ENSG00000154640

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000339775 ENSG00000154640 HEK293_OSMI2_2hA HEK293_TMG_2hB BTG3 protein_coding protein_coding 22.88205 11.88688 37.54303 2.187614 1.346179 1.658346 1.872003 1.0381436 2.390159 0.1272416 0.1229148 1.195293 0.08367917 0.09113333 0.0638000 -0.02733333 0.397170600 3.286992e-11 FALSE TRUE
ENST00000348354 ENSG00000154640 HEK293_OSMI2_2hA HEK293_TMG_2hB BTG3 protein_coding protein_coding 22.88205 11.88688 37.54303 2.187614 1.346179 1.658346 14.224329 9.3806068 23.227056 1.4236650 0.7274891 1.307137 0.68157083 0.79913333 0.6188333 -0.18030000 0.007873605 3.286992e-11 FALSE TRUE
MSTRG.21152.4 ENSG00000154640 HEK293_OSMI2_2hA HEK293_TMG_2hB BTG3 protein_coding   22.88205 11.88688 37.54303 2.187614 1.346179 1.658346 4.635177 0.7676667 6.842837 0.7676667 0.2500457 3.139477 0.15483750 0.04746667 0.1822667 0.13480000 0.132487094 3.286992e-11 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000154640 E001 0.1451727 0.0442849379 1.000000e+00   21 17588464 17588537 74 - 0.086 0.000 -9.620
ENSG00000154640 E002 144.0493270 0.0006498005 3.888724e-14 1.171981e-12 21 17593653 17593969 317 - 2.058 2.278 0.736
ENSG00000154640 E003 105.7805399 0.0064473437 1.268150e-03 4.871096e-03 21 17593970 17594053 84 - 1.953 2.114 0.540
ENSG00000154640 E004 85.5824359 0.0087271910 1.758972e-01 2.958590e-01 21 17594054 17594089 36 - 1.896 1.976 0.269
ENSG00000154640 E005 257.5751507 0.0013587823 7.000233e-02 1.433758e-01 21 17594090 17594332 243 - 2.385 2.423 0.126
ENSG00000154640 E006 171.4689538 0.0016650678 9.521480e-01 9.740405e-01 21 17598617 17598720 104 - 2.226 2.213 -0.041
ENSG00000154640 E007 166.4694284 0.0002219266 1.180679e-01 2.171655e-01 21 17598721 17598824 104 - 2.227 2.175 -0.173
ENSG00000154640 E008 1.7819870 0.0085583583 3.136557e-01 4.562748e-01 21 17598825 17599091 267 - 0.364 0.534 0.885
ENSG00000154640 E009 14.0789803 0.0012669868 8.699962e-01 9.203655e-01 21 17604145 17604276 132 - 1.156 1.163 0.026
ENSG00000154640 E010 166.6887988 0.0002736683 6.450414e-02 1.342221e-01 21 17604860 17604997 138 - 2.229 2.168 -0.204
ENSG00000154640 E011 4.4297919 0.0041314219 1.862500e-03 6.797114e-03 21 17604998 17605133 136 - 0.839 0.294 -2.605
ENSG00000154640 E012 3.8459952 0.0041247631 2.836908e-05 1.696959e-04 21 17608775 17608971 197 - 0.825 0.000 -14.319
ENSG00000154640 E013 94.0377825 0.0072559550 3.979013e-01 5.416506e-01 21 17608972 17609006 35 - 1.983 1.916 -0.225
ENSG00000154640 E014 148.6790814 0.0042027739 2.536339e-04 1.190333e-03 21 17609007 17609152 146 - 2.212 2.036 -0.590
ENSG00000154640 E015 1.1929815 0.0156252119 3.323345e-02 7.826438e-02 21 17610776 17610819 44 - 0.439 0.000 -12.619
ENSG00000154640 E016 6.8571390 0.0025427016 4.155822e-01 5.589492e-01 21 17611657 17611774 118 - 0.914 0.801 -0.436
ENSG00000154640 E017 103.7096492 0.0165272662 1.337690e-04 6.756824e-04 21 17612699 17612945 247 - 2.087 1.776 -1.046