ENSG00000157014

Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000426850 ENSG00000157014 HEK293_OSMI2_2hA HEK293_TMG_2hB TATDN2 protein_coding protein_coding 61.99976 95.04063 40.8511 4.167044 0.7330946 -1.217968 8.891559 13.72612 5.613788 1.571388 1.4151668 -1.2883608 0.1442917 0.1437333 0.13626667 -0.007466667 9.174108e-01 6.114385e-08 FALSE TRUE
ENST00000448281 ENSG00000157014 HEK293_OSMI2_2hA HEK293_TMG_2hB TATDN2 protein_coding protein_coding 61.99976 95.04063 40.8511 4.167044 0.7330946 -1.217968 38.180014 50.24109 31.596777 4.435067 0.2015816 -0.6689209 0.6461167 0.5279333 0.77413333 0.246200000 7.706410e-06 6.114385e-08 FALSE TRUE
ENST00000496355 ENSG00000157014 HEK293_OSMI2_2hA HEK293_TMG_2hB TATDN2 protein_coding processed_transcript 61.99976 95.04063 40.8511 4.167044 0.7330946 -1.217968 14.721589 30.68355 3.547949 2.365466 0.5052863 -3.1088177 0.2058708 0.3242000 0.08736667 -0.236833333 6.114385e-08 6.114385e-08 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.