ENSG00000156858

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000300835 ENSG00000156858 HEK293_OSMI2_2hA HEK293_TMG_2hB PRR14 protein_coding protein_coding 62.33883 85.96988 47.55904 5.072315 0.8781029 -0.8539763 24.667494 43.576569 14.801978 1.2434504 0.9425564 -1.55711937 0.37547083 0.50993333 0.31140000 -0.198533333 0.0003500491 2.190897e-13 FALSE TRUE
ENST00000542965 ENSG00000156858 HEK293_OSMI2_2hA HEK293_TMG_2hB PRR14 protein_coding protein_coding 62.33883 85.96988 47.55904 5.072315 0.8781029 -0.8539763 13.558553 18.727422 10.417916 1.8449224 0.4813463 -0.84547164 0.21592083 0.21710000 0.21883333 0.001733333 0.9918211887 2.190897e-13 FALSE TRUE
ENST00000567989 ENSG00000156858 HEK293_OSMI2_2hA HEK293_TMG_2hB PRR14 protein_coding retained_intron 62.33883 85.96988 47.55904 5.072315 0.8781029 -0.8539763 5.209531 4.610407 4.535716 0.8859914 0.3371990 -0.02351252 0.09147917 0.05323333 0.09543333 0.042200000 0.0362366918 2.190897e-13 FALSE FALSE
MSTRG.12477.11 ENSG00000156858 HEK293_OSMI2_2hA HEK293_TMG_2hB PRR14 protein_coding   62.33883 85.96988 47.55904 5.072315 0.8781029 -0.8539763 5.793368 7.208667 4.425175 1.0513104 0.9927055 -0.70274124 0.09281667 0.08436667 0.09330000 0.008933333 0.9400719851 2.190897e-13 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000156858 E001 4.105035 0.0566519743 1.104170e-01 2.061493e-01 16 30650325 30650603 279 + 0.835 0.563 -1.139
ENSG00000156858 E002 2.393912 0.0060881452 5.001343e-03 1.593357e-02 16 30650604 30650676 73 + 0.768 0.320 -2.157
ENSG00000156858 E003 2.097224 0.0076132861 2.508966e-02 6.205981e-02 16 30650677 30650716 40 + 0.689 0.320 -1.835
ENSG00000156858 E004 1.209339 0.0800347743 2.087706e-02 5.332835e-02 16 30650717 30650728 12 + 0.592 0.157 -2.745
ENSG00000156858 E005 5.776740 0.0057205428 7.705109e-01 8.518083e-01 16 30650729 30650777 49 + 0.802 0.764 -0.154
ENSG00000156858 E006 4.897398 0.0039215926 1.500745e-01 2.617768e-01 16 30650778 30650779 2 + 0.535 0.764 0.982
ENSG00000156858 E007 28.442736 0.0262192467 1.141547e-02 3.215132e-02 16 30650780 30650806 27 + 1.579 1.329 -0.859
ENSG00000156858 E008 10.791032 0.0252872652 1.623501e-02 4.324260e-02 16 30650878 30650935 58 + 1.219 0.925 -1.070
ENSG00000156858 E009 36.211782 0.0352961260 2.769545e-01 4.164539e-01 16 30650936 30650959 24 + 1.611 1.483 -0.439
ENSG00000156858 E010 77.825786 0.0183799349 3.849738e-01 5.291226e-01 16 30650960 30650984 25 + 1.896 1.825 -0.240
ENSG00000156858 E011 81.251414 0.0116871959 5.097338e-01 6.450264e-01 16 30650985 30650992 8 + 1.896 1.851 -0.151
ENSG00000156858 E012 269.892320 0.0049625637 1.283626e-01 2.318708e-01 16 30650993 30651127 135 + 2.421 2.364 -0.191
ENSG00000156858 E013 269.290197 0.0049061629 1.926260e-02 4.990347e-02 16 30651190 30651595 406 + 2.443 2.352 -0.303
ENSG00000156858 E014 148.481400 0.0027159272 3.169033e-01 4.597142e-01 16 30651596 30651668 73 + 2.147 2.110 -0.126
ENSG00000156858 E015 32.352725 0.0009249616 9.054801e-04 3.633101e-03 16 30651669 30651795 127 + 1.611 1.401 -0.720
ENSG00000156858 E016 347.146492 0.0009734926 1.699733e-02 4.492631e-02 16 30651796 30651964 169 + 2.529 2.473 -0.189
ENSG00000156858 E017 7.340521 0.0022524928 2.090681e-03 7.504515e-03 16 30652249 30652720 472 + 1.103 0.746 -1.350
ENSG00000156858 E018 406.817192 0.0002331240 8.453295e-04 3.421028e-03 16 30652721 30652842 122 + 2.603 2.539 -0.211
ENSG00000156858 E019 25.421302 0.0010472751 1.365905e-12 3.272627e-11 16 30652843 30652913 71 + 1.665 1.181 -1.674
ENSG00000156858 E020 551.420123 0.0006704387 2.722218e-02 6.644026e-02 16 30652914 30653103 190 + 2.718 2.677 -0.136
ENSG00000156858 E021 287.629043 0.0002315558 6.352436e-01 7.495910e-01 16 30653365 30653408 44 + 2.395 2.409 0.045
ENSG00000156858 E022 18.836910 0.0122153558 7.137727e-09 9.040293e-08 16 30654109 30654229 121 + 1.556 1.019 -1.889
ENSG00000156858 E023 415.492168 0.0016912901 3.560752e-01 5.004609e-01 16 30654230 30654339 110 + 2.543 2.573 0.099
ENSG00000156858 E024 343.901519 0.0021695978 3.344480e-02 7.867017e-02 16 30654629 30654820 192 + 2.431 2.502 0.236
ENSG00000156858 E025 399.251579 0.0005282967 3.799443e-03 1.258006e-02 16 30654821 30654997 177 + 2.497 2.565 0.226
ENSG00000156858 E026 589.341427 0.0001257217 5.915967e-06 4.142992e-05 16 30654998 30655140 143 + 2.659 2.737 0.260
ENSG00000156858 E027 550.698849 0.0001207655 1.171596e-02 3.285550e-02 16 30655141 30655214 74 + 2.655 2.700 0.151
ENSG00000156858 E028 36.161173 0.0149472300 1.007414e-06 8.349936e-06 16 30655215 30655350 136 + 1.763 1.365 -1.360
ENSG00000156858 E029 385.579174 0.0001486584 6.452898e-01 7.574124e-01 16 30655351 30655367 17 + 2.526 2.538 0.039
ENSG00000156858 E030 577.674534 0.0007908414 8.314666e-01 8.943684e-01 16 30655368 30655420 53 + 2.712 2.711 -0.002
ENSG00000156858 E031 36.368916 0.0008196373 9.120228e-12 1.913188e-10 16 30655421 30655501 81 + 1.766 1.366 -1.366
ENSG00000156858 E032 781.470775 0.0011639533 6.401110e-01 7.534130e-01 16 30655502 30655593 92 + 2.845 2.840 -0.016
ENSG00000156858 E033 12.116990 0.0018404117 3.733416e-03 1.239241e-02 16 30655719 30655867 149 + 1.256 0.978 -1.002
ENSG00000156858 E034 692.183443 0.0012764464 1.738468e-01 2.931939e-01 16 30655868 30655939 72 + 2.764 2.798 0.114
ENSG00000156858 E035 4.942357 0.0031706629 1.793492e-02 4.699585e-02 16 30655940 30655948 9 + 0.943 0.630 -1.251
ENSG00000156858 E036 1012.822053 0.0018072736 1.059554e-05 7.001370e-05 16 30656032 30656546 515 + 2.877 2.980 0.344