ENSG00000154328

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000284503 ENSG00000154328 HEK293_OSMI2_2hA HEK293_TMG_2hB NEIL2 protein_coding protein_coding 8.999884 15.12382 6.487492 0.1667087 0.5854546 -1.219821 1.1607718 1.7108301 1.2024064 0.15326920 0.0734655 -0.5052313 0.13917917 0.11333333 0.18970000 0.07636667 0.12956489 0.02235995 FALSE  
ENST00000403422 ENSG00000154328 HEK293_OSMI2_2hA HEK293_TMG_2hB NEIL2 protein_coding protein_coding 8.999884 15.12382 6.487492 0.1667087 0.5854546 -1.219821 1.9216604 4.7753046 1.1585174 0.99803009 0.2506403 -2.0339315 0.19512917 0.31493333 0.17893333 -0.13600000 0.29070307 0.02235995 FALSE  
ENST00000436750 ENSG00000154328 HEK293_OSMI2_2hA HEK293_TMG_2hB NEIL2 protein_coding protein_coding 8.999884 15.12382 6.487492 0.1667087 0.5854546 -1.219821 2.9837084 3.8491369 2.5012158 0.44716189 0.1864759 -0.6198922 0.33785833 0.25450000 0.38760000 0.13310000 0.05695668 0.02235995 FALSE  
ENST00000455213 ENSG00000154328 HEK293_OSMI2_2hA HEK293_TMG_2hB NEIL2 protein_coding protein_coding 8.999884 15.12382 6.487492 0.1667087 0.5854546 -1.219821 0.6288413 0.7327634 0.0851168 0.44571815 0.0851168 -2.9651307 0.07722917 0.04790000 0.01456667 -0.03333333 0.66002217 0.02235995 FALSE  
ENST00000524741 ENSG00000154328 HEK293_OSMI2_2hA HEK293_TMG_2hB NEIL2 protein_coding retained_intron 8.999884 15.12382 6.487492 0.1667087 0.5854546 -1.219821 0.5995685 0.3150358 0.5690947 0.03234855 0.1449553 0.8332004 0.07606250 0.02083333 0.09280000 0.07196667 0.02235995 0.02235995 FALSE  
ENST00000528113 ENSG00000154328 HEK293_OSMI2_2hA HEK293_TMG_2hB NEIL2 protein_coding nonsense_mediated_decay 8.999884 15.12382 6.487492 0.1667087 0.5854546 -1.219821 0.4844933 1.7208870 0.6010275 1.17128171 0.6010275 -1.5022023 0.05150000 0.11236667 0.07850000 -0.03386667 0.66757550 0.02235995 FALSE  
MSTRG.31145.3 ENSG00000154328 HEK293_OSMI2_2hA HEK293_TMG_2hB NEIL2 protein_coding   8.999884 15.12382 6.487492 0.1667087 0.5854546 -1.219821 0.8590083 1.1857417 0.2338993 1.18574168 0.2338993 -2.2935483 0.08628750 0.08013333 0.04006667 -0.04006667 0.96029480 0.02235995 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000154328 E001 2.324910 0.0161569211 0.2765102977 0.4159741952 8 11769639 11769698 60 + 0.590 0.441 -0.718
ENSG00000154328 E002 2.769891 0.0058241410 0.1156544115 0.2137660742 8 11769699 11769705 7 + 0.686 0.474 -0.963
ENSG00000154328 E003 3.212782 0.0052376957 0.2214945968 0.3521972677 8 11769706 11769708 3 + 0.686 0.534 -0.673
ENSG00000154328 E004 3.212782 0.0052376957 0.2214945968 0.3521972677 8 11769709 11769709 1 + 0.686 0.534 -0.673
ENSG00000154328 E005 22.627568 0.0071898648 0.2571091331 0.3941491187 8 11769710 11769791 82 + 1.356 1.310 -0.161
ENSG00000154328 E006 12.518663 0.0034002126 0.1199062813 0.2198382394 8 11769792 11770010 219 + 1.161 1.054 -0.386
ENSG00000154328 E007 12.107786 0.0018512065 0.0570144914 0.1214862876 8 11770011 11770173 163 + 1.175 1.037 -0.500
ENSG00000154328 E008 20.563025 0.0091838286 0.9647412774 0.9817750184 8 11770174 11770335 162 + 1.252 1.306 0.191
ENSG00000154328 E009 43.413435 0.0006612950 0.1221867834 0.2230651770 8 11771446 11771585 140 + 1.623 1.586 -0.124
ENSG00000154328 E010 1.499708 0.4010058042 0.3326102942 0.4762849486 8 11771586 11771590 5 + 0.466 0.325 -0.792
ENSG00000154328 E011 100.722813 0.0090138169 0.7677403981 0.8499109758 8 11779598 11779950 353 + 1.926 1.965 0.129
ENSG00000154328 E012 6.428803 0.0030333106 0.0001897915 0.0009203237 8 11782612 11783202 591 + 1.082 0.675 -1.569
ENSG00000154328 E013 102.864647 0.0004361994 0.0555502760 0.1189214313 8 11783203 11783399 197 + 1.978 1.960 -0.060
ENSG00000154328 E014 482.384077 0.0091279769 0.0096420406 0.0278854655 8 11785963 11787345 1383 + 2.549 2.663 0.379