ENSG00000157216

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000371319 ENSG00000157216 HEK293_OSMI2_2hA HEK293_TMG_2hB SSBP3 protein_coding protein_coding 36.6012 52.24908 33.1197 2.527866 0.8497583 -0.6575566 10.006938 16.811740 9.621658 2.2239951 0.9831710 -0.8044708 0.2564083 0.32150000 0.2913000 -0.03020000 8.525820e-01 3.333272e-07 FALSE TRUE
ENST00000417664 ENSG00000157216 HEK293_OSMI2_2hA HEK293_TMG_2hB SSBP3 protein_coding protein_coding 36.6012 52.24908 33.1197 2.527866 0.8497583 -0.6575566 16.525701 27.582743 9.233968 1.8712276 0.4850232 -1.5777047 0.4494167 0.53230000 0.2799000 -0.25240000 3.321303e-03 3.333272e-07 FALSE TRUE
ENST00000426150 ENSG00000157216 HEK293_OSMI2_2hA HEK293_TMG_2hB SSBP3 protein_coding processed_transcript 36.6012 52.24908 33.1197 2.527866 0.8497583 -0.6575566 2.986051 2.068584 4.240789 2.0685838 0.5542405 1.0321298 0.0818500 0.03623333 0.1273667 0.09113333 1.115294e-01 3.333272e-07 FALSE FALSE
MSTRG.1247.10 ENSG00000157216 HEK293_OSMI2_2hA HEK293_TMG_2hB SSBP3 protein_coding   36.6012 52.24908 33.1197 2.527866 0.8497583 -0.6575566 4.456057 2.630768 6.857551 0.5427829 0.6458824 1.3788384 0.1401792 0.05060000 0.2068333 0.15623333 3.333272e-07 3.333272e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000157216 E001 1246.9726080 0.0017313877 7.641634e-15 2.560008e-13 1 54225433 54226717 1285 - 2.936 3.098 0.540
ENSG00000157216 E002 386.0598337 0.0005335557 8.287270e-12 1.748328e-10 1 54226718 54227016 299 - 2.430 2.587 0.525
ENSG00000157216 E003 134.0795679 0.0076431436 2.668376e-01 4.051024e-01 1 54227017 54227054 38 - 2.044 2.111 0.224
ENSG00000157216 E004 342.7613111 0.0063332923 7.994591e-01 8.720757e-01 1 54227055 54227160 106 - 2.500 2.496 -0.016
ENSG00000157216 E005 397.8259192 0.0032966278 2.469601e-01 3.823653e-01 1 54228255 54228356 102 - 2.585 2.552 -0.110
ENSG00000157216 E006 261.1406980 0.0004042139 1.316895e-01 2.364914e-01 1 54228449 54228477 29 - 2.403 2.369 -0.114
ENSG00000157216 E007 314.0305864 0.0001649414 6.365733e-01 7.505589e-01 1 54228748 54228826 79 - 2.463 2.456 -0.025
ENSG00000157216 E008 7.4780860 0.0051845371 6.917724e-01 7.931790e-01 1 54228827 54229455 629 - 0.921 0.874 -0.179
ENSG00000157216 E009 1.5447358 0.0108264716 2.029669e-02 5.210367e-02 1 54238989 54239095 107 - 0.624 0.229 -2.210
ENSG00000157216 E010 0.8889184 0.0136125448 1.267680e-02 3.515041e-02 1 54239096 54239128 33 - 0.517 0.090 -3.310
ENSG00000157216 E011 353.6300785 0.0002661773 4.182010e-01 5.614869e-01 1 54239129 54239199 71 - 2.517 2.503 -0.048
ENSG00000157216 E012 282.3750072 0.0009268871 5.604904e-01 6.884898e-01 1 54240905 54240959 55 - 2.418 2.405 -0.042
ENSG00000157216 E013 259.0571639 0.0001947494 3.859856e-01 5.301852e-01 1 54241474 54241509 36 - 2.385 2.366 -0.061
ENSG00000157216 E014 252.6129765 0.0001989802 4.177493e-01 5.610674e-01 1 54242164 54242212 49 - 2.374 2.357 -0.057
ENSG00000157216 E015 2.3679618 0.0810836716 4.387544e-02 9.815629e-02 1 54242213 54242855 643 - 0.710 0.338 -1.812
ENSG00000157216 E016 2.1475462 0.4057766569 4.363344e-01 5.784337e-01 1 54242856 54243188 333 - 0.575 0.396 -0.887
ENSG00000157216 E017 1.0383430 0.0255176821 1.057629e-01 1.992567e-01 1 54243189 54243234 46 - 0.452 0.166 -1.982
ENSG00000157216 E018 167.7230311 0.0002576407 9.603806e-01 9.790366e-01 1 54243235 54243236 2 - 2.182 2.186 0.013
ENSG00000157216 E019 251.0628755 0.0002273075 6.496892e-01 7.608671e-01 1 54243237 54243271 35 - 2.365 2.357 -0.029
ENSG00000157216 E020 229.9402436 0.0001903381 5.361047e-01 6.678597e-01 1 54243272 54243299 28 - 2.331 2.317 -0.044
ENSG00000157216 E021 3.2125928 0.0331843846 8.015219e-01 8.735311e-01 1 54243300 54243323 24 - 0.574 0.624 0.220
ENSG00000157216 E022 287.6420970 0.0009916812 4.969394e-02 1.086144e-01 1 54251616 54251692 77 - 2.451 2.402 -0.163
ENSG00000157216 E023 252.1245779 0.0002423537 6.180095e-02 1.296554e-01 1 54251794 54251860 67 - 2.390 2.347 -0.143
ENSG00000157216 E024 1.1479526 0.4532188807 8.012161e-01 8.733335e-01 1 54255597 54255685 89 - 0.377 0.283 -0.594
ENSG00000157216 E025 207.7873175 0.0017543216 4.778978e-01 6.163797e-01 1 54257127 54257186 60 - 2.291 2.270 -0.069
ENSG00000157216 E026 1.7724074 0.4390113264 8.055428e-01 8.763656e-01 1 54257187 54257266 80 - 0.375 0.425 0.277
ENSG00000157216 E027 89.9453856 0.0003118447 7.469350e-01 8.345843e-01 1 54258069 54258149 81 - 1.924 1.913 -0.035
ENSG00000157216 E028 222.6279488 0.0003060868 7.568095e-01 8.418539e-01 1 54281438 54281527 90 - 2.300 2.310 0.035
ENSG00000157216 E029 0.0000000       1 54325369 54325439 71 -      
ENSG00000157216 E030 0.3666179 0.0303244232 6.172782e-01 7.351253e-01 1 54326326 54326472 147 - 0.164 0.090 -0.984
ENSG00000157216 E031 0.0000000       1 54335797 54335881 85 -      
ENSG00000157216 E032 0.0000000       1 54356415 54356818 404 -      
ENSG00000157216 E033 251.7028299 0.0043536859 4.550160e-02 1.011129e-01 1 54401861 54401945 85 - 2.412 2.338 -0.246
ENSG00000157216 E034 0.0000000       1 54401946 54402409 464 -      
ENSG00000157216 E035 230.1736064 0.0030884843 7.798276e-04 3.186850e-03 1 54404576 54404637 62 - 2.400 2.284 -0.388
ENSG00000157216 E036 238.8117943 0.0064969521 6.439352e-04 2.694101e-03 1 54404858 54404930 73 - 2.437 2.289 -0.494
ENSG00000157216 E037 0.2903454 0.3360313901 1.239044e-01   1 54405449 54405504 56 - 0.282 0.000 -10.451
ENSG00000157216 E038 218.1644400 0.0212811995 1.774625e-04 8.673821e-04 1 54405953 54406499 547 - 2.469 2.203 -0.890
ENSG00000157216 E039 0.0000000       1 54412708 54412877 170 -      
ENSG00000157216 E040 0.0000000       1 54413081 54413253 173 -      
ENSG00000157216 E041 0.0000000       1 54413356 54413479 124 -