ENSG00000154217

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000580974 ENSG00000154217 HEK293_OSMI2_2hA HEK293_TMG_2hB PITPNC1 protein_coding protein_coding 2.372199 3.102755 2.466599 0.1860709 0.2208472 -0.3298318 1.7815664 2.48814255 1.5040283 0.13876020 0.05400662 -0.7224637 0.75322083 0.80236667 0.6163333 -0.1860333 0.007393045 0.007393045 FALSE TRUE
ENST00000581322 ENSG00000154217 HEK293_OSMI2_2hA HEK293_TMG_2hB PITPNC1 protein_coding protein_coding 2.372199 3.102755 2.466599 0.1860709 0.2208472 -0.3298318 0.4264455 0.52518269 0.5905793 0.05390271 0.07807758 0.1663232 0.17853750 0.17133333 0.2460333 0.0747000 0.512340759 0.007393045 FALSE TRUE
ENST00000584471 ENSG00000154217 HEK293_OSMI2_2hA HEK293_TMG_2hB PITPNC1 protein_coding protein_coding 2.372199 3.102755 2.466599 0.1860709 0.2208472 -0.3298318 0.1344481 0.08942942 0.3719911 0.08942942 0.21655802 1.9417942 0.05524583 0.02633333 0.1376000 0.1112667 0.545462388 0.007393045 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000154217 E001 8.9793630 0.0157048406 9.109062e-04 3.652242e-03 17 67377281 67377458 178 + 1.200 0.793 -1.511
ENSG00000154217 E002 87.5927448 0.0003900257 4.294577e-08 4.695532e-07 17 67377459 67378202 744 + 2.048 1.838 -0.706
ENSG00000154217 E003 0.5858876 0.0189396510 1.054910e-01 1.988493e-01 17 67378966 67379392 427 + 0.371 0.092 -2.519
ENSG00000154217 E004 0.0000000       17 67457538 67457823 286 +      
ENSG00000154217 E005 0.0000000       17 67459661 67459792 132 +      
ENSG00000154217 E006 51.0056571 0.0005254182 4.839510e-03 1.548387e-02 17 67532802 67532904 103 + 1.781 1.635 -0.494
ENSG00000154217 E007 55.5198222 0.0004569056 3.827545e-01 5.269170e-01 17 67532905 67532950 46 + 1.754 1.705 -0.167
ENSG00000154217 E008 69.8129317 0.0004774566 4.892298e-01 6.266879e-01 17 67552257 67552345 89 + 1.847 1.810 -0.124
ENSG00000154217 E009 8.3918458 0.0020753337 8.346683e-01 8.965093e-01 17 67553186 67553609 424 + 0.937 0.954 0.066
ENSG00000154217 E010 45.1790544 0.0005528452 9.429312e-01 9.682088e-01 17 67553610 67553617 8 + 1.636 1.632 -0.013
ENSG00000154217 E011 79.1472528 0.0003359011 2.332119e-01 3.662425e-01 17 67578186 67578257 72 + 1.844 1.885 0.140
ENSG00000154217 E012 96.5916633 0.0003428651 8.265112e-02 1.637011e-01 17 67632143 67632238 96 + 1.918 1.975 0.191
ENSG00000154217 E013 0.2214452 0.0421582613 8.636161e-01   17 67632239 67632421 183 + 0.001 0.092 7.215
ENSG00000154217 E014 131.7327609 0.0002537216 3.735548e-03 1.239860e-02 17 67669508 67669663 156 + 2.030 2.114 0.282
ENSG00000154217 E015 86.4851585 0.0003284727 1.796895e-04 8.766615e-04 17 67675479 67675542 64 + 1.803 1.947 0.487
ENSG00000154217 E016 79.9985023 0.0004684823 1.069942e-04 5.542721e-04 17 67687066 67687184 119 + 1.764 1.922 0.532
ENSG00000154217 E017 159.7684857 0.0090217774 1.903881e-01 3.142508e-01 17 67692572 67697256 4685 + 2.220 2.146 -0.247