ENSG00000156853

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000287461 ENSG00000156853 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF689 protein_coding protein_coding 27.75298 43.39288 22.02293 2.834632 0.3721693 -0.9781291 26.44382 42.10916 19.86431 2.589059 0.2938078 -1.083572 0.9478833 0.9709 0.9023667 -0.06853333 0.0004232496 0.0004232496 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000156853 E001 2300.7018711 0.0069419033 1.132059e-06 9.292252e-06 16 30602558 30604539 1982 - 3.190 3.380 0.630
ENSG00000156853 E002 160.0470006 0.0024490985 7.605976e-01 8.446505e-01 16 30604540 30604617 78 - 2.106 2.196 0.303
ENSG00000156853 E003 229.6616161 0.0024064320 9.158184e-01 9.507578e-01 16 30604618 30604782 165 - 2.274 2.350 0.253
ENSG00000156853 E004 347.6462494 0.0001456258 9.099972e-07 7.621744e-06 16 30604783 30605046 264 - 2.520 2.500 -0.065
ENSG00000156853 E005 156.1216180 0.0002212553 5.985937e-04 2.528242e-03 16 30605047 30605079 33 - 2.179 2.154 -0.083
ENSG00000156853 E006 413.3266542 0.0011031125 9.546606e-07 7.951881e-06 16 30605080 30605447 368 - 2.604 2.572 -0.107
ENSG00000156853 E007 0.4470576 0.0217087029 2.149273e-01 3.443442e-01 16 30609436 30609524 89 - 0.274 0.094 -1.869
ENSG00000156853 E008 195.3983569 0.0066010697 1.827377e-04 8.896999e-04 16 30609525 30609629 105 - 2.319 2.229 -0.301
ENSG00000156853 E009 100.5167277 0.0052528832 1.154146e-02 3.244586e-02 16 30609630 30609638 9 - 2.003 1.956 -0.159
ENSG00000156853 E010 5.8054281 0.0028780537 1.951869e-05 1.214011e-04 16 30609742 30609836 95 - 1.062 0.590 -1.866
ENSG00000156853 E011 312.3677740 0.0081005104 1.093916e-02 3.101593e-02 16 30609837 30610201 365 - 2.483 2.451 -0.106
ENSG00000156853 E012 104.6876114 0.0054757759 6.443511e-04 2.695722e-03 16 30610202 30610210 9 - 2.048 1.959 -0.300
ENSG00000156853 E013 154.3492977 0.0057941188 5.104385e-03 1.621536e-02 16 30610211 30610365 155 - 2.188 2.141 -0.159
ENSG00000156853 E014 18.1426803 0.0249772761 2.856253e-01 4.259825e-01 16 30610366 30610587 222 - 1.279 1.232 -0.164
ENSG00000156853 E015 1.8143660 0.0148445205 3.413998e-01 4.852910e-01 16 30610588 30610782 195 - 0.505 0.388 -0.604
ENSG00000156853 E016 0.2214452 0.0405922147 1.000000e+00   16 30610783 30610985 203 - 0.000 0.094 11.037
ENSG00000156853 E017 0.3299976 0.0274424043 5.087917e-01   16 30623740 30624012 273 - 0.000 0.171 12.031