ENSG00000154122

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000284268 ENSG00000154122 HEK293_OSMI2_2hA HEK293_TMG_2hB ANKH protein_coding protein_coding 24.28547 33.53245 20.62377 1.120588 0.8262097 -0.7009808 3.054879 3.148526 3.227086 0.1049233 0.09504171 0.03544443 0.1326500 0.0941000 0.1572333 0.06313333 0.002084955 0.001065376 FALSE TRUE
ENST00000502585 ENSG00000154122 HEK293_OSMI2_2hA HEK293_TMG_2hB ANKH protein_coding retained_intron 24.28547 33.53245 20.62377 1.120588 0.8262097 -0.7009808 6.068823 10.007934 4.010887 0.2100490 0.29591176 -1.31699921 0.2390167 0.2994000 0.1941667 -0.10523333 0.001065376 0.001065376 FALSE TRUE
ENST00000503939 ENSG00000154122 HEK293_OSMI2_2hA HEK293_TMG_2hB ANKH protein_coding processed_transcript 24.28547 33.53245 20.62377 1.120588 0.8262097 -0.7009808 11.123761 15.772800 8.851033 0.6835421 0.36201651 -0.83280637 0.4571000 0.4700333 0.4292667 -0.04076667 0.293713205 0.001065376 FALSE TRUE
MSTRG.25957.2 ENSG00000154122 HEK293_OSMI2_2hA HEK293_TMG_2hB ANKH protein_coding   24.28547 33.53245 20.62377 1.120588 0.8262097 -0.7009808 2.410030 2.919837 2.368120 0.2814687 0.11391449 -0.30099887 0.1013125 0.0867000 0.1150333 0.02833333 0.210795211 0.001065376 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000154122 E001 3.5071941 0.1155119327 2.788365e-01 4.185033e-01 5 14704800 14705071 272 - 0.762 0.582 -0.763
ENSG00000154122 E002 33.4611021 0.0052671123 6.315296e-13 1.601196e-11 5 14705072 14707342 2271 - 1.772 1.284 -1.674
ENSG00000154122 E003 300.0313240 0.0009792939 9.032040e-07 7.570872e-06 5 14707343 14710547 3205 - 2.530 2.417 -0.375
ENSG00000154122 E004 165.3404568 0.0002165509 4.380813e-10 6.932308e-09 5 14710548 14711001 454 - 2.302 2.135 -0.558
ENSG00000154122 E005 78.4057909 0.0003892200 1.682129e-03 6.225688e-03 5 14711002 14711087 86 - 1.958 1.835 -0.412
ENSG00000154122 E006 190.7773269 0.0011607490 1.303275e-01 2.346140e-01 5 14711088 14711310 223 - 2.234 2.283 0.164
ENSG00000154122 E007 149.5238957 0.0012161319 4.007180e-05 2.309126e-04 5 14712874 14712973 100 - 2.061 2.204 0.478
ENSG00000154122 E008 1.1351534 0.4117913863 9.803454e-01 9.917207e-01 5 14712974 14713543 570 - 0.341 0.313 -0.180
ENSG00000154122 E009 172.1066786 0.0037544235 1.394393e-02 3.809500e-02 5 14713544 14713667 124 - 2.152 2.253 0.340
ENSG00000154122 E010 1.2660437 0.1468364791 6.103822e-01 7.297062e-01 5 14713668 14716525 2858 - 0.415 0.311 -0.608
ENSG00000154122 E011 179.4373974 0.0026679087 3.547486e-02 8.256779e-02 5 14716706 14716835 130 - 2.183 2.263 0.267
ENSG00000154122 E012 0.0000000       5 14716836 14717088 253 -      
ENSG00000154122 E013 3.5866993 0.0058845362 7.496988e-01 8.365747e-01 5 14741292 14741378 87 - 0.622 0.673 0.219
ENSG00000154122 E014 1.2220365 0.0120774485 5.623530e-01 6.899827e-01 5 14741457 14741826 370 - 0.414 0.314 -0.590
ENSG00000154122 E015 185.2740827 0.0020986915 3.031478e-02 7.258550e-02 5 14741827 14741922 96 - 2.200 2.277 0.258
ENSG00000154122 E016 158.4724596 0.0002426719 9.125935e-04 3.658311e-03 5 14745870 14745962 93 - 2.116 2.216 0.336
ENSG00000154122 E017 179.8035924 0.0027543893 1.110347e-02 3.141502e-02 5 14749172 14749306 135 - 2.173 2.268 0.318
ENSG00000154122 E018 212.7236958 0.0014486705 1.239933e-02 3.449564e-02 5 14751069 14751239 171 - 2.256 2.334 0.261
ENSG00000154122 E019 154.2143723 0.0064749168 4.272037e-01 5.700271e-01 5 14755861 14755944 84 - 2.143 2.184 0.135
ENSG00000154122 E020 149.6680852 0.0020699011 3.903753e-01 5.343487e-01 5 14758480 14758598 119 - 2.134 2.168 0.114
ENSG00000154122 E021 3.3214359 0.0045786970 3.641246e-01 5.085166e-01 5 14768162 14768541 380 - 0.698 0.563 -0.586
ENSG00000154122 E022 5.1951225 0.0031569941 5.520814e-01 6.814478e-01 5 14768616 14768974 359 - 0.818 0.742 -0.306
ENSG00000154122 E023 74.1438931 0.0097077665 8.820763e-01 9.284687e-01 5 14768975 14769006 32 - 1.859 1.852 -0.024
ENSG00000154122 E024 155.0603251 0.0066669667 2.477149e-01 3.832522e-01 5 14769007 14769191 185 - 2.203 2.154 -0.164
ENSG00000154122 E025 0.0000000       5 14788116 14788217 102 -      
ENSG00000154122 E026 0.1482932 0.0413927269 7.029856e-01   5 14868415 14868708 294 - 0.000 0.102 9.780
ENSG00000154122 E027 0.1482932 0.0413927269 7.029856e-01   5 14868709 14869030 322 - 0.000 0.102 9.780
ENSG00000154122 E028 0.0000000       5 14869031 14870182 1152 -      
ENSG00000154122 E029 0.4741261 0.0208118479 3.550664e-01 4.994118e-01 5 14870183 14871351 1169 - 0.255 0.102 -1.586
ENSG00000154122 E030 128.5943324 0.0024524160 3.542777e-05 2.068443e-04 5 14871352 14871778 427 - 2.185 2.030 -0.519