 E056 0.0000000       3 55073558 55073559 2 +      
ENSG00000157445 E057 0.0000000       3 55073560 55073575 16 +      
ENSG00000157445 E058 0.6934950 0.0197759852 0.809091967 0.87873307 3 55073576 55073776 201 + 0.240 0.197 -0.361
ENSG00000157445 E059 2.7219193 0.0347505540 0.672007971 0.77810649 3 55073777 55073778 2 + 0.597 0.520 -0.354
ENSG00000157445 E060 6.5226770 0.0028000686 0.983021119 0.99345664 3 55073779 55073859 81 + 0.862 0.864 0.008
ENSG00000157445 E061 10.6060399 0.0017274468 0.176990076 0.29732770 3 55074114 55074557 444 + 0.977 1.115 0.503