ENSG00000156976

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000323963 ENSG00000156976 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF4A2 protein_coding protein_coding 99.25201 46.46757 128.7868 4.266382 2.568693 1.47049 24.766467 13.310474 25.082346 1.11854577 1.7620597 0.9136019 0.25995417 0.28706667 0.1945000000 -0.092566667 5.338261e-04 8.432263e-08 FALSE TRUE
ENST00000363450 ENSG00000156976 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF4A2 protein_coding snoRNA 99.25201 46.46757 128.7868 4.266382 2.568693 1.47049 2.946017 3.254838 0.000000 3.25483773 0.0000000 -8.3508675 0.02050417 0.06653333 0.0000000000 -0.066533333 3.690497e-01 8.432263e-08   FALSE
ENST00000485101 ENSG00000156976 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF4A2 protein_coding retained_intron 99.25201 46.46757 128.7868 4.266382 2.568693 1.47049 6.777684 1.614483 10.534683 0.05251937 0.6263287 2.6984636 0.06282500 0.03553333 0.0818000000 0.046266667 6.904612e-06 8.432263e-08   FALSE
ENST00000496382 ENSG00000156976 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF4A2 protein_coding retained_intron 99.25201 46.46757 128.7868 4.266382 2.568693 1.47049 1.661896 4.676989 0.080057 4.67698856 0.0800570 -5.7016790 0.03467083 0.08940000 0.0006333333 -0.088766667 8.714077e-01 8.432263e-08   FALSE
ENST00000497177 ENSG00000156976 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF4A2 protein_coding retained_intron 99.25201 46.46757 128.7868 4.266382 2.568693 1.47049 12.382742 4.681314 16.768233 0.43782808 0.6357216 1.8385268 0.12561667 0.10196667 0.1304000000 0.028433333 3.031911e-01 8.432263e-08   FALSE
MSTRG.24239.18 ENSG00000156976 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF4A2 protein_coding   99.25201 46.46757 128.7868 4.266382 2.568693 1.47049 7.942358 2.992748 13.817259 1.54759670 1.0245548 2.2031598 0.07709167 0.05950000 0.1072000000 0.047700000 6.873152e-01 8.432263e-08 TRUE TRUE
MSTRG.24239.7 ENSG00000156976 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF4A2 protein_coding   99.25201 46.46757 128.7868 4.266382 2.568693 1.47049 13.411300 7.089472 20.929045 0.25468106 3.7221272 1.5604119 0.13066250 0.15580000 0.1615333333 0.005733333 9.843051e-01 8.432263e-08 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000156976 E001 0.3332198 0.0295048182 4.262511e-01   3 186783205 186783304 100 + 0.073 0.199 1.675
ENSG00000156976 E002 0.3332198 0.0295048182 4.262511e-01   3 186783305 186783350 46 + 0.073 0.199 1.675
ENSG00000156976 E003 0.7708142 0.0153769730 7.958236e-01 8.696247e-01 3 186783351 186783447 97 + 0.238 0.199 -0.325
ENSG00000156976 E004 1.3253550 0.1380688447 7.444959e-02 1.506526e-01 3 186783448 186783571 124 + 0.421 0.000 -12.836
ENSG00000156976 E005 0.8877605 0.4240338485 3.029115e-01 4.448260e-01 3 186783572 186783573 2 + 0.321 0.000 -12.254
ENSG00000156976 E006 0.8877605 0.4240338485 3.029115e-01 4.448260e-01 3 186783574 186783574 1 + 0.321 0.000 -12.254
ENSG00000156976 E007 0.8877605 0.4240338485 3.029115e-01 4.448260e-01 3 186783575 186783576 2 + 0.321 0.000 -12.254
ENSG00000156976 E008 0.8856842 0.0778453578 1.450125e-01 2.548511e-01 3 186783577 186783577 1 + 0.321 0.000 -12.253
ENSG00000156976 E009 0.8856842 0.0778453578 1.450125e-01 2.548511e-01 3 186783578 186783585 8 + 0.321 0.000 -12.253
ENSG00000156976 E010 77.6313985 0.0004480576 4.723692e-12 1.036832e-10 3 186783586 186783592 7 + 1.916 1.589 -1.107
ENSG00000156976 E011 104.5583843 0.0017913321 1.240592e-12 2.998551e-11 3 186783593 186783596 4 + 2.042 1.727 -1.063
ENSG00000156976 E012 177.9617347 0.0003055672 6.486526e-09 8.286264e-08 3 186783597 186783639 43 + 2.242 2.088 -0.516
ENSG00000156976 E013 19.4849700 0.0009057408 3.344701e-11 6.390113e-10 3 186784203 186784428 226 + 1.379 0.592 -2.980
ENSG00000156976 E014 60.9787564 0.0008138684 4.118330e-06 2.985631e-05 3 186784429 186784431 3 + 1.796 1.569 -0.772
ENSG00000156976 E015 235.0707093 0.0002249225 6.098271e-06 4.257755e-05 3 186784432 186784477 46 + 2.348 2.258 -0.301
ENSG00000156976 E016 39.7033531 0.0005539448 3.611776e-09 4.826410e-08 3 186784478 186784563 86 + 1.641 1.238 -1.394
ENSG00000156976 E017 371.7418475 0.0013315842 2.433678e-03 8.563072e-03 3 186784564 186784696 133 + 2.535 2.484 -0.171
ENSG00000156976 E018 41.0166986 0.0005341425 7.195124e-15 2.423279e-13 3 186784697 186784795 99 + 1.673 1.081 -2.060
ENSG00000156976 E019 49.9863022 0.0421524221 6.847545e-05 3.727614e-04 3 186784796 186784961 166 + 1.743 1.277 -1.598
ENSG00000156976 E020 264.7017934 0.0002106312 2.422258e-02 6.027040e-02 3 186784962 186785060 99 + 2.349 2.437 0.296
ENSG00000156976 E021 230.8395082 0.0006147749 2.429925e-01 3.777072e-01 3 186785061 186785101 41 + 2.296 2.362 0.219
ENSG00000156976 E022 97.9241689 0.0139192292 1.300092e-09 1.887201e-08 3 186785102 186785882 781 + 2.029 1.580 -1.517
ENSG00000156976 E023 375.0841686 0.0001491773 2.094412e-01 3.378691e-01 3 186785883 186785961 79 + 2.524 2.532 0.025
ENSG00000156976 E024 280.3235033 0.0001732189 2.241003e-01 3.553176e-01 3 186785962 186785994 33 + 2.399 2.404 0.015
ENSG00000156976 E025 346.2869806 0.0001539425 2.793136e-01 4.190436e-01 3 186785995 186786051 57 + 2.489 2.499 0.035
ENSG00000156976 E026 5.7821386 0.0104796969 1.753361e-01 2.951118e-01 3 186786052 186786075 24 + 0.834 0.652 -0.740
ENSG00000156976 E027 13.7669400 0.0115054049 7.688568e-04 3.146157e-03 3 186786076 186786163 88 + 1.202 0.795 -1.513
ENSG00000156976 E028 411.9189449 0.0001910349 1.930291e-01 3.175697e-01 3 186786164 186786273 110 + 2.549 2.609 0.199
ENSG00000156976 E029 17.8889592 0.0010276874 3.546336e-06 2.612040e-05 3 186786274 186786322 49 + 1.315 0.834 -1.758
ENSG00000156976 E030 34.3517918 0.0055617526 5.449250e-07 4.784909e-06 3 186786323 186786490 168 + 1.582 1.174 -1.417
ENSG00000156976 E031 14.3720186 0.0033075391 5.457198e-02 1.172414e-01 3 186786491 186786501 11 + 1.187 1.013 -0.631
ENSG00000156976 E032 463.7215471 0.0001389112 7.143282e-04 2.950043e-03 3 186786502 186786639 138 + 2.591 2.686 0.319
ENSG00000156976 E033 215.0433303 0.0001648366 2.787965e-02 6.777599e-02 3 186786640 186786645 6 + 2.259 2.352 0.309
ENSG00000156976 E034 14.3272673 0.0042138928 4.223670e-02 9.515426e-02 3 186786646 186786671 26 + 1.182 0.988 -0.703
ENSG00000156976 E035 12.1451495 0.0035212181 1.401591e-02 3.826268e-02 3 186786672 186786674 3 + 1.133 0.869 -0.975
ENSG00000156976 E036 68.1915204 0.0152275723 1.097919e-04 5.670131e-04 3 186786675 186787126 452 + 1.853 1.564 -0.982
ENSG00000156976 E037 499.7607987 0.0009578307 1.619505e-03 6.023566e-03 3 186787127 186787264 138 + 2.621 2.723 0.341
ENSG00000156976 E038 25.5208641 0.0007475393 2.622135e-04 1.225619e-03 3 186787265 186787299 35 + 1.438 1.158 -0.981
ENSG00000156976 E039 19.3407738 0.0009356318 5.578759e-05 3.105819e-04 3 186787300 186787313 14 + 1.338 0.961 -1.351
ENSG00000156976 E040 40.0750639 0.0037534760 1.351024e-01 2.412313e-01 3 186787314 186787406 93 + 1.584 1.511 -0.250
ENSG00000156976 E041 44.5931108 0.0006755749 2.142539e-02 5.448509e-02 3 186787407 186787478 72 + 1.636 1.526 -0.375
ENSG00000156976 E042 29.3108804 0.0025886919 3.647684e-03 1.214850e-02 3 186787479 186787494 16 + 1.482 1.279 -0.702
ENSG00000156976 E043 396.6636659 0.0002472427 8.444106e-08 8.695496e-07 3 186787495 186787584 90 + 2.508 2.649 0.470
ENSG00000156976 E044 22.1069654 0.0011180942 4.108344e-03 1.344632e-02 3 186787585 186787611 27 + 1.366 1.140 -0.796
ENSG00000156976 E045 59.5415507 0.0194035153 3.332878e-05 1.959482e-04 3 186787612 186787775 164 + 1.806 1.445 -1.230
ENSG00000156976 E046 34.0368642 0.0088779150 9.418238e-06 6.297896e-05 3 186787776 186787802 27 + 1.571 1.192 -1.318
ENSG00000156976 E047 417.9195399 0.0015142567 2.167438e-04 1.035703e-03 3 186787803 186787882 80 + 2.534 2.661 0.423
ENSG00000156976 E048 210.1204129 0.0001742184 1.685287e-10 2.862147e-09 3 186787883 186788309 427 + 2.313 2.159 -0.516
ENSG00000156976 E049 334.2018831 0.0006734362 1.533317e-01 2.662093e-01 3 186788310 186788416 107 + 2.478 2.477 -0.004
ENSG00000156976 E050 151.9792487 0.0002370943 1.020119e-01 1.935798e-01 3 186788417 186788420 4 + 2.143 2.124 -0.065
ENSG00000156976 E051 528.3518961 0.0033123655 1.065090e-02 3.033529e-02 3 186788421 186789124 704 + 2.687 2.643 -0.150
ENSG00000156976 E052 1193.8592099 0.0063504867 8.480048e-10 1.273096e-08 3 186789125 186789897 773 + 2.944 3.196 0.837