ENSG00000154781

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000383794 ENSG00000154781 HEK293_OSMI2_2hA HEK293_TMG_2hB CCDC174 protein_coding protein_coding 11.64202 7.905331 15.28983 0.2832126 0.7367427 0.9507935 6.481601 5.5395807 8.168174 0.29391013 0.1128998 0.5593999 0.58141250 0.700566667 0.5365333 -0.16403333 0.006657484 0.006657484 FALSE TRUE
ENST00000463438 ENSG00000154781 HEK293_OSMI2_2hA HEK293_TMG_2hB CCDC174 protein_coding retained_intron 11.64202 7.905331 15.28983 0.2832126 0.7367427 0.9507935 1.056496 0.0649245 1.720111 0.06492450 0.3219214 4.5292830 0.07537917 0.008833333 0.1115667 0.10273333 0.015257726 0.006657484 FALSE TRUE
ENST00000465759 ENSG00000154781 HEK293_OSMI2_2hA HEK293_TMG_2hB CCDC174 protein_coding retained_intron 11.64202 7.905331 15.28983 0.2832126 0.7367427 0.9507935 1.231530 0.5775706 1.543593 0.12419703 0.2217044 1.4027749 0.10162917 0.072266667 0.1003667 0.02810000 0.506602654 0.006657484 FALSE TRUE
MSTRG.22516.1 ENSG00000154781 HEK293_OSMI2_2hA HEK293_TMG_2hB CCDC174 protein_coding   11.64202 7.905331 15.28983 0.2832126 0.7367427 0.9507935 2.409245 1.6599169 3.554999 0.08411221 0.2951970 1.0941255 0.20143333 0.210500000 0.2319667 0.02146667 0.743372085 0.006657484 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000154781 E001 2.110013 0.0069010418 3.211107e-02 7.607129e-02 3 14651669 14651761 93 + 0.628 0.251 -2.054
ENSG00000154781 E002 2.078678 0.0069296195 3.177804e-02 7.542194e-02 3 14651762 14651763 2 + 0.628 0.251 -2.055
ENSG00000154781 E003 7.644764 0.0023175319 4.972773e-02 1.086782e-01 3 14651764 14651772 9 + 1.020 0.785 -0.897
ENSG00000154781 E004 57.364059 0.0004201161 3.569913e-05 2.082490e-04 3 14651773 14651878 106 + 1.844 1.648 -0.663
ENSG00000154781 E005 86.073804 0.0005705979 1.622640e-06 1.287504e-05 3 14654426 14654530 105 + 2.015 1.827 -0.634
ENSG00000154781 E006 97.685940 0.0004682525 1.013658e-02 2.910011e-02 3 14655529 14655629 101 + 2.033 1.940 -0.313
ENSG00000154781 E007 71.078796 0.0003696637 9.597534e-01 9.787039e-01 3 14658871 14658929 59 + 1.857 1.858 0.005
ENSG00000154781 E008 108.298273 0.0051932589 4.453771e-01 5.869263e-01 3 14661530 14661707 178 + 2.055 2.015 -0.133
ENSG00000154781 E009 2.191584 0.0143378069 3.517764e-02 8.197977e-02 3 14661708 14661900 193 + 0.628 0.251 -2.052
ENSG00000154781 E010 74.120558 0.0028877230 5.156754e-01 6.503155e-01 3 14665028 14665123 96 + 1.890 1.858 -0.109
ENSG00000154781 E011 81.736431 0.0003897942 2.227133e-01 3.536128e-01 3 14666805 14666947 143 + 1.899 1.945 0.156
ENSG00000154781 E012 8.938365 0.0081718316 5.613184e-01 6.891680e-01 3 14666948 14667298 351 + 1.031 0.964 -0.248
ENSG00000154781 E013 94.793050 0.0021603740 1.558708e-01 2.696095e-01 3 14667424 14667518 95 + 1.956 2.014 0.194
ENSG00000154781 E014 1.795340 0.0688560588 3.106916e-01 4.531244e-01 3 14668002 14668048 47 + 0.536 0.336 -1.061
ENSG00000154781 E015 133.630452 0.0002603835 7.529034e-04 3.089819e-03 3 14668049 14668181 133 + 2.082 2.182 0.332
ENSG00000154781 E016 125.572894 0.0006179539 3.282664e-05 1.934522e-04 3 14669934 14670086 153 + 2.040 2.171 0.440
ENSG00000154781 E017 210.496243 0.0002218708 5.776677e-02 1.228006e-01 3 14670896 14671746 851 + 2.304 2.348 0.145
ENSG00000154781 E018 145.740420 0.0003255749 2.975794e-01 4.389420e-01 3 14671747 14672659 913 + 2.153 2.183 0.099
ENSG00000154781 E019 18.854299 0.0010186830 6.591925e-04 2.749313e-03 3 14672680 14672829 150 + 1.400 1.118 -0.990