ENSG00000156052

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000286548 ENSG00000156052 HEK293_OSMI2_2hA HEK293_TMG_2hB GNAQ protein_coding protein_coding 9.054216 3.25234 14.2535 0.2371708 0.248925 2.128349 6.852221 3.25234 10.795966 0.2371708 0.4710491 1.727849 0.8356000 1 0.7578000 -0.2422000 8.154406e-12 8.154406e-12 FALSE TRUE
MSTRG.32889.2 ENSG00000156052 HEK293_OSMI2_2hA HEK293_TMG_2hB GNAQ protein_coding   9.054216 3.25234 14.2535 0.2371708 0.248925 2.128349 2.123962 0.00000 3.373698 0.0000000 0.5932195 8.402457 0.1517542 0 0.2361667 0.2361667 3.155372e-10 8.154406e-12 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000156052 E001 914.8225551 0.0043410475 5.572837e-10 8.660848e-09 9 77716097 77721076 4980 - 2.840 3.018 0.591
ENSG00000156052 E002 119.0641320 0.0003877289 4.094971e-04 1.810846e-03 9 77721077 77721513 437 - 2.016 1.970 -0.154
ENSG00000156052 E003 73.1748101 0.0003685080 1.610097e-02 4.296050e-02 9 77728514 77728667 154 - 1.803 1.775 -0.093
ENSG00000156052 E004 72.7750060 0.0003352345 3.497612e-02 8.160649e-02 9 77794463 77794592 130 - 1.797 1.784 -0.043
ENSG00000156052 E005 36.2715858 0.0005698942 3.510469e-02 8.184499e-02 9 77797520 77797527 8 - 1.511 1.452 -0.200
ENSG00000156052 E006 64.8187935 0.0003688121 8.707272e-03 2.555407e-02 9 77797528 77797648 121 - 1.756 1.709 -0.160
ENSG00000156052 E007 66.7460251 0.0018683066 5.055634e-05 2.846031e-04 9 77815616 77815770 155 - 1.786 1.643 -0.482
ENSG00000156052 E008 73.4732348 0.0027396494 1.075292e-04 5.567240e-04 9 77922161 77922345 185 - 1.826 1.697 -0.436
ENSG00000156052 E009 0.0000000       9 77971145 77971197 53 -      
ENSG00000156052 E010 0.1515154 0.0431998867 1.000000e+00   9 78030515 78030604 90 - 0.066 0.001 -6.564
ENSG00000156052 E011 53.3815800 0.0004366163 1.814490e-06 1.424915e-05 9 78031100 78031811 712 - 1.703 1.499 -0.694