ENSG00000154518

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000284727 ENSG00000154518 HEK293_OSMI2_2hA HEK293_TMG_2hB ATP5MC3 protein_coding protein_coding 623.6937 1173.807 303.6387 57.8637 2.935714 -1.950732 17.85135 10.16517 32.79044 1.255341 1.305972 1.688662 0.0456750 0.008666667 0.10793333 0.09926667 1.073149e-53 1.073149e-53 FALSE  
ENST00000409194 ENSG00000154518 HEK293_OSMI2_2hA HEK293_TMG_2hB ATP5MC3 protein_coding protein_coding 623.6937 1173.807 303.6387 57.8637 2.935714 -1.950732 489.26188 948.25333 218.10137 58.569355 4.103198 -2.120223 0.7670917 0.806833333 0.71823333 -0.08860000 2.271200e-03 1.073149e-53 FALSE  
ENST00000472782 ENSG00000154518 HEK293_OSMI2_2hA HEK293_TMG_2hB ATP5MC3 protein_coding retained_intron 623.6937 1173.807 303.6387 57.8637 2.935714 -1.950732 40.43855 84.10417 15.51122 4.461102 0.450947 -2.438107 0.0594875 0.071833333 0.05113333 -0.02070000 7.873605e-03 1.073149e-53    
ENST00000497075 ENSG00000154518 HEK293_OSMI2_2hA HEK293_TMG_2hB ATP5MC3 protein_coding retained_intron 623.6937 1173.807 303.6387 57.8637 2.935714 -1.950732 60.71729 107.92527 28.07573 5.383770 1.675130 -1.942257 0.1010125 0.092666667 0.09256667 -0.00010000 1.000000e+00 1.073149e-53 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000154518 E001 32.76416 0.0006230576 8.168556e-36 2.102593e-33 2 175176258 175176492 235 - 1.912 1.157 -2.595
ENSG00000154518 E002 522.84480 0.0109201995 1.447087e-93 3.655284e-90 2 175176493 175178066 1574 - 3.226 2.095 -3.766
ENSG00000154518 E003 151.28758 0.0172159786 9.018617e-04 3.620116e-03 2 175178067 175178144 78 - 2.246 2.021 -0.751
ENSG00000154518 E004 729.07849 0.0026022501 7.933935e-01 8.678979e-01 2 175178145 175178185 41 - 2.752 2.749 -0.010
ENSG00000154518 E005 8351.11387 0.0011648294 1.338462e-10 2.314840e-09 2 175178186 175178402 217 - 3.726 3.822 0.320
ENSG00000154518 E006 109.01263 0.0062830347 1.216469e-18 6.645226e-17 2 175178403 175179056 654 - 2.260 1.807 -1.518
ENSG00000154518 E007 11889.15472 0.0003277991 6.169254e-23 5.430979e-21 2 175179057 175179250 194 - 3.896 3.973 0.254
ENSG00000154518 E008 25.47722 0.0007680313 6.308504e-05 3.465945e-04 2 175179645 175180097 453 - 1.540 1.243 -1.026
ENSG00000154518 E009 8025.06565 0.0002202822 2.083473e-01 3.364970e-01 2 175180098 175180178 81 - 3.795 3.788 -0.023
ENSG00000154518 E010 7254.45545 0.0006280913 1.585018e-03 5.910266e-03 2 175181355 175181466 112 - 3.772 3.739 -0.112
ENSG00000154518 E011 100.00898 0.0002756674 8.383286e-04 3.395960e-03 2 175181467 175181483 17 - 2.000 1.865 -0.455
ENSG00000154518 E012 51.11641 0.0011191213 7.180088e-02 1.463464e-01 2 175181484 175181655 172 - 1.687 1.582 -0.355
ENSG00000154518 E013 3264.94819 0.0037497636 1.175147e-01 2.163939e-01 2 175181656 175181854 199 - 3.434 3.390 -0.147
ENSG00000154518 E014 0.00000       2 175181982 175182035 54 -