ENSG00000157404

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000288135 ENSG00000157404 HEK293_OSMI2_2hA HEK293_TMG_2hB KIT protein_coding protein_coding 2.495552 0.768081 3.547376 0.08478354 0.2075693 2.192821 0.558837640 0.04676587 1.14516106 0.04676587 0.59656894 4.3469265 0.155766667 0.06336667 0.3247667 0.26140000 0.6026429 0.0415541 FALSE TRUE
ENST00000685816 ENSG00000157404 HEK293_OSMI2_2hA HEK293_TMG_2hB KIT protein_coding protein_coding 2.495552 0.768081 3.547376 0.08478354 0.2075693 2.192821 0.005159793 0.04127834 0.00000000 0.04127834 0.00000000 -2.3583497 0.008079167 0.06463333 0.0000000 -0.06463333 0.4617897 0.0415541 FALSE FALSE
ENST00000686011 ENSG00000157404 HEK293_OSMI2_2hA HEK293_TMG_2hB KIT protein_coding protein_coding 2.495552 0.768081 3.547376 0.08478354 0.2075693 2.192821 0.612849832 0.00000000 0.60434889 0.00000000 0.33429840 5.9409863 0.183083333 0.00000000 0.1695667 0.16956667 0.4337122 0.0415541 FALSE TRUE
ENST00000687246 ENSG00000157404 HEK293_OSMI2_2hA HEK293_TMG_2hB KIT protein_coding protein_coding 2.495552 0.768081 3.547376 0.08478354 0.2075693 2.192821 0.040581799 0.06135402 0.00000000 0.06135402 0.00000000 -2.8349947 0.082629167 0.06613333 0.0000000 -0.06613333 0.3544555 0.0415541 FALSE TRUE
ENST00000687295 ENSG00000157404 HEK293_OSMI2_2hA HEK293_TMG_2hB KIT protein_coding protein_coding 2.495552 0.768081 3.547376 0.08478354 0.2075693 2.192821 0.585237371 0.00000000 0.90048220 0.00000000 0.90048220 6.5085589 0.161462500 0.00000000 0.2475000 0.24750000 1.0000000 0.0415541 FALSE TRUE
ENST00000688060 ENSG00000157404 HEK293_OSMI2_2hA HEK293_TMG_2hB KIT protein_coding processed_transcript 2.495552 0.768081 3.547376 0.08478354 0.2075693 2.192821 0.195792451 0.28044404 0.00000000 0.17774659 0.00000000 -4.8601883 0.184445833 0.32370000 0.0000000 -0.32370000 0.0415541 0.0415541 FALSE TRUE
ENST00000689994 ENSG00000157404 HEK293_OSMI2_2hA HEK293_TMG_2hB KIT protein_coding protein_coding 2.495552 0.768081 3.547376 0.08478354 0.2075693 2.192821 0.011621681 0.09297345 0.00000000 0.09297345 0.00000000 -3.3642005 0.018200000 0.14560000 0.0000000 -0.14560000 0.5490505 0.0415541 FALSE TRUE
ENST00000690543 ENSG00000157404 HEK293_OSMI2_2hA HEK293_TMG_2hB KIT protein_coding protein_coding 2.495552 0.768081 3.547376 0.08478354 0.2075693 2.192821 0.256139516 0.02464382 0.33508548 0.02464382 0.33508548 3.3162838 0.072854167 0.03340000 0.1063333 0.07293333 0.8851272 0.0415541 FALSE TRUE
ENST00000691361 ENSG00000157404 HEK293_OSMI2_2hA HEK293_TMG_2hB KIT protein_coding processed_transcript 2.495552 0.768081 3.547376 0.08478354 0.2075693 2.192821 0.027509676 0.09742788 0.00000000 0.09742788 0.00000000 -3.4252966 0.034487500 0.13203333 0.0000000 -0.13203333 0.5232727 0.0415541 FALSE TRUE
ENST00000692991 ENSG00000157404 HEK293_OSMI2_2hA HEK293_TMG_2hB KIT protein_coding processed_transcript 2.495552 0.768081 3.547376 0.08478354 0.2075693 2.192821 0.014330163 0.07861896 0.03602235 0.07861896 0.03602235 -0.9452809 0.016625000 0.12310000 0.0099000 -0.11320000 0.7590530 0.0415541 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000157404 E001 0.0000000       4 54657267 54657355 89 +      
ENSG00000157404 E002 0.0000000       4 54657889 54657912 24 +      
ENSG00000157404 E003 0.0000000       4 54657913 54657922 10 +      
ENSG00000157404 E004 0.5870335 0.0847591516 7.876374e-01 8.639597e-01 4 54657923 54657938 16 + 0.173 0.000 -10.739
ENSG00000157404 E005 0.5870335 0.0847591516 7.876374e-01 8.639597e-01 4 54657939 54657941 3 + 0.173 0.000 -12.729
ENSG00000157404 E006 0.5870335 0.0847591516 7.876374e-01 8.639597e-01 4 54657942 54657946 5 + 0.173 0.000 -12.729
ENSG00000157404 E007 1.0225516 0.4060050757 6.078600e-01 7.277566e-01 4 54657947 54657956 10 + 0.267 0.000 -13.031
ENSG00000157404 E008 1.1698006 0.1635078866 3.594107e-01 5.037456e-01 4 54657957 54657966 10 + 0.296 0.000 -13.431
ENSG00000157404 E009 4.6333814 0.0462314775 5.693294e-01 6.959109e-01 4 54657967 54658014 48 + 0.645 0.555 -0.400
ENSG00000157404 E010 6.4345008 0.0025852905 1.681606e-01 2.858048e-01 4 54658015 54658081 67 + 0.770 0.556 -0.914
ENSG00000157404 E011 0.4720498 0.1776864537 3.576563e-01 5.020742e-01 4 54658114 54658289 176 + 0.095 0.271 1.829
ENSG00000157404 E012 0.1515154 0.0428991450 8.735151e-01   4 54658563 54658707 145 + 0.050 0.000 -11.036
ENSG00000157404 E013 0.0000000       4 54658748 54658944 197 +      
ENSG00000157404 E014 0.0000000       4 54658945 54659229 285 +      
ENSG00000157404 E015 0.0000000       4 54665099 54665335 237 +      
ENSG00000157404 E016 16.1143711 0.0011312767 4.433843e-02 9.900500e-02 4 54695512 54695781 270 + 1.118 0.899 -0.806
ENSG00000157404 E017 0.0000000       4 54695782 54695906 125 +      
ENSG00000157404 E018 22.9938899 0.0178557193 2.296400e-01 3.620075e-01 4 54698284 54698565 282 + 1.250 1.146 -0.367
ENSG00000157404 E019 19.2205321 0.0078513870 9.840580e-02 1.880884e-01 4 54699630 54699766 137 + 1.185 1.022 -0.588
ENSG00000157404 E020 1.1093195 0.0115650773 1.000000e+00 1.000000e+00 4 54703721 54703723 3 + 0.239 0.271 0.239
ENSG00000157404 E021 18.6846532 0.0009963825 5.166210e-02 1.121154e-01 4 54703724 54703892 169 + 1.175 0.985 -0.688
ENSG00000157404 E022 16.1370489 0.0011613859 1.827390e-03 6.688049e-03 4 54707098 54707287 190 + 1.134 0.727 -1.542
ENSG00000157404 E023 12.5565207 0.0014986854 2.295188e-02 5.764553e-02 4 54709424 54709539 116 + 1.028 0.727 -1.159
ENSG00000157404 E024 0.1451727 0.0429217748 8.718877e-01   4 54716663 54716815 153 + 0.050 0.000 -11.035
ENSG00000157404 E025 0.1515154 0.0428991450 8.735151e-01   4 54723033 54723104 72 + 0.050 0.000 -11.036
ENSG00000157404 E026 13.0914155 0.0014320190 5.556769e-01 6.845134e-01 4 54723584 54723698 115 + 1.018 0.985 -0.122
ENSG00000157404 E027 16.3911701 0.0011597709 4.947373e-01 6.316524e-01 4 54725857 54726038 182 + 1.080 1.196 0.418
ENSG00000157404 E028 3.5996926 0.0395842660 3.112368e-01 4.536833e-01 4 54726039 54726050 12 + 0.506 0.727 0.975
ENSG00000157404 E029 12.0603271 0.0016746809 2.796882e-01 4.194493e-01 4 54727218 54727324 107 + 0.946 1.118 0.630
ENSG00000157404 E030 13.5075922 0.0182150736 2.213509e-01 3.520485e-01 4 54727416 54727542 127 + 1.038 0.899 -0.517
ENSG00000157404 E031 12.5419207 0.0014710586 2.672723e-01 4.055679e-01 4 54727823 54727927 105 + 1.008 0.899 -0.406
ENSG00000157404 E032 11.7670531 0.0015293706 3.930653e-01 5.369443e-01 4 54728011 54728121 111 + 0.975 0.899 -0.285
ENSG00000157404 E033 0.2966881 0.0274424043 1.000000e+00   4 54729292 54729334 43 + 0.095 0.000 -11.984
ENSG00000157404 E034 10.8060267 0.0016069826 5.555375e-01 6.843773e-01 4 54729335 54729400 66 + 0.940 0.899 -0.154
ENSG00000157404 E035 11.8099275 0.0015543034 6.171227e-01 7.350046e-01 4 54729401 54729485 85 + 0.969 0.944 -0.094
ENSG00000157404 E036 4.1374839 0.0040036623 3.125612e-01 4.550912e-01 4 54731328 54731330 3 + 0.608 0.437 -0.820
ENSG00000157404 E037 11.9424147 0.0014697019 5.867606e-01 7.102527e-01 4 54731331 54731419 89 + 0.975 0.944 -0.115
ENSG00000157404 E038 0.0000000       4 54731763 54731792 30 +      
ENSG00000157404 E039 13.8526236 0.0013508828 9.420064e-01 9.675661e-01 4 54731871 54731998 128 + 1.023 1.057 0.123
ENSG00000157404 E040 0.0000000       4 54731999 54733069 1071 +      
ENSG00000157404 E041 11.2158327 0.0114803525 4.865203e-01 6.242423e-01 4 54733070 54733192 123 + 0.958 0.899 -0.220
ENSG00000157404 E042 0.8889184 0.0134817506 8.318396e-01 8.946122e-01 4 54733193 54735122 1930 + 0.207 0.271 0.500
ENSG00000157404 E043 0.8846520 0.0136968632 8.328914e-01 8.953114e-01 4 54735322 54736497 1176 + 0.207 0.271 0.500
ENSG00000157404 E044 11.7705268 0.0018124884 3.452301e-01 4.893149e-01 4 54736498 54736609 112 + 0.986 0.899 -0.327
ENSG00000157404 E045 12.4444420 0.0014324110 4.462796e-01 5.877788e-01 4 54736721 54736820 100 + 1.002 0.944 -0.217
ENSG00000157404 E046 12.3741203 0.0159289722 3.067377e-01 4.489214e-01 4 54737175 54737280 106 + 1.007 0.898 -0.409
ENSG00000157404 E047 0.1482932 0.0415348721 5.631455e-02   4 54737281 54737592 312 + 0.000 0.270 13.893
ENSG00000157404 E048 125.7845726 0.0088089805 1.176085e-08 1.431362e-07 4 54738429 54740783 2355 + 1.896 2.193 0.996