ENSG00000154743

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000402228 ENSG00000154743 HEK293_OSMI2_2hA HEK293_TMG_2hB TSEN2 protein_coding protein_coding 11.9899 9.480932 15.31155 1.064067 0.2357221 0.6909409 0.7644555 0.3029544 0.5012967 0.30295444 0.26241711 0.7082081 0.05813750 0.02626667 0.03326667 0.007000000 7.415547e-01 3.128489e-05 FALSE TRUE
ENST00000455118 ENSG00000154743 HEK293_OSMI2_2hA HEK293_TMG_2hB TSEN2 protein_coding nonsense_mediated_decay 11.9899 9.480932 15.31155 1.064067 0.2357221 0.6909409 0.6937867 0.4588853 0.9181931 0.01774692 0.14243675 0.9851899 0.06140833 0.05000000 0.05996667 0.009966667 7.970437e-01 3.128489e-05 TRUE TRUE
ENST00000473755 ENSG00000154743 HEK293_OSMI2_2hA HEK293_TMG_2hB TSEN2 protein_coding retained_intron 11.9899 9.480932 15.31155 1.064067 0.2357221 0.6909409 0.7493895 0.4017902 0.9447253 0.02150166 0.05456364 1.2131762 0.06610000 0.04316667 0.06180000 0.018633333 3.607215e-01 3.128489e-05 TRUE TRUE
ENST00000679670 ENSG00000154743 HEK293_OSMI2_2hA HEK293_TMG_2hB TSEN2 protein_coding nonsense_mediated_decay 11.9899 9.480932 15.31155 1.064067 0.2357221 0.6909409 0.5609810 0.0000000 0.7661619 0.00000000 0.76616186 6.2782856 0.03809583 0.00000000 0.05076667 0.050766667 9.120718e-01 3.128489e-05 TRUE TRUE
ENST00000679699 ENSG00000154743 HEK293_OSMI2_2hA HEK293_TMG_2hB TSEN2 protein_coding protein_coding 11.9899 9.480932 15.31155 1.064067 0.2357221 0.6909409 1.6954273 1.4748403 3.2695301 0.21300056 0.07209828 1.1431814 0.13238333 0.15990000 0.21380000 0.053900000 3.996435e-01 3.128489e-05 FALSE TRUE
ENST00000681482 ENSG00000154743 HEK293_OSMI2_2hA HEK293_TMG_2hB TSEN2 protein_coding nonsense_mediated_decay 11.9899 9.480932 15.31155 1.064067 0.2357221 0.6909409 2.7103933 4.2641966 2.9979546 0.51215888 0.86549146 -0.5068705 0.24546667 0.44943333 0.19500000 -0.254433333 3.546309e-02 3.128489e-05 TRUE TRUE
MSTRG.22502.1 ENSG00000154743 HEK293_OSMI2_2hA HEK293_TMG_2hB TSEN2 protein_coding   11.9899 9.480932 15.31155 1.064067 0.2357221 0.6909409 0.7089198 0.1967025 1.1769945 0.03660524 0.07431597 2.5216852 0.05585417 0.02043333 0.07676667 0.056333333 3.128489e-05 3.128489e-05 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000154743 E001 0.5202097 0.0215016700 7.846055e-01 8.618328e-01 3 12484421 12484434 14 + 0.191 0.141 -0.527
ENSG00000154743 E002 0.6717251 0.0191006678 4.952391e-01 6.320802e-01 3 12484435 12484442 8 + 0.262 0.141 -1.112
ENSG00000154743 E003 2.7398041 0.0059561457 5.873241e-01 7.106278e-01 3 12484443 12484479 37 + 0.606 0.518 -0.403
ENSG00000154743 E004 5.4001124 0.0030615649 9.311731e-01 9.606497e-01 3 12484480 12484490 11 + 0.815 0.804 -0.044
ENSG00000154743 E005 7.7092165 0.0021633643 7.955195e-01 8.694102e-01 3 12484491 12484496 6 + 0.927 0.956 0.108
ENSG00000154743 E006 13.0707496 0.0013573348 4.058288e-01 5.494862e-01 3 12484497 12484504 8 + 1.187 1.110 -0.276
ENSG00000154743 E007 17.0617496 0.0011179858 2.743272e-01 4.134616e-01 3 12484505 12484510 6 + 1.303 1.213 -0.316
ENSG00000154743 E008 20.8688671 0.0018778793 2.889810e-01 4.296444e-01 3 12484511 12484517 7 + 1.384 1.304 -0.280
ENSG00000154743 E009 1.1007987 0.0137016050 9.603752e-01 9.790363e-01 3 12484518 12484526 9 + 0.323 0.332 0.056
ENSG00000154743 E010 3.0173623 0.0053060202 9.843423e-01 9.942909e-01 3 12484527 12484553 27 + 0.606 0.609 0.011
ENSG00000154743 E011 2.8690691 0.0052033144 8.011528e-01 8.732852e-01 3 12484554 12484554 1 + 0.606 0.566 -0.181
ENSG00000154743 E012 3.4957548 0.0046323295 9.290155e-01 9.593642e-01 3 12484555 12484562 8 + 0.662 0.648 -0.059
ENSG00000154743 E013 5.5670561 0.0029619448 5.762901e-01 7.017698e-01 3 12484563 12484604 42 + 0.850 0.777 -0.288
ENSG00000154743 E014 6.6053119 0.0025056831 6.214975e-01 7.384684e-01 3 12484605 12484628 24 + 0.913 0.853 -0.230
ENSG00000154743 E015 14.2260139 0.0013027284 1.012190e-01 1.923324e-01 3 12484629 12484880 252 + 1.245 1.097 -0.529
ENSG00000154743 E016 3.9932641 0.0040003830 3.187060e-01 4.616718e-01 3 12484881 12485191 311 + 0.756 0.609 -0.616
ENSG00000154743 E017 10.1639528 0.0018358247 6.844336e-02 1.407967e-01 3 12487043 12488346 1304 + 1.128 0.938 -0.699
ENSG00000154743 E018 36.2412413 0.0011116697 7.054032e-01 8.037707e-01 3 12489784 12489800 17 + 1.586 1.563 -0.079
ENSG00000154743 E019 92.5158586 0.0117721041 1.817271e-01 3.033996e-01 3 12489801 12489989 189 + 2.012 1.925 -0.292
ENSG00000154743 E020 76.2232741 0.0005087897 8.058747e-02 1.604726e-01 3 12492136 12492217 82 + 1.927 1.856 -0.238
ENSG00000154743 E021 77.9936753 0.0003389964 2.747404e-01 4.139460e-01 3 12496518 12496554 37 + 1.922 1.879 -0.146
ENSG00000154743 E022 0.2903454 0.2980988199 4.005706e-01   3 12501668 12501724 57 + 0.191 0.000 -9.658
ENSG00000154743 E023 147.5977791 0.0005681164 1.894848e-01 3.130982e-01 3 12503262 12503469 208 + 2.196 2.158 -0.128
ENSG00000154743 E024 124.6297181 0.0002426690 4.747291e-01 6.135394e-01 3 12503470 12503646 177 + 2.114 2.092 -0.075
ENSG00000154743 E025 94.1924976 0.0003215590 5.592743e-01 6.874029e-01 3 12503647 12503784 138 + 1.974 1.994 0.068
ENSG00000154743 E026 4.2229249 0.0053797702 4.172175e-01 5.605847e-01 3 12503785 12505153 1369 + 0.662 0.777 0.470
ENSG00000154743 E027 55.5710184 0.0004445782 4.687943e-01 6.081678e-01 3 12505154 12505231 78 + 1.740 1.772 0.111
ENSG00000154743 E028 2.2518587 0.0066226866 2.607933e-01 3.983883e-01 3 12505232 12505766 535 + 0.425 0.609 0.885
ENSG00000154743 E029 2.0346679 0.0071201404 8.016620e-01 8.736185e-01 3 12506708 12506739 32 + 0.507 0.465 -0.208
ENSG00000154743 E030 4.1621839 0.0038941219 1.947489e-01 3.196857e-01 3 12506740 12506835 96 + 0.796 0.609 -0.777
ENSG00000154743 E031 46.8682675 0.0008675266 1.555387e-01 2.691187e-01 3 12516611 12516661 51 + 1.647 1.719 0.242
ENSG00000154743 E032 1.4726134 0.0168274537 4.230812e-01 5.662093e-01 3 12516662 12517033 372 + 0.323 0.465 0.793
ENSG00000154743 E033 2.5623662 0.0115382987 3.971960e-01 5.409788e-01 3 12517889 12519058 1170 + 0.607 0.465 -0.666
ENSG00000154743 E034 91.0714979 0.0008396672 2.520724e-01 3.883953e-01 3 12519059 12519197 139 + 1.947 1.989 0.139
ENSG00000154743 E035 58.1414589 0.0027713279 5.068955e-01 6.425248e-01 3 12528888 12528924 37 + 1.759 1.791 0.108
ENSG00000154743 E036 91.5118299 0.0074313641 3.933181e-01 5.372196e-01 3 12529762 12529873 112 + 1.948 1.986 0.126
ENSG00000154743 E037 22.4190483 0.0008927166 1.733876e-01 2.925939e-01 3 12529874 12530854 981 + 1.413 1.313 -0.348
ENSG00000154743 E038 5.7475895 0.0042929253 4.948000e-01 6.317028e-01 3 12530855 12530941 87 + 0.867 0.777 -0.351
ENSG00000154743 E039 17.4090522 0.0013975225 3.366418e-02 7.908606e-02 3 12530942 12531538 597 + 1.337 1.159 -0.628
ENSG00000154743 E040 4.1155305 0.0992797646 4.422017e-01 5.838545e-01 3 12531539 12531569 31 + 0.636 0.776 0.581
ENSG00000154743 E041 90.0948848 0.0024824490 8.205599e-01 8.868361e-01 3 12531570 12531659 90 + 1.958 1.964 0.019
ENSG00000154743 E042 3.7007261 0.0129731288 4.519822e-01 5.928860e-01 3 12531660 12532661 1002 + 0.606 0.718 0.474
ENSG00000154743 E043 86.7391217 0.0003387896 2.509845e-02 6.207913e-02 3 12532662 12532773 112 + 1.906 1.989 0.278
ENSG00000154743 E044 99.0464395 0.0003591474 7.444879e-02 1.506526e-01 3 12532774 12533659 886 + 2.035 1.973 -0.209
ENSG00000154743 E045 9.0065657 0.0022040254 5.601840e-04 2.385574e-03 3 12536303 12536814 512 + 1.146 0.748 -1.499
ENSG00000154743 E046 11.3312201 0.0065646869 2.552171e-01 3.920450e-01 3 12538802 12539160 359 + 1.146 1.024 -0.440
ENSG00000154743 E047 76.5523366 0.0003791877 2.759025e-19 1.623258e-17 3 12539161 12539658 498 + 1.691 2.052 1.217
ENSG00000154743 E048 0.9179615 0.0136874041 6.964091e-01 7.967716e-01 3 12541269 12541549 281 + 0.262 0.332 0.469