ENSG00000153914

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000334121 ENSG00000153914 HEK293_OSMI2_2hA HEK293_TMG_2hB SREK1 protein_coding protein_coding 18.89538 3.906663 30.1212 0.3046972 1.467217 2.943561 8.71857540 2.5789539 11.063453 0.17232438 0.23309358 2.096664 0.51778333 0.66143333 0.3685333 -0.29290000 8.525709e-11 4.482252e-14 FALSE TRUE
ENST00000520058 ENSG00000153914 HEK293_OSMI2_2hA HEK293_TMG_2hB SREK1 protein_coding retained_intron 18.89538 3.906663 30.1212 0.3046972 1.467217 2.943561 2.21080746 0.1610080 4.551561 0.05308348 0.77043161 4.737391 0.08396667 0.04103333 0.1494000 0.10836667 1.854716e-03 4.482252e-14 FALSE TRUE
ENST00000522912 ENSG00000153914 HEK293_OSMI2_2hA HEK293_TMG_2hB SREK1 protein_coding nonsense_mediated_decay 18.89538 3.906663 30.1212 0.3046972 1.467217 2.943561 0.92208104 0.3623345 1.408800 0.20498220 0.18344456 1.930000 0.05462917 0.08620000 0.0464000 -0.03980000 8.963371e-01 4.482252e-14 FALSE TRUE
ENST00000523655 ENSG00000153914 HEK293_OSMI2_2hA HEK293_TMG_2hB SREK1 protein_coding nonsense_mediated_decay 18.89538 3.906663 30.1212 0.3046972 1.467217 2.943561 2.30899489 0.1524862 4.656511 0.08269938 0.73139316 4.843955 0.10590417 0.03626667 0.1540333 0.11776667 2.296564e-02 4.482252e-14   FALSE
ENST00000524111 ENSG00000153914 HEK293_OSMI2_2hA HEK293_TMG_2hB SREK1 protein_coding retained_intron 18.89538 3.906663 30.1212 0.3046972 1.467217 2.943561 1.86521753 0.0000000 4.071297 0.00000000 0.06352687 8.672884 0.06938750 0.00000000 0.1358000 0.13580000 4.482252e-14 4.482252e-14 FALSE FALSE
MSTRG.26289.10 ENSG00000153914 HEK293_OSMI2_2hA HEK293_TMG_2hB SREK1 protein_coding   18.89538 3.906663 30.1212 0.3046972 1.467217 2.943561 0.07846898 0.2018501 0.000000 0.07685152 0.00000000 -4.404972 0.01910417 0.05346667 0.0000000 -0.05346667 1.005703e-05 4.482252e-14 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000153914 E001 0.0000000       5 66139971 66140011 41 +      
ENSG00000153914 E002 3.4195840 0.0051356775 2.398995e-01 3.740258e-01 5 66144218 66144263 46 + 0.491 0.766 1.204
ENSG00000153914 E003 5.5316729 0.0030132085 4.366280e-01 5.786692e-01 5 66144264 66144299 36 + 0.670 0.872 0.812
ENSG00000153914 E004 6.4279662 0.0026056915 7.459439e-01 8.338408e-01 5 66144300 66144306 7 + 0.738 0.872 0.531
ENSG00000153914 E005 6.5794817 0.0024276053 7.999765e-01 8.724655e-01 5 66144307 66144311 5 + 0.748 0.872 0.490
ENSG00000153914 E006 9.1958499 0.0019628571 2.218724e-01 3.526323e-01 5 66144312 66144314 3 + 0.908 0.823 -0.329
ENSG00000153914 E007 14.0102834 0.0012802066 8.625863e-02 1.694133e-01 5 66144315 66144335 21 + 1.075 0.957 -0.434
ENSG00000153914 E008 44.0553100 0.0004642321 2.545583e-02 6.281627e-02 5 66144336 66144442 107 + 1.538 1.478 -0.205
ENSG00000153914 E009 56.5036251 0.0004006955 1.072669e-02 3.051398e-02 5 66144443 66144537 95 + 1.643 1.581 -0.211
ENSG00000153914 E010 0.1515154 0.0434527026 1.000000e+00   5 66144835 66145183 349 + 0.054 0.000 -14.115
ENSG00000153914 E011 38.4819297 0.0005238016 5.077700e-02 1.105568e-01 5 66153463 66153490 28 + 1.477 1.428 -0.169
ENSG00000153914 E012 56.2287977 0.0011906540 7.272536e-03 2.193257e-02 5 66153491 66153567 77 + 1.640 1.562 -0.265
ENSG00000153914 E013 44.9215764 0.0006635265 1.307431e-02 3.608257e-02 5 66153568 66153596 29 + 1.546 1.466 -0.274
ENSG00000153914 E014 14.8468442 0.0166103864 2.824947e-04 1.308439e-03 5 66153597 66153790 194 + 1.130 0.627 -1.949
ENSG00000153914 E015 106.8384356 0.0361955919 2.937315e-05 1.751654e-04 5 66156065 66158126 2062 + 1.945 1.589 -1.207
ENSG00000153914 E016 9.8374984 0.0022673591 3.134482e-01 4.560395e-01 5 66158127 66158230 104 + 0.922 0.873 -0.188
ENSG00000153914 E017 34.5479448 0.0006588271 2.236224e-07 2.123645e-06 5 66158231 66158699 469 + 1.468 1.060 -1.435
ENSG00000153914 E018 18.2274368 0.0050462311 1.890053e-02 4.911659e-02 5 66158700 66158808 109 + 1.181 0.995 -0.673
ENSG00000153914 E019 25.7278590 0.0013410404 3.419799e-01 4.859626e-01 5 66158809 66158932 124 + 1.296 1.309 0.045
ENSG00000153914 E020 6.8837340 0.0024659527 2.625886e-01 4.003719e-01 5 66158933 66159105 173 + 0.796 0.702 -0.381
ENSG00000153914 E021 5.4777289 0.0029279407 5.365184e-03 1.692281e-02 5 66159106 66159218 113 + 0.758 0.257 -2.551
ENSG00000153914 E022 82.6758054 0.0003259609 1.169889e-01 2.156625e-01 5 66159219 66159334 116 + 1.789 1.811 0.074
ENSG00000153914 E023 104.6769338 0.0002705974 1.651852e-02 4.388183e-02 5 66162109 66162273 165 + 1.898 1.890 -0.026
ENSG00000153914 E024 124.7095180 0.0005764501 1.823639e-02 4.764623e-02 5 66162414 66162592 179 + 1.970 1.972 0.005
ENSG00000153914 E025 12.0798884 0.0014098084 1.087976e-02 3.088078e-02 5 66162593 66163582 990 + 1.035 0.766 -1.025
ENSG00000153914 E026 2.2133430 0.0065389015 3.479482e-02 8.125253e-02 5 66163583 66163791 209 + 0.473 0.000 -18.021
ENSG00000153914 E027 116.6008138 0.0004319159 1.448183e-02 3.931924e-02 5 66163792 66163922 131 + 1.942 1.937 -0.017
ENSG00000153914 E028 12.0556028 0.0180386801 8.724577e-03 2.559733e-02 5 66163923 66164607 685 + 1.036 0.702 -1.288
ENSG00000153914 E029 12.0131783 0.0014293931 3.222126e-02 7.627964e-02 5 66164608 66164782 175 + 1.025 0.823 -0.764
ENSG00000153914 E030 126.4388053 0.0014052800 9.890020e-03 2.849244e-02 5 66164783 66164897 115 + 1.979 1.965 -0.048
ENSG00000153914 E031 48.2046330 0.0005614005 2.096558e-07 2.002251e-06 5 66164898 66170050 5153 + 1.602 1.291 -1.071
ENSG00000153914 E032 142.7047190 0.0002238155 4.737738e-02 1.045012e-01 5 66170051 66170170 120 + 2.024 2.050 0.087
ENSG00000153914 E033 236.0075285 0.0021110750 4.642679e-03 1.493888e-02 5 66170585 66170947 363 + 2.246 2.244 -0.007
ENSG00000153914 E034 1.9626498 0.0083623652 8.661235e-01 9.177959e-01 5 66174624 66174945 322 + 0.388 0.417 0.159
ENSG00000153914 E035 119.6351218 0.0005778338 1.671634e-01 2.845084e-01 5 66174946 66175041 96 + 1.944 1.987 0.144
ENSG00000153914 E036 0.0000000       5 66177327 66177513 187 +      
ENSG00000153914 E037 67.4308814 0.0026978387 5.985605e-01 7.201225e-01 5 66177514 66177516 3 + 1.692 1.761 0.234
ENSG00000153914 E038 167.7612256 0.0056038116 7.852977e-01 8.623730e-01 5 66177517 66177658 142 + 2.076 2.191 0.385
ENSG00000153914 E039 895.5438902 0.0102745058 4.319891e-10 6.844446e-09 5 66178719 66183615 4897 + 2.752 3.070 1.058