ENSG00000153896

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000587354 ENSG00000153896 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF599 protein_coding protein_coding 7.679446 12.33497 4.660057 0.1604953 0.5279728 -1.402411 0.7141696 0.5143555 0.7222198 0.06098652 0.04364814 0.4817314 0.11836667 0.041733333 0.1568000 0.11506667 1.858395e-06 9.091812e-10   FALSE
ENST00000588760 ENSG00000153896 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF599 protein_coding protein_coding 7.679446 12.33497 4.660057 0.1604953 0.5279728 -1.402411 5.9826852 10.9156613 2.2980617 0.39369185 0.39540168 -2.2429669 0.73170417 0.884400000 0.5231667 -0.36123333 1.857941e-02 9.091812e-10   FALSE
ENST00000673678 ENSG00000153896 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF599 protein_coding nonsense_mediated_decay 7.679446 12.33497 4.660057 0.1604953 0.5279728 -1.402411 0.2546383 0.1188595 0.4679357 0.01752880 0.04679867 1.8910176 0.04634167 0.009633333 0.1008667 0.09123333 9.091812e-10 9.091812e-10 FALSE TRUE
MSTRG.16957.5 ENSG00000153896 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF599 protein_coding   7.679446 12.33497 4.660057 0.1604953 0.5279728 -1.402411 0.2403054 0.0000000 0.9820802 0.00000000 0.74806571 6.6323848 0.04092500 0.000000000 0.1816333 0.18163333 1.143292e-01 9.091812e-10   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000153896 E001 0.8385790 0.1656428430 6.713687e-01 7.776087e-01 19 34757447 34757563 117 - 0.171 0.274 0.868
ENSG00000153896 E002 0.0000000       19 34758073 34758078 6 -      
ENSG00000153896 E003 16.0249097 0.0012640731 6.463772e-22 5.100134e-20 19 34758079 34760559 2481 - 1.586 0.827 -2.715
ENSG00000153896 E004 12.4072455 0.0015074651 1.043857e-02 2.983549e-02 19 34762795 34763505 711 - 1.227 1.019 -0.748
ENSG00000153896 E005 2.8141166 0.0087911803 3.565803e-05 2.080267e-04 19 34763506 34763555 50 - 0.889 0.274 -2.937
ENSG00000153896 E006 29.8878284 0.0006982682 4.436648e-19 2.549316e-17 19 34763556 34765043 1488 - 1.743 1.243 -1.721
ENSG00000153896 E007 11.9118151 0.0020456486 6.445253e-03 1.978024e-02 19 34765044 34765641 598 - 1.227 1.000 -0.818
ENSG00000153896 E008 10.7137688 0.0508147777 6.313582e-01 7.463662e-01 19 34766968 34767022 55 - 0.940 1.057 0.433
ENSG00000153896 E009 73.7635467 0.0185431596 1.366001e-06 1.101733e-05 19 34767023 34767315 293 - 1.496 1.906 1.390
ENSG00000153896 E010 53.2181735 0.0005155104 8.838430e-05 4.674262e-04 19 34767316 34767411 96 - 1.496 1.745 0.847
ENSG00000153896 E011 66.8013028 0.0042058933 2.609328e-03 9.092785e-03 19 34769429 34769555 127 - 1.627 1.824 0.666
ENSG00000153896 E012 10.0634802 0.0024289236 3.943589e-02 8.998724e-02 19 34772336 34772473 138 - 1.115 0.929 -0.684
ENSG00000153896 E013 0.9201516 0.0141734774 3.002900e-01 4.419562e-01 19 34772474 34772823 350 - 0.388 0.220 -1.131
ENSG00000153896 E014 100.5219820 0.0057881168 7.695739e-02 1.546224e-01 19 34772824 34773229 406 - 1.870 1.983 0.381