ENSG00000154930

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000323482 ENSG00000154930 HEK293_OSMI2_2hA HEK293_TMG_2hB ACSS1 protein_coding protein_coding 11.81146 20.07044 9.679299 0.8642469 0.3669454 -1.051327 1.540991 1.288841 1.680478 0.4538555 0.05679517 0.3802062 0.1380583 0.0630000 0.1744000 0.1114000 2.888385e-02 6.770728e-05 FALSE TRUE
ENST00000537502 ENSG00000154930 HEK293_OSMI2_2hA HEK293_TMG_2hB ACSS1 protein_coding protein_coding 11.81146 20.07044 9.679299 0.8642469 0.3669454 -1.051327 7.675204 14.660789 5.517230 0.5871772 0.36134854 -1.4083179 0.6354542 0.7307000 0.5691333 -0.1615667 6.770728e-05 6.770728e-05 FALSE TRUE
MSTRG.20488.3 ENSG00000154930 HEK293_OSMI2_2hA HEK293_TMG_2hB ACSS1 protein_coding   11.81146 20.07044 9.679299 0.8642469 0.3669454 -1.051327 2.273682 3.757297 2.058679 0.5416474 0.10532222 -0.8648200 0.1952000 0.1880667 0.2133667 0.0253000 7.951027e-01 6.770728e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000154930 E001 0.0000000       20 25006230 25006231 2 -      
ENSG00000154930 E002 0.8146179 0.1980312886 1.684534e-01 2.861588e-01 20 25006232 25006236 5 - 0.000 0.322 10.681
ENSG00000154930 E003 510.0858699 0.0026566275 5.207662e-11 9.641121e-10 20 25006237 25006925 689 - 2.513 2.710 0.656
ENSG00000154930 E004 490.1455551 0.0001386333 3.502318e-15 1.233049e-13 20 25006926 25007615 690 - 2.528 2.681 0.508
ENSG00000154930 E005 243.7609061 0.0002360416 5.896518e-02 1.248620e-01 20 25007616 25007941 326 - 2.363 2.333 -0.098
ENSG00000154930 E006 141.3907758 0.0003580170 4.235234e-02 9.536452e-02 20 25009270 25009388 119 - 2.139 2.090 -0.164
ENSG00000154930 E007 11.6118730 0.0124499960 9.396365e-01 9.660863e-01 20 25009389 25012326 2938 - 1.056 1.063 0.026
ENSG00000154930 E008 106.3764404 0.0002925526 6.713025e-03 2.047729e-02 20 25012601 25012664 64 - 2.041 1.959 -0.275
ENSG00000154930 E009 88.9129197 0.0003372287 1.688506e-01 2.866792e-01 20 25012812 25012856 45 - 1.931 1.892 -0.132
ENSG00000154930 E010 107.3399373 0.0002709979 1.088202e-02 3.088580e-02 20 25012857 25012939 83 - 2.039 1.963 -0.254
ENSG00000154930 E011 103.8171118 0.0002980643 1.261582e-04 6.419977e-04 20 25013536 25013662 127 - 2.055 1.932 -0.413
ENSG00000154930 E012 100.0923342 0.0003184342 1.349002e-02 3.705604e-02 20 25013961 25014073 113 - 2.010 1.933 -0.256
ENSG00000154930 E013 86.8950989 0.0003405447 1.104960e-01 2.062520e-01 20 25015138 25015230 93 - 1.931 1.883 -0.163
ENSG00000154930 E014 90.9547654 0.0003240320 5.341746e-01 6.662827e-01 20 25020010 25020147 138 - 1.921 1.912 -0.030
ENSG00000154930 E015 88.4843771 0.0003532079 1.174822e-01 2.163501e-01 20 25021389 25021536 148 - 1.938 1.892 -0.157
ENSG00000154930 E016 88.9761916 0.0022283435 3.110254e-01 4.534757e-01 20 25022940 25023092 153 - 1.926 1.897 -0.099
ENSG00000154930 E017 85.3464872 0.0010775957 1.323278e-01 2.373383e-01 20 25023466 25023641 176 - 1.921 1.873 -0.163
ENSG00000154930 E018 126.8222164 0.0003006370 3.669934e-03 1.221036e-02 20 25030759 25030958 200 - 2.114 2.034 -0.270
ENSG00000154930 E019 0.0000000       20 25032369 25032706 338 -      
ENSG00000154930 E020 1.2103937 0.0110290726 2.981471e-01 4.395536e-01 20 25045938 25046872 935 - 0.168 0.371 1.518
ENSG00000154930 E021 88.1031169 0.0003653409 1.453558e-04 7.270744e-04 20 25048085 25048181 97 - 1.994 1.860 -0.448
ENSG00000154930 E022 78.5282351 0.0044695486 3.846369e-07 3.478865e-06 20 25057769 25058980 1212 - 2.008 1.767 -0.810