ENSG00000156471

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000517309 ENSG00000156471 HEK293_OSMI2_2hA HEK293_TMG_2hB PTDSS1 protein_coding protein_coding 104.0261 105.7208 100.2055 9.795031 1.792662 -0.07729096 59.246170 74.05213 47.945459 5.680394 0.7867726 -0.6270409 0.5729125 0.7025000 0.4789000 -0.2236 1.831804e-11 1.831804e-11 FALSE TRUE
ENST00000517557 ENSG00000156471 HEK293_OSMI2_2hA HEK293_TMG_2hB PTDSS1 protein_coding retained_intron 104.0261 105.7208 100.2055 9.795031 1.792662 -0.07729096 3.619941 0.00000 5.417669 0.000000 2.7423352 9.0841888 0.0331500 0.0000000 0.0532000 0.0532 1.730214e-01 1.831804e-11 FALSE TRUE
ENST00000518776 ENSG00000156471 HEK293_OSMI2_2hA HEK293_TMG_2hB PTDSS1 protein_coding processed_transcript 104.0261 105.7208 100.2055 9.795031 1.792662 -0.07729096 40.743800 31.31375 46.517126 4.341022 0.5583931 0.5708151 0.3897750 0.2941333 0.4646333 0.1705 1.964670e-07 1.831804e-11 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000156471 E001 6.631322 2.533775e-03 5.574214e-03 1.748689e-02 8 96261902 96261912 11 + 1.036 0.724 -1.196
ENSG00000156471 E002 257.216673 8.425523e-03 1.611419e-02 4.298790e-02 8 96261913 96261966 54 + 2.452 2.358 -0.314
ENSG00000156471 E003 868.748242 5.169546e-03 3.842883e-03 1.270102e-02 8 96261967 96262219 253 + 2.971 2.891 -0.266
ENSG00000156471 E004 699.634378 2.208458e-03 1.145555e-03 4.456119e-03 8 96273299 96273390 92 + 2.867 2.804 -0.207
ENSG00000156471 E005 0.000000       8 96283597 96283763 167 +      
ENSG00000156471 E006 656.521786 2.232566e-03 1.855966e-03 6.776404e-03 8 96284109 96284153 45 + 2.837 2.779 -0.195
ENSG00000156471 E007 1104.914270 1.108069e-03 5.236337e-04 2.248076e-03 8 96287022 96287146 125 + 3.053 3.012 -0.137
ENSG00000156471 E008 2.074434 2.962942e-02 6.215456e-01 7.385038e-01 8 96287147 96287388 242 + 0.527 0.458 -0.337
ENSG00000156471 E009 1039.823403 1.390067e-04 2.875898e-06 2.160482e-05 8 96295098 96295191 94 + 3.022 2.990 -0.105
ENSG00000156471 E010 885.319955 1.222724e-04 2.199584e-07 2.091681e-06 8 96295192 96295256 65 + 2.958 2.917 -0.137
ENSG00000156471 E011 1329.117259 3.643607e-04 1.110789e-05 7.303496e-05 8 96299694 96299845 152 + 3.128 3.095 -0.111
ENSG00000156471 E012 1285.244477 4.964994e-04 2.298663e-03 8.146112e-03 8 96304040 96304181 142 + 3.105 3.085 -0.066
ENSG00000156471 E013 1155.378971 8.716413e-04 3.281911e-02 7.744666e-02 8 96306444 96306556 113 + 3.054 3.041 -0.044
ENSG00000156471 E014 977.926051 1.627852e-04 5.243458e-01 6.578962e-01 8 96309557 96309622 66 + 2.964 2.981 0.056
ENSG00000156471 E015 1083.384508 8.740351e-05 4.241770e-01 5.671551e-01 8 96320246 96320345 100 + 3.010 3.026 0.054
ENSG00000156471 E016 930.519271 1.876759e-04 3.844360e-01 5.285789e-01 8 96330213 96330281 69 + 2.946 2.959 0.046
ENSG00000156471 E017 22.779525 7.960457e-04 2.572977e-02 6.338328e-02 8 96330317 96331025 709 + 1.444 1.308 -0.471
ENSG00000156471 E018 992.954561 2.450814e-04 8.282623e-01 8.920716e-01 8 96331026 96331095 70 + 2.968 2.990 0.073
ENSG00000156471 E019 812.403821 6.537574e-04 2.511566e-01 3.873214e-01 8 96333457 96333486 30 + 2.866 2.911 0.149
ENSG00000156471 E020 1459.957725 1.829948e-04 4.397302e-02 9.833163e-02 8 96333487 96333677 191 + 3.119 3.166 0.158
ENSG00000156471 E021 3611.009045 3.079017e-03 7.575295e-14 2.192357e-12 8 96333678 96336995 3318 + 3.402 3.603 0.665