ENSG00000154727

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000354828 ENSG00000154727 HEK293_OSMI2_2hA HEK293_TMG_2hB GABPA protein_coding protein_coding 11.36798 2.414986 20.16778 0.1340413 1.048872 3.056719 2.957525 0.2507303 6.005503 0.09232211 0.4066663 4.528055 0.1850625 0.1073667 0.2975667 0.1902 1.054720e-02 1.045415e-06 FALSE TRUE
ENST00000400075 ENSG00000154727 HEK293_OSMI2_2hA HEK293_TMG_2hB GABPA protein_coding protein_coding 11.36798 2.414986 20.16778 0.1340413 1.048872 3.056719 4.771052 0.6608125 8.879195 0.02743242 0.3286762 3.728071 0.3950458 0.2764000 0.4421000 0.1657 2.082812e-02 1.045415e-06 FALSE TRUE
MSTRG.21177.4 ENSG00000154727 HEK293_OSMI2_2hA HEK293_TMG_2hB GABPA protein_coding   11.36798 2.414986 20.16778 0.1340413 1.048872 3.056719 3.008598 1.5034435 4.794445 0.23448533 0.2641558 1.666535 0.3881292 0.6162667 0.2377667 -0.3785 1.045415e-06 1.045415e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000154727 E001 1.9229976 0.0073840141 1.649407e-01 2.816233e-01 21 25734570 25734971 402 + 0.357 0.000 -10.617
ENSG00000154727 E002 28.6498274 0.0006662106 4.006274e-03 1.315954e-02 21 25734972 25735070 99 + 1.276 1.071 -0.728
ENSG00000154727 E003 31.1409986 0.0062419727 5.626668e-01 6.902336e-01 21 25735071 25735388 318 + 1.283 1.354 0.251
ENSG00000154727 E004 38.4990014 0.0005861321 1.355468e-02 3.720465e-02 21 25735389 25735578 190 + 1.390 1.286 -0.362
ENSG00000154727 E005 17.1760090 0.0017791792 7.394045e-03 2.224307e-02 21 25739749 25740032 284 + 0.984 1.394 1.459
ENSG00000154727 E006 94.6825250 0.0003541660 1.084226e-04 5.608132e-04 21 25741573 25741675 103 + 1.770 1.666 -0.352
ENSG00000154727 E007 97.9674101 0.0002757453 7.382680e-06 5.054485e-05 21 25745210 25745354 145 + 1.787 1.645 -0.482
ENSG00000154727 E008 89.6535732 0.0002961499 3.007770e-03 1.028878e-02 21 25749036 25749120 85 + 1.741 1.695 -0.156
ENSG00000154727 E009 70.5765950 0.0003389823 7.220581e-04 2.977877e-03 21 25751989 25752234 246 + 1.644 1.537 -0.366
ENSG00000154727 E010 0.1451727 0.0428248963 6.959217e-01   21 25752235 25752348 114 + 0.041 0.000 -6.915
ENSG00000154727 E011 75.0020187 0.0003236866 1.678294e-03 6.213556e-03 21 25758010 25758204 195 + 1.668 1.588 -0.271
ENSG00000154727 E012 70.2948294 0.0003909069 1.272232e-02 3.525910e-02 21 25762312 25762365 54 + 1.637 1.600 -0.124
ENSG00000154727 E013 106.6759273 0.0003650291 1.309273e-02 3.612291e-02 21 25764210 25764350 141 + 1.810 1.811 0.001
ENSG00000154727 E014 135.6162959 0.0002404289 8.981139e-04 3.607748e-03 21 25764595 25764787 193 + 1.916 1.890 -0.088
ENSG00000154727 E015 817.4164551 0.0102888265 1.726849e-08 2.037661e-07 21 25769004 25772460 3457 + 2.652 2.936 0.944