ENSG00000154174

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000284320 ENSG00000154174 HEK293_OSMI2_2hA HEK293_TMG_2hB TOMM70 protein_coding protein_coding 41.50385 15.49236 66.86857 1.036821 1.781177 2.109056 34.526932 14.6359528 56.515860 0.85262907 1.472655 1.948409 0.8614125 0.94576667 0.8452667 -0.1005 6.315986e-07 1.282438e-16 FALSE TRUE
MSTRG.23353.3 ENSG00000154174 HEK293_OSMI2_2hA HEK293_TMG_2hB TOMM70 protein_coding   41.50385 15.49236 66.86857 1.036821 1.781177 2.109056 5.276422 0.3111605 7.823168 0.08003351 0.140302 4.608230 0.0969625 0.01983333 0.1170333 0.0972 1.282438e-16 1.282438e-16 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000154174 E001 2.475586 0.0060141676 3.574656e-01 5.018774e-01 3 100363215 100363430 216 - 0.515 0.363 -0.797
ENSG00000154174 E002 1958.404194 0.0065073611 9.878270e-05 5.161795e-04 3 100363431 100365717 2287 - 3.179 3.328 0.493
ENSG00000154174 E003 8.448767 0.0131886358 3.319756e-01 4.755989e-01 3 100367767 100368043 277 - 0.935 0.837 -0.378
ENSG00000154174 E004 363.284963 0.0005655097 1.003576e-01 1.910211e-01 3 100368044 100368166 123 - 2.468 2.548 0.269
ENSG00000154174 E005 318.072179 0.0001910806 6.235776e-01 7.401334e-01 3 100369038 100369135 98 - 2.418 2.471 0.179
ENSG00000154174 E006 2.822523 0.0079684306 9.406221e-01 9.666865e-01 3 100369136 100369178 43 - 0.515 0.558 0.203
ENSG00000154174 E007 5.491456 0.0065521495 2.533819e-01 3.898990e-01 3 100372006 100372605 600 - 0.780 0.630 -0.623
ENSG00000154174 E008 334.886928 0.0001981822 7.274894e-01 8.201752e-01 3 100372606 100372722 117 - 2.440 2.489 0.166
ENSG00000154174 E009 339.142740 0.0001455746 1.158467e-01 2.140159e-01 3 100373538 100373645 108 - 2.457 2.463 0.020
ENSG00000154174 E010 1.065312 0.0120998414 6.376917e-01 7.515321e-01 3 100373646 100373725 80 - 0.295 0.218 -0.574
ENSG00000154174 E011 348.964885 0.0003460724 2.794459e-01 4.192020e-01 3 100375018 100375152 135 - 2.466 2.483 0.054
ENSG00000154174 E012 7.330513 0.0026487340 5.705905e-01 6.970336e-01 3 100377416 100377704 289 - 0.835 0.946 0.424
ENSG00000154174 E013 466.271122 0.0014638877 2.079779e-01 3.360638e-01 3 100377705 100377912 208 - 2.594 2.601 0.022
ENSG00000154174 E014 438.440289 0.0023511488 6.186201e-02 1.297532e-01 3 100381615 100381763 149 - 2.573 2.555 -0.060
ENSG00000154174 E015 1.364190 0.0106537992 4.234873e-01 5.665342e-01 3 100381764 100381772 9 - 0.361 0.218 -0.990
ENSG00000154174 E016 343.148547 0.0001584485 2.985446e-07 2.764339e-06 3 100384479 100384588 110 - 2.480 2.401 -0.265
ENSG00000154174 E017 342.811353 0.0039091647 1.669893e-04 8.221535e-04 3 100386218 100386344 127 - 2.485 2.379 -0.351
ENSG00000154174 E018 308.894039 0.0020334687 1.455415e-08 1.740902e-07 3 100386805 100386978 174 - 2.448 2.297 -0.501
ENSG00000154174 E019 0.000000       3 100389957 100390011 55 -      
ENSG00000154174 E020 332.648125 0.0064681792 2.356342e-05 1.437410e-04 3 100400626 100401089 464 - 2.480 2.324 -0.522